KEGG   ENZYME: 3.1.1.14Help
Entry
EC 3.1.1.14                 Enzyme                                 

Name
chlorophyllase;
CLH;
Chlase
Class
Hydrolases;
Acting on ester bonds;
Carboxylic-ester hydrolases
BRITE hierarchy
Sysname
chlorophyll chlorophyllidohydrolase
Reaction(IUBMB)
chlorophyll + H2O = phytol + chlorophyllide
Reaction(KEGG)
(other) R05618 R09068
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Substrate
chlorophyll [CPD:C01793];
H2O [CPD:C00001]
Product
phytol [CPD:C01389];
chlorophyllide [CPD:C02139]
Comment
Chlorophyllase has been found in higher plants, diatoms, and in the green algae Chlorella [3]. This enzyme forms part of the chlorophyll degradation pathway and is thought to take part in de-greening processes such as fruit ripening, leaf senescence and flowering, as well as in the turnover and homeostasis of chlorophyll [4]. This enzyme acts preferentially on chlorophyll a but will also accept chlorophyll b and pheophytins as substrates [5]. Ethylene and methyl jasmonate, which are known to accelerate senescence in many species, can enhance the activity of the hormone-inducible form of this enzyme [5].
History
EC 3.1.1.14 created 1961, modified 2007
Pathway
ec00860  Porphyrin and chlorophyll metabolism
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K08099  chlorophyllase
Genes
BFO: BRAFLDRAFT_94943
LGI: LOTGIDRAFT_163441
MYI: 110462918
ATH: AT1G19670(CLH1) AT5G43860(CLH2)
ALY: ARALYDRAFT_472193(ATCLH1) ARALYDRAFT_494619(ATCLH2)
CRB: 17877061 17898197
CSAT: 104725070 104725071 104740665 104756312 104760568 104760570 104771535 104772775 104776081
EUS: EUTSA_v10003248mg EUTSA_v10008201mg
BRP: 103839225 103848843 103872768(Chlase)
BNA: 106346945 106366642 106407289 106416159 106451729
THJ: 104801988
CPAP: 110815852
TCC: 18603049
CAM: 101490133
LJA: Lj0g3v0271829.1(Lj0g3v0271829.1) Lj2g3v0704890.1(Lj2g3v0704890.1)
CMAX: 111499382
CMOS: 111459924
CPEP: 111807242
RCU: 8280860
OSA: 4348648
DOSA: Os02t0286700-00(Os02g0286700) Os10t0419600-00(Os10g0419600)
OBR: 102713942
ATS: 109772274(LOC109772274) 109772321(LOC109772321) 109776391(LOC109776391) 109783103(LOC109783103)
MUS: 103978829
PEQ: 110032436
PPP: 112273217
 » show all
Taxonomy
Reference
1  [PMID:13715233]
  Authors
HOLDEN M.
  Title
The breakdown of chlorophyll by chlorophyllase.
  Journal
Biochem J 78:359-64 (1961)
Reference
2  [PMID:13756631]
  Authors
KLEIN AO, VISHNIAC W.
  Title
Activity and partial purification of chlorophyllase in aqueous systems.
  Journal
J Biol Chem 236:2544-7 (1961)
Reference
3  [PMID:10611389]
  Authors
Tsuchiya T, Ohta H, Okawa K, Iwamatsu A, Shimada H, Masuda T, Takamiya K.
  Title
Cloning of chlorophyllase, the key enzyme in chlorophyll degradation: finding of a lipase motif and the induction by methyl jasmonate.
  Journal
Proc Natl Acad Sci U S A 96:15362-7 (1999)
DOI:10.1073/pnas.96.26.15362
  Sequence
Reference
4  [PMID:16610227]
  Authors
Okazawa A, Tango L, Itoh Y, Fukusaki E, Kobayashi A.
  Title
Characterization and subcellular localization of chlorophyllase from Ginkgo biloba.
  Journal
Z Naturforsch [C] 61:111-7 (2006)
Reference
5  [PMID:16669755]
  Authors
Hortensteiner S.
  Title
Chlorophyll degradation during senescence.
  Journal
Annu Rev Plant Biol 57:55-77 (2006)
DOI:10.1146/annurev.arplant.57.032905.105212
Other DBs
ExplorEnz - The Enzyme Database: 3.1.1.14
IUBMB Enzyme Nomenclature: 3.1.1.14
ExPASy - ENZYME nomenclature database: 3.1.1.14
BRENDA, the Enzyme Database: 3.1.1.14
CAS: 9025-96-1

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