KEGG   ENZYME: 3.1.1.64Help
Entry
EC 3.1.1.64                 Enzyme                                 

Name
retinoid isomerohydrolase;
all-trans-retinyl-palmitate hydrolase (ambiguous);
retinol isomerase (ambiguous);
all-trans-retinol isomerase:hydrolase (ambiguous);
all-trans-retinylester 11-cis isomerohydrolase;
RPE65 (gene name)
Class
Hydrolases;
Acting on ester bonds;
Carboxylic-ester hydrolases
BRITE hierarchy
Sysname
all-trans-retinyl ester acylhydrolase, 11-cis retinol forming
Reaction(IUBMB)
an all-trans-retinyl ester + H2O = 11-cis-retinol + a fatty acid [RN:R08388]
Reaction(KEGG)
Substrate
all-trans-retinyl ester [CPD:C02075];
H2O [CPD:C00001]
Product
11-cis-retinol [CPD:C00899];
fatty acid [CPD:C00162]
Comment
This enzyme, which operates in the retinal pigment epithelium (RPE), catalyses the cleavage and isomerization of all-trans-retinyl fatty acid esters to 11-cis-retinol, a key step in the regeneration of the visual chromophore in the vertebrate visual cycle [4]. Interaction of the enzyme with the membrane is critical for its enzymic activity [6].
History
EC 3.1.1.64 created 1989 (EC 5.2.1.7 created 1989, incorporated 2011), modified 2011
Pathway
ec00830  Retinol metabolism
Orthology
K11158  retinoid isomerohydrolase / lutein isomerase
Genes
HSA: 6121(RPE65)
PTR: 744567(RPE65)
PPS: 100994777(RPE65)
GGO: 101152566(RPE65)
PON: 100453879(RPE65)
NLE: 100588685(RPE65)
MCC: 701764(RPE65)
MCF: 102122358(RPE65)
CSAB: 103224643(RPE65)
RRO: 104675297(RPE65)
RBB: 108532954(RPE65)
CJC: 100397525(RPE65)
SBQ: 101044000(RPE65)
MMU: 19892(Rpe65)
RNO: 89826(Rpe65)
CGE: 100767488(Rpe65)
NGI: 103746374(Rpe65)
HGL: 101716560(Rpe65)
CCAN: 109698727(Rpe65)
OCU: 100352270(RPE65)
TUP: 102499334(RPE65)
CFA: 403803(RPE65)
AML: 100469948(RPE65)
UMR: 103663024(RPE65)
ORO: 101373037(RPE65)
FCA: 101090034(RPE65)
PTG: 102952948(RPE65)
AJU: 106982702(RPE65)
BTA: 282043(RPE65)
BOM: 102280727(RPE65)
BIU: 109557070(RPE65)
PHD: 102327439(RPE65)
CHX: 102174353(RPE65)
OAS: 101116520(RPE65)
SSC: 100516743(RPE65)
CFR: 102504541(RPE65)
CDK: 105096629(RPE65)
BACU: 103007082(RPE65)
LVE: 103082229(RPE65)
OOR: 101280600(RPE65)
ECB: 100068802(RPE65)
EPZ: 103556149(RPE65)
EAI: 106847823(RPE65)
MYB: 102264093(RPE65)
MYD: 102757349(RPE65)
HAI: 109384924(RPE65)
RSS: 109447724(RPE65)
PALE: 102887612(RPE65)
LAV: 100664258(RPE65)
TMU: 101352162
MDO: 100030329(RPE65)
SHR: 100934519(RPE65)
OAA: 100081441(RPE65)
GGA: 395700(RPE65)
MGP: 100539517(RPE65)
CJO: 107317795(RPE65)
APLA: 101801077(RPE65) 106014305
ACYG: 106043625(RPE65)
TGU: 100219959(RPE65)
GFR: 102043093(RPE65)
FAB: 101808678(RPE65)
PHI: 102103362(RPE65)
PMAJ: 107208456(RPE65)
CCAE: 111932946(RPE65)
CCW: 104695122(RPE65)
FPG: 101918066(RPE65)
FCH: 102049984(RPE65)
CLV: 102085010(RPE65)
EGZ: 104123866(RPE65)
AAM: 106486524(RPE65)
ASN: 102369965(RPE65)
AMJ: 102560040(RPE65)
PSS: 102456788(RPE65)
CMY: 102935763(RPE65)
CPIC: 101936194(RPE65)
ACS: 100556137(rpe65)
PVT: 110079873(RPE65)
PBI: 103048404(RPE65)
GJA: 107114347(RPE65)
XLA: 379961(rpe65.S) 447613(rpe65.L)
XTR: 100145692(rpe65)
NPR: 108795952(RPE65)
DRE: 100004076(rpe65c) 393724(rpe65a)
IPU: 108267184(rpe65)
AMEX: 103023405(rpe65)
TRU: 101062285(rpe65) 101065379
LCO: 104935905(rpe65) 104936272
OLA: 101163729 101172024(rpe65)
XMA: 102217285(rpe65) 102231785
NFU: 107383888(rpe65) 107393007
LCF: 108879593 108883387(rpe65)
SDU: 111223483 111231109(rpe65)
HCQ: 109521243(rpe65) 109525455
ELS: 105011682(rpe65) 105019406
SFM: 108919070(rpe65) 108935006
LCM: 102364001(RPE65)
CMK: 103189136(rpe65)
ADF: 107351382
 » show all
Taxonomy
Reference
1  [PMID:3793734]
  Authors
Blaner WS, Das SR, Gouras P, Flood MT.
  Title
Hydrolysis of 11-cis- and all-trans-retinyl palmitate by homogenates of human retinal epithelial cells.
  Journal
J Biol Chem 262:53-8 (1987)
Reference
2  [PMID:3494246]
  Authors
Bernstein PS, Law WC, Rando RR.
  Title
Isomerization of all-trans-retinoids to 11-cis-retinoids in vitro.
  Journal
Proc Natl Acad Sci U S A 84:1849-53 (1987)
DOI:10.1073/pnas.84.7.1849
Reference
3  [PMID:3603006]
  Authors
Bridges CD, Alvarez RA.
  Title
The visual cycle operates via an isomerase acting on all-trans retinol in the pigment epithelium.
  Journal
Science 236:1678-80 (1987)
DOI:10.1126/science.3603006
  Sequence
[rno:89826]
Reference
4  [PMID:16116091]
  Authors
Moiseyev G, Chen Y, Takahashi Y, Wu BX, Ma JX.
  Title
RPE65 is the isomerohydrolase in the retinoid visual cycle.
  Journal
Proc Natl Acad Sci U S A 102:12413-8 (2005)
DOI:10.1073/pnas.0503460102
  Sequence
[hsa:6121]
Reference
5  [PMID:19490105]
  Authors
Nikolaeva O, Takahashi Y, Moiseyev G, Ma JX
  Title
Purified RPE65 shows isomerohydrolase activity after reassociation with a phospholipid membrane.
  Journal
FEBS J 276:3020-30 (2009)
DOI:10.1111/j.1742-4658.2009.07021.x
  Sequence
[gga:395700]
Reference
6  [PMID:20100834]
  Authors
Golczak M, Kiser PD, Lodowski DT, Maeda A, Palczewski K
  Title
Importance of membrane structural integrity for RPE65 retinoid isomerization activity.
  Journal
J Biol Chem 285:9667-82 (2010)
DOI:10.1074/jbc.M109.063941
  Sequence
[bta:282043]
Other DBs
ExplorEnz - The Enzyme Database: 3.1.1.64
IUBMB Enzyme Nomenclature: 3.1.1.64
ExPASy - ENZYME nomenclature database: 3.1.1.64
BRENDA, the Enzyme Database: 3.1.1.64
CAS: 106389-24-6

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