KEGG   ENZYME: 3.1.1.79
Entry
EC 3.1.1.79                 Enzyme                                 

Name
hormone-sensitive lipase;
HSL
Class
Hydrolases;
Acting on ester bonds;
Carboxylic-ester hydrolases
Sysname
diacylglycerol acylhydrolase
Reaction(IUBMB)
(1) diacylglycerol + H2O = monoacylglycerol + a carboxylate [RN:R05209];
(2) triacylglycerol + H2O = diacylglycerol + a carboxylate [RN:R01369];
(3) monoacylglycerol + H2O = glycerol + a carboxylate [RN:R07293]
Reaction(KEGG)
Substrate
diacylglycerol [CPD:C00165];
H2O [CPD:C00001];
triacylglycerol [CPD:C00422];
monoacylglycerol [CPD:C01885]
Product
monoacylglycerol [CPD:C01885];
carboxylate [CPD:C00060];
diacylglycerol [CPD:C00165];
glycerol [CPD:C00116]
Comment
This enzyme is a serine hydrolase. Compared with other lipases, hormone-sensitive lipase has a uniquely broad substrate specificity. It hydrolyses all acylglycerols (triacylglycerol, diacylglycerol and monoacylglycerol) [2,3,4] as well as cholesteryl esters [2,4], steroid fatty acid esters [5], retinyl esters [6] and p-nitrophenyl esters [4,7]. It exhibits a preference for the 1- or 3-ester bond of its acylglycerol substrate compared with the 2-ester bond [8]. The enzyme shows little preference for the fatty acids in the triacylglycerol, although there is some increase in activity with decreasing chain length. The enzyme activity is increased in response to hormones that elevate intracellular levels of cAMP.
History
EC 3.1.1.79 created 2004
Orthology
K07188  hormone-sensitive lipase
Genes
HSA: 3991(LIPE)
PTR: 456076(LIPE)
PPS: 100975071(LIPE)
GGO: 101145804(LIPE)
PON: 100445800(LIPE)
NLE: 100603864(LIPE)
MCC: 707997(LIPE)
MCF: 102143368(LIPE)
CSAB: 103234777(LIPE)
CATY: 105598066(LIPE)
PANU: 101023266(LIPE)
RRO: 104658678(LIPE)
RBB: 108513895(LIPE)
TFN: 117075239(LIPE)
PTEH: 111530380
CJC: 100387953(LIPE)
SBQ: 101052652(LIPE)
MMUR: 105868011(LIPE)
MMU: 16890(Lipe)
MCAL: 110298158(Lipe)
MPAH: 110336545(Lipe)
RNO: 25330(Lipe)
MCOC: 116101848(Lipe)
MUN: 110563910(Lipe)
CGE: 100755300(Lipe)
PLEU: 114685349(Lipe)
NGI: 103744591(Lipe)
HGL: 101709284(Lipe)
CCAN: 109691865(Lipe)
OCU: 100339941(LIPE)
OPI: 101528437(LIPE)
TUP: 102477502(LIPE)
CFA: 403607(LIPE)
VVP: 112931218(LIPE)
VLG: 121484344(LIPE)
AML: 100479605(LIPE)
UMR: 103680155(LIPE)
UAH: 113243521(LIPE)
ORO: 101384261(LIPE)
ELK: 111161566
MPUF: 101683381(LIPE)
EJU: 114197074(LIPE)
MLX: 117998053(LIPE)
FCA: 751818(LIPE)
PYU: 121018485(LIPE)
PBG: 122493988(LIPE)
PTG: 102952452(LIPE)
PPAD: 109257948(LIPE)
AJU: 106987707(LIPE)
HHV: 120243020(LIPE)
BTA: 286879(LIPE)
BOM: 102266815(LIPE)
BIU: 109572731(LIPE)
BBUB: 102389675(LIPE)
CHX: 100860801(LIPE)
OAS: 100169699(LIPE)
ODA: 120872376(LIPE)
CCAD: 122420167(LIPE)
SSC: 397583(LIPE)
CFR: 102520149
CBAI: 105062447(LIPE)
CDK: 105090211(LIPE)
BACU: 103006716(LIPE)
LVE: 103074607(LIPE)
OOR: 101277193(LIPE)
DLE: 111180817(LIPE)
PCAD: 102989235(LIPE)
ECB: 100069598(LIPE)
EPZ: 103558823(LIPE) 103563985
EAI: 106847941(LIPE)
MYB: 102247234(LIPE)
MYD: 102764534(LIPE)
MMYO: 118674384(LIPE)
MNA: 107534157(LIPE)
PKL: 118702302(LIPE)
HAI: 109374451(LIPE)
DRO: 112320229(LIPE)
SHON: 119000496(LIPE)
AJM: 119060132(LIPE)
MMF: 118639408(LIPE)
PALE: 102889858(LIPE)
RAY: 107502572(LIPE)
MJV: 108393430(LIPE)
TOD: 119249387(LIPE)
LAV: 100670874(LIPE)
TMU: 101361564
MDO: 103104890(LIPE)
SHR: 100926393(LIPE)
PCW: 110219706(LIPE)
OAA: 103167455(LIPE)
AAM: 106487870(LIPE)
AROW: 112971701(LIPE)
ASN: 102386893(LIPE)
AMJ: 102571969(LIPE)
CPIC: 101953217(LIPE)
TST: 117888400(LIPE)
CABI: 116818657(LIPE)
PVT: 110086763(LIPE)
PBI: 103054092(LIPE)
PMUR: 107289716(LIPE)
PGUT: 117677674(LIPE)
PMUA: 114603349(LIPE)
ZVI: 118087523(LIPE)
GJA: 107106853(LIPE) 107117837
XLA: 100174796(lipe.L) 108697769(lipe.S)
XTR: 733899(lipe)
NPR: 108799329(LIPE)
DRE: 557893(lipeb) 568368(lipea)
IPU: 108256376(lipe) 108272659
PHYP: 113532968(lipe) 113535092
AMEX: 103030794(lipe) 103036112
EEE: 113571934(lipe) 113578376
TRU: 101067661(lipe) 101070911
LCO: 104927333(lipe) 104928056
CGOB: 115015627 115021198(lipe)
ELY: 117263762(lipea) 117265772(lipeb)
PLEP: 121945131(lipeb) 121958297(lipea)
SLUC: 116042460(lipeb) 116047175(lipea)
ECRA: 117945621(lipeb) 117957028(lipea)
PFLV: 114556966(lipe) 114567920
GAT: 120811071(lipeb) 120826336(lipea)
PPUG: 119197023(lipeb) 119218887(lipea)
MSAM: 119897337(lipeb) 119907925(lipea)
CUD: 121513730(lipeb) 121524390(lipea)
MZE: 101473301 101485272(lipe)
ONL: 100534456(lipe) 100708083
OAU: 116309493(lipeb) 116318878(lipea)
OLA: 101157970(lipe) 101160745
OML: 112143493(lipeb) 112160867(lipea)
XMA: 102217205 102234893(lipe)
XCO: 114138258(lipe) 114156439
XHE: 116716805(lipe) 116731753
PRET: 103473021 103477914(lipe)
CVG: 107083574(lipe)
CTUL: 119771906(lipeb) 119781167(lipea)
NFU: 107379673(lipe) 107394884
KMR: 108229521(lipea) 108235517(lipeb)
ALIM: 106523738(lipe) 106530086
AOCE: 111564221(lipe) 111580224
CSEM: 103388799(lipe) 103393953
POV: 109633466(lipe) 109640422
LCF: 108880754(lipe) 108899366
SDU: 111230513 111236037(lipe)
SLAL: 111657262(lipe) 111661080
XGL: 120784156(lipeb) 120786574(lipea)
HCQ: 109513537 109531659(lipe)
BPEC: 110168856 110174235(lipe)
MALB: 109954692(lipe) 109958776
SFM: 108922191(lipe)
PKI: 111854848(tmem145)
AANG: 118229831(lipeb)
PSPA: 121299176(lipeb) 121304208
ARUT: 117434050(lipeb) 117969359
LCM: 102346636(LIPE)
BFO: 118424806
BBEL: 109479227
CIN: 100181083
SCLV: 120335513
APLC: 110988815
SKO: 100376662
DME: Dmel_CG11055(Hsl)
DER: 6547758
DSE: 6610042
DSI: Dsimw501_GD11499(Dsim_GD11499)
DAN: 6494614
DSR: 110179545
DPE: 6593171
DMN: 108160005
DWI: 6640914
DGR: 6568727
DAZ: 108616685
DNV: 108654876
DHE: 111593545
DVI: 6625071
CCAT: 101462188
BOD: 106621184
MDE: 101899540
SCAC: 106083183
LCQ: 111687523
ACOZ: 120948401
AARA: 120908555
AAG: 5566934
CPII: 120417328
AME: 413702
ACER: 107994522
BIM: 100748813
BBIF: 117209615
BVK: 117234575
BVAN: 117158600
BTER: 100646474
CCAL: 108623011
OBB: 114874463
MGEN: 117220220
NMEA: 116426143
CGIG: 122402372
SOC: 105198580
MPHA: 105835099
AEC: 105143573
ACEP: 105620501
PBAR: 105428403
VEM: 105562337
HST: 105188918
DQU: 106743086
FEX: 115237774
LHU: 105676433
PGC: 109856830
OBO: 105286291
PCF: 106788649
PFUC: 122514241
NVI: 100120573
CSOL: 105364358
TPRE: 106648819
MDL: 103568258
FAS: 105270162
DAM: 107041986
CCIN: 107264147
TCA: 664547
DPA: 109543096
ATD: 109602787
AGB: 108911225
LDC: 111506685
NVL: 108569775
APLN: 108745144
PPYR: 116182824
OTU: 111423682
BMOR: 101737209
BMAN: 114239644
MSEX: 115452516
BANY: 112051309
PMAC: 106708647
PPOT: 106104345
PXU: 106121800
PRAP: 110998313
ZCE: 119832926
HAW: 110384566
TNL: 113495550
PXY: 105392962
API: 100159927
DNX: 107171212
AGS: 114124269
RMD: 113549469
BTAB: 109032262
CLEC: 106668997
HHAL: 106688498
NLU: 111053191
FOC: 113207942
ZNE: 110826823
CSEC: 111861999
FCD: 110844206
DMK: 116918167
HAME: 121857466
HAZT: 108668222
VDE: 111243951
VJA: 111271304
DPTE: 113797455
CSCU: 111613708
PTEP: 107442104
SDM: 118189220
CEL: CELE_C46C11.1(hosl-1)
CBR: CBG14512
TSP: Tsp_02264
PCAN: 112567841
BGT: 106058325
GAE: 121386894
CRG: 105345018
MYI: 110445915
OBI: 106878881
OSN: 115211786
EGL: EGR_02179
NVE: 5511001
EPA: 110241065
ATEN: 116303467
AMIL: 114951856
PDAM: 113675352
SPIS: 111332161
DGT: 114524710
HMG: 100210426
AQU: 105314138
SPAR: SPRG_01501
 » show all
Reference
1  [PMID:10940339]
  Authors
Holm C, Osterlund T, Laurell H, Contreras JA.
  Title
Molecular mechanisms regulating hormone-sensitive lipase and lipolysis.
  Journal
Annu Rev Nutr 20:365-93 (2000)
DOI:10.1146/annurev.nutr.20.1.365
Reference
2  [PMID:7240206]
  Authors
Fredrikson G, Stralfors P, Nilsson NO, Belfrage P.
  Title
Hormone-sensitive lipase of rat adipose tissue. Purification and some properties.
  Journal
J Biol Chem 256:6311-20 (1981)
Reference
3  [PMID:14169138]
  Authors
VAUGHAN M, BERGER JE, STEINBERG D.
  Title
HORMONE-SENSITIVE LIPASE AND MONOGLYCERIDE LIPASE  ACTIVITIES IN ADIPOSE TISSUE.
  Journal
J Biol Chem 239:401-9 (1964)
Reference
4  [PMID:8912675]
  Authors
Osterlund T, Danielsson B, Degerman E, Contreras JA, Edgren G, Davis RC, Schotz MC, Holm C.
  Title
Domain-structure analysis of recombinant rat hormone-sensitive lipase.
  Journal
Biochem J 319 ( Pt 2):411-20 (1996)
DOI:10.1042/bj3190411
Reference
5  [PMID:3196730]
  Authors
Lee FT, Adams JB, Garton AJ, Yeaman SJ.
  Title
Hormone-sensitive lipase is involved in the hydrolysis of lipoidal derivatives of estrogens and other steroid hormones.
  Journal
Biochim Biophys Acta 963:258-64 (1988)
DOI:10.1016/0005-2760(88)90289-5
Reference
6  [PMID:9162045]
  Authors
Wei S, Lai K, Patel S, Piantedosi R, Shen H, Colantuoni V, Kraemer FB, Blaner WS.
  Title
Retinyl ester hydrolysis and retinol efflux from BFC-1beta adipocytes.
  Journal
J Biol Chem 272:14159-65 (1997)
DOI:10.1074/jbc.272.22.14159
Reference
7  [PMID:2794798]
  Authors
Tsujita T, Ninomiya H, Okuda H.
  Title
p-nitrophenyl butyrate hydrolyzing activity of hormone-sensitive lipase from bovine adipose tissue.
  Journal
J Lipid Res 30:997-1004 (1989)
Reference
8  [PMID:14725507]
  Authors
Yeaman SJ.
  Title
Hormone-sensitive lipase--new roles for an old enzyme.
  Journal
Biochem J 379:11-22 (2004)
DOI:10.1042/BJ20031811
Other DBs
ExplorEnz - The Enzyme Database: 3.1.1.79
IUBMB Enzyme Nomenclature: 3.1.1.79
ExPASy - ENZYME nomenclature database: 3.1.1.79
BRENDA, the Enzyme Database: 3.1.1.79

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