KEGG   ENZYME: 3.1.21.5Help
Entry
EC 3.1.21.5                 Enzyme                                 

Name
type III site-specific deoxyribonuclease;
type III restriction enzyme;
restriction-modification system
Class
Hydrolases;
Acting on ester bonds;
Endodeoxyribonucleases producing 5'-phosphomonoesters
BRITE hierarchy
Reaction(IUBMB)
Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates
Comment
This is a large group of enzymes which, together with those now listed as EC 3.1.21.3 (type 1 site-specific deoxyribonuclease) and EC 3.1.21.4 (type II site-specific deoxyribonuclease), were previously listed separately in sub-subclasses EC 3.1.23 and EC 3.1.24. They have an absolute requirement for ATP but do not hydrolyse it; S-adenosy-L-methionine stimulates the reaction, but is not absolutely required. They recognize specific, short DNA sequences and cleave a short distance away from the recognition sequence. These enzymes exist as complexes with enzymes of similar specificity listed under EC 2.1.1.72 [site-specific DNA-methyltransferase (adenine-specific)] or EC 2.1.1.73
History
EC 3.1.21.5 created 1984 from EC 3.1.23 and EC 3.1.24
Orthology
K01156  type III restriction enzyme
Genes
ESO: O3O_21090
ESM: O3M_04605
ESL: O3K_04560
ECW: EcE24377A_3325
ECC: c5372
ECQ: ECED1_2296
ECK: EC55989_3269
ELO: EC042_4599(pstIIR)
ELN: NRG857_21725
EAB: ECABU_c49390
ELC: i14_4892
ELD: i02_4892
ELF: LF82_720
STT: t0871
SEO: STM14_0418(res)
SEY: SL1344_0353(res)
SEJ: STMUK_0364(res)
SEB: STM474_0372(res)
SEF: UMN798_0391(res)
SENR: STMDT2_03541(res)
SEND: DT104_04021(res)
SPT: SPA2365(res)
SEK: SSPA2206
SEI: SPC_0369(res)
SEC: SCH_0399(res)
SHB: SU5_01052
SENS: Q786_01760
SED: SeD_A0390
SEG: SG0370(res)
SET: SEN0341(res)
SENA: AU38_01730
SENO: AU37_01725
SENV: AU39_01730
SENQ: AU40_01940
SENL: IY59_01775
SENB: BN855_3500(res)
SENE: IA1_01935
SBG: SBG_0313(res)
SBZ: A464_310
KPP: A79E_1792
KPNU: LI86_08965
EBF: D782_3961
YIN: CH53_1231
SMAR: SM39_2668
EAM: EAMY_0850(resB)
EAY: EAM_0862
XBV: XBW1_2424(res)
XPO: XPG1_0009 XPG1_0386(res)
PSI: S70_12515
HIT: NTHI1216(res)
HIE: R2846_1279(res)
HIZ: R2866_1344(res) R2866_1776(mod2)
HIK: HifGL_000720(res)
HIA: H733_1395
HDU: HD_1690
PMU: PM0699
MSU: MS1027
MHAT: B824_16120
MHAE: F382_02625
MHAM: J450_02100
MHAO: J451_02920
MVG: X874_9890
APL: APL_0812(res)
ASU: Asuc_0674
AAT: D11S_0130
AAN: D7S_02124
AACN: AANUM_2202
BTO: WQG_11510
BTRE: F542_10540
BTRH: F543_11940
BTRA: F544_11890
XCC: XCC1067
XAX: XACM_1133
XOR: XOC_2322
PAEG: AI22_08685
PEN: PSEEN0754
PSIL: PMA3_26815
ACX: Achr_330
PALI: A3K91_0531
MCT: MCR_0361
SWD: Swoo_0304
PPHE: PP2015_280
AMAL: I607_02945
PIN: Ping_2486
CJA: CJA_1582(res) CJA_3651
LPO: LPO_0165
LPP: lpp0198
LHA: LHA_2865
FTU: FTT_1579c
FTF: FTF1579c
FTT: FTV_1507
FTG: FTU_1592
FTL: FTL_0528
FTH: FTH_0531(res)
FTA: FTA_0560
FTS: F92_02885
FTI: FTS_0532(res)
FTC: DA46_158
FTV: CH67_838
FTZ: CH68_571
FTN: FTN_1487
FTX: AW25_514
FPH: Fphi_1885
FPI: BF30_147
FPX: KU46_1405
FPJ: LA02_1618
NOC: Noc_2927
NHL: Nhal_3378
TOL: TOL_0429
EBH: BSEPE_0561(res)
NME: NMB1260(res)
NMN: NMCC_1289
BMA: BMA2673
BMAL: DM55_1194
BMAE: DM78_3157
BMAQ: DM76_1170
BMAI: DM57_1507
BMAF: DM51_2304
BMAZ: BM44_620
BMAB: BM45_74
BPS: BPSL0043
BPSE: BDL_1901
BPSM: BBQ_3361
BPSU: BBN_3482
BPSD: BBX_292
BPK: BBK_1389
BPSH: DR55_1029
BPSA: BBU_2060
BPSO: X996_635
BTE: BTH_I0042
BTQ: BTQ_61
BTJ: BTJ_2384
BTZ: BTL_326
BTD: BTI_84
BTHM: BTRA_501
BTHA: DR62_1231
BTHL: BG87_501
BOC: BG90_1535
BVE: AK36_728
BCN: Bcen_0043
BCJ: BCAL3493
BCEN: DM39_3302
BCEW: DM40_802
BCEO: I35_3273
BAM: Bamb_0019
BMU: Bmul_0028
BMJ: BMULJ_00042(res)
BMK: DM80_1662
BMUL: NP80_161
BCT: GEM_0014
BCED: DM42_1752
BDL: AK34_3050
BCON: NL30_19360
BLAT: WK25_02535
BTEI: WS51_10985
BSEM: WJ12_00190
BPSL: WS57_13140
BMEC: WJ16_00150
BSTG: WT74_00280
BGU: KS03_929
BGO: BM43_1417
BUK: MYA_0024
BUL: BW21_156
BPE: BP3117
BPC: BPTD_3080
BPET: B1917_0705
BPEU: Q425_30950
BBR: BB0913
BPT: Bpet4237(res)
AAV: Aave_0162
DAC: Daci_4204
CTES: O987_04505
HYB: Q5W_06580
MMS: mma_2791
LCH: Lcho_4167
TIN: Tint_1530
RGE: RGE_16330
NEU: NE2306
EBA: ebA2417
DSU: Dsui_3355
AZO: azo2682
BPRC: D521_2087
HPJ: jhp_1297(res_1) jhp_1410(res_2)
HPP: HPP12_0599(res-1) HPP12_1496(res-4)
HPL: HPB8_1553(res_1) HPB8_9(res)
HEF: HPF16_1297(res_1) HPF16_1416(res-4)
HPF: HPF30_1274(res_1) HPF30_1392(res-4)
HEQ: HPF32_1292(res_1) HPF32_1405(res-4) HPF32_1406(res-4)
HEX: HPF57_1321(res_1) HPF57_1433(res-4)
HPD: KHP_1257(res_1) KHP_1373(res)
HCM: HCD_04565
CJS: CJS3_0619
CJQ: UC78_0278
CJR: CJE0732
CHYO: CHH_1056
CCUN: CCUN_1816
CLX: CLAN_1189
GME: Gmet_0675
GUR: Gura_4389
PCA: Pcar_2729
PPD: Ppro_0236
DEU: DBW_3089
DDE: Dde_1742
DAS: Daes_1669
PPRF: DPRO_1164
SAT: SYN_01824
SFU: Sfum_2855
DBR: Deba_1598
MES: Meso_0544
SMD: Smed_5437
BRA: BRADO1376
BRAD: BF49_0445
RPE: RPE_0562
RPT: Rpal_3802
BTR: BT_1179 BT_1739(res1) BT_1930(res2)
AZC: AZC_3259
MSL: Msil_1948
RCP: RCAP_rcc01261(res)
DSH: Dshi_1457
MALG: MALG_01484
HBA: Hbal_1634
SPHM: G432_08450
STAX: MC45_14350
SPKC: KC8_09765
SPMI: K663_22638
SINB: SIDU_10785
SPHT: K426_27140
AAY: WYH_00580
GXL: H845_990
RRU: Rru_A0242
RRF: F11_01225
RCE: RC1_0095
TMO: TMO_c0629
MGM: Mmc1_0790
AFR: AFE_3191
BSX: C663_3934(res)
BCA: BCE_1019
BNC: BCN_5051
BAG: Bcoa_0568
BCOA: BF29_1257
AAMY: GFC30_1958
SSCH: LH95_00195
MCL: MCCL_0235(hsdR)
LMOM: IJ09_04795
AAC: Aaci_3087
LLC: LACR_2573
LLR: llh_14205
SAGM: BSA_2940
SAGR: SAIL_20130
STC: str0884(sthIR)
STL: stu0884(sthIR)
STU: STH8232_1075(sthIR)
SSI: SSU0642
SUP: YYK_03035
SSUI: T15_1304(res)
SUB: SUB1188
SDA: GGS_0288
SGG: SGGBAA2069_c10290(sthIR)
SMB: smi_1247
SOR: SOR_0900
SMN: SMA_0979(hsdR)
SIE: SCIM_0692
SCG: SCI_1423
SCON: SCRE_1380
SCOS: SCR2_1380
STRN: SNAG_0912
LJF: FI9785_308(res)
LJH: LJP_1436c
LSL: LSL_1759
LSI: HN6_01543
LBR: LVIS_1621
LCA: LSEI_0585
LGA: LGAS_1463
LRT: LRI_0541
LHE: lhv_0028
LFE: LAF_1042
LFR: LC40_0680
LRH: LGG_01027(res)
LRG: LRHM_0982
LKE: WANG_1120
LRM: LRC_08820
ENE: ENT_29680
EFM: M7W_760
CPF: CPF_0362
CPAS: Clopa_2249
CSQ: CSCA_1133
AOE: Clos_1318
CTH: Cthe_0518
RCH: RUM_10360
FPR: FP2_12710
CPRO: CPRO_18560
SWO: Swol_1547
DAU: Daud_0199
DEC: DCF50_p51
HMO: HM1_3003
OVA: OBV_09170(res)
SAY: TPY_0431
THX: Thet_1110
TIT: Thit_1898
TKI: TKV_c14520(res)
TOC: Toce_1948
APR: Apre_0347
VPR: Vpar_0449
AIN: Acin_0681
MPU: MYPU_3950(MYPU_3950) MYPU_4790(MYPU_4790)
MPE: MYPE4800(MYPE4800)
MMO: MMOB4610 MMOB4620(ssl2)
MHY: mhp331
MHJ: MHJ_0309
MAA: MAG1520
MAL: MAGa1560
MHO: MHO_0120
MHOM: MLBD4_00080(res)
MHR: MHR_0174
MHH: MYM_0186
MHM: SRH_01090
MHS: MOS_201
MHV: Q453_0199
MFR: MFE_00300(ssl2)
MBH: MMB_0157
MBI: Mbov_0165
MBC: MYB_02400
MGJ: MGM1_1970
MARG: MARG145_0342(res)
UUR: UU476
UPA: UPA3_0494
MMM: W7S_10060
MABB: MASS_2539
CGJ: AR0_10555
CJK: jk0525(resA) jk1240(resB)
CUR: cu1795
CUA: CU7111_1378(resA)
CRD: CRES_0908
CUJ: CUL131002_0983(res)
CPHO: CPHO_08975
CAQU: CAQU_03255
CMIN: NCTC10288_01746(resA)
ACH: Achl_4044
PAC: PPA1612
CACN: RN83_07095
PFRE: RM25_1602
MPH: MLP_35810(res)
NCA: Noca_1086
ACAD: UA74_05030
AHE: Arch_0781
BLL: BLJ_0935
BAST: BAST_0040
AFO: Afer_1393
ELE: Elen_0929
GPA: GPA_35200
CYA: CYA_1293
TEL: tll1480
PRC: EW14_1405
MAR: MAE_44800
MPK: VL20_2525
DET: DET1114
DMC: btf_159
DMD: dcmb_73
DMG: GY50_1040
TAQ: TO73_1566
MRB: Mrub_0579
AMU: Amuc_1522
PSL: Psta_0261
TTF: THTE_3471
EMI: Emin_0226
ETI: RSTT_382(res)
FNU: FN0417
LBA: Lebu_0154
SMF: Smon_1418
TLI: Tlie_1266
SBR: SY1_08380
PGN: PGN_0862
PMUC: ING2E5A_2064(res)
PSAC: PSM36_3490
FLN: FLA_4987
DFE: Dfer_5226
FPS: FP2167
RAG: B739_2007
CHZ: CHSO_1628
WIN: WPG_2221
CTE: CT0911(res) CT1730
CLI: Clim_1468
KOL: Kole_0061
NDE: NIDE0709
CTHI: THC_0379
MST: Msp_0473
MEB: Abm4_0084
MCUB: MCBB_0204
TVO: TVG1539639(TVG1539639) TVG1552703(TVG1552703)
CDIV: CPM_0054
METM: MSMTP_1487
MCJ: MCON_2054
MEMA: MMAB1_1228
RCI: LRC80
 » show all
Taxonomy
Reference
1  [PMID:2159140]
  Authors
Roberts RJ.
  Title
Restriction enzymes and their isoschizomers.
  Journal
Nucleic Acids Res 18 Suppl:2331-65 (1990)
Other DBs
ExplorEnz - The Enzyme Database: 3.1.21.5
IUBMB Enzyme Nomenclature: 3.1.21.5
ExPASy - ENZYME nomenclature database: 3.1.21.5
BRENDA, the Enzyme Database: 3.1.21.5
CAS: 9075-08-5

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