KEGG   ENZYME: 3.1.3.77Help
Entry
EC 3.1.3.77                 Enzyme                                 

Name
acireductone synthase;
E1;
E-1 enolase-phosphatase;
5-(methylthio)-2,3-dioxopentyl-phosphate phosphohydrolase (isomerizing)
Class
Hydrolases;
Acting on ester bonds;
Phosphoric-monoester hydrolases
BRITE hierarchy
Sysname
5-(methylsulfanyl)-2,3-dioxopentyl-phosphate phosphohydrolase (isomerizing)
Reaction(IUBMB)
5-(methylsulfanyl)-2,3-dioxopentyl phosphate + H2O = 1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + phosphate (overall reaction) [RN:R07395];
(1a) 5-(methylsulfanyl)-2,3-dioxopentyl phosphate = 2-hydroxy-5-(methylsulfanyl)-3-oxopent-1-enyl phosphate (probably spontaneous) [RN:R07393];
(1b) 2-hydroxy-5-(methylsulfanyl)-3-oxopent-1-enyl phosphate + H2O = 1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + phosphate [RN:R07394]
Reaction(KEGG)
Substrate
5-(methylsulfanyl)-2,3-dioxopentyl phosphate [CPD:C15650];
H2O [CPD:C00001];
2-hydroxy-5-(methylsulfanyl)-3-oxopent-1-enyl phosphate [CPD:C15651]
Product
1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one [CPD:C15606];
phosphate [CPD:C00009];
2-hydroxy-5-(methylsulfanyl)-3-oxopent-1-enyl phosphate [CPD:C15651]
Comment
This bifunctional enzyme first enolizes the substrate to form the intermediate 2-hydroxy-5-(methylsulfanyl)-3-oxopent-1-enyl phosphate, which is then dephosphorylated to form the acireductone 1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one [2]. The acireductone represents a branch point in the methione-salvage pathway as it is used in the formation of formate, CO and 3-(methylsulfanyl)propanoate by EC 1.13.11.53 [acireductone dioxygenase (Ni2+-requiring)] and of formate and 4-(methylsulfanyl)-2-oxobutanoate either by a spontaneous reaction under aerobic conditions or by EC 1.13.11.54 {acireductone dioxygenase [iron(II)-requiring]} [1,2].
History
EC 3.1.3.77 created 2006
Pathway
ec00270  Cysteine and methionine metabolism
ec01100  Metabolic pathways
Orthology
K09880  enolase-phosphatase E1
K16054  methylthioribulose 1-phosphate dehydratase / enolase-phosphatase E1
Genes
HSA: 58478(ENOPH1)
PTR: 461214(ENOPH1)
PPS: 100974648(ENOPH1)
GGO: 101142610(ENOPH1)
PON: 100442505(ENOPH1)
NLE: 100587842(ENOPH1)
MCC: 699388
MCF: 102133226
CSAB: 103235888(ENOPH1)
RRO: 104658818(ENOPH1)
RBB: 108516978(ENOPH1)
CJC: 100413041(ENOPH1)
SBQ: 101028726 101036278(ENOPH1)
MMU: 67870(Enoph1)
RNO: 305177(Enoph1)
CGE: 100767312(Enoph1)
NGI: 103744997(Enoph1)
HGL: 101721469(Enoph1)
CCAN: 109698152(Enoph1)
OCU: 100346300(ENOPH1)
TUP: 102474966 102481555(ENOPH1)
CFA: 478452(ENOPH1)
AML: 100466886(ENOPH1)
UMR: 103665357(ENOPH1)
ORO: 101377341(ENOPH1)
FCA: 101087835(ENOPH1)
PTG: 102970083(ENOPH1)
AJU: 106971287(ENOPH1)
BTA: 525563(ENOPH1)
BOM: 102279202(ENOPH1)
BIU: 109560627(ENOPH1)
PHD: 102326015(ENOPH1)
CHX: 102174551(ENOPH1)
OAS: 101114870(ENOPH1)
SSC: 100511394(ENOPH1)
CFR: 102517973(ENOPH1)
CDK: 105091871(ENOPH1)
BACU: 103017566(ENOPH1)
LVE: 103088594(ENOPH1)
OOR: 101287181(ENOPH1)
ECB: 100060891(ENOPH1)
EPZ: 103554636(ENOPH1)
EAI: 106846433(ENOPH1)
MYB: 102255328(ENOPH1)
MYD: 102774263(ENOPH1)
HAI: 109387936(ENOPH1)
RSS: 109441006 109447225(ENOPH1)
PALE: 102892324(ENOPH1)
LAV: 100655843(ENOPH1)
TMU: 101357062
MDO: 100010409(ENOPH1)
SHR: 100928740(ENOPH1)
OAA: 100082028(ENOPH1)
GGA: 422600(ENOPH1)
MGP: 100544131(ENOPH1)
CJO: 107313415(ENOPH1)
APLA: 101797287(ENOPH1)
ACYG: 106041863(ENOPH1)
TGU: 100221564(ENOPH1)
GFR: 102041605(ENOPH1)
FAB: 101813026(ENOPH1)
PHI: 102108958(ENOPH1)
PMAJ: 107203310(ENOPH1)
CCW: 104697323(ENOPH1)
FPG: 101920749(ENOPH1)
FCH: 102058814(ENOPH1)
CLV: 102092992(ENOPH1)
EGZ: 104133991(ENOPH1)
AAM: 106499885(ENOPH1)
ASN: 102370991(ENOPH1)
AMJ: 102571584(ENOPH1)
PSS: 102451220(ENOPH1)
CMY: 102941800(ENOPH1)
CPIC: 101947284(ENOPH1)
ACS: 100563803(enoph1)
PVT: 110083350(ENOPH1)
PBI: 103053372(ENOPH1)
GJA: 107113912(ENOPH1)
XLA: 734408(enoph1.S)
XTR: 733933(enoph1)
NPR: 108789073(ENOPH1)
DRE: 431773(enoph1)
SGH: 107558259(enoph1) 107573845
IPU: 108276834(enoph1)
AMEX: 103024728(enoph1)
TRU: 101061561(enoph1) 101070871
LCO: 104938133(enoph1)
NCC: 104960870(enoph1)
MZE: 101466298(enoph1)
OLA: 101171682(enoph1)
XMA: 102223202(enoph1)
PRET: 103473268(enoph1)
NFU: 107374809(enoph1)
CSEM: 103389919(enoph1)
LCF: 108895475(enoph1)
HCQ: 109529132(enoph1)
BPEC: 110157539(enoph1)
SASA: 100195051(enoph) 106568328
ELS: 105014810(enoph1)
SFM: 108929930(enoph1)
LCM: 102353763(ENOPH1)
CMK: 103175781(enoph1)
CIN: 100175859
SPU: 589774
APLC: 110987133
SKO: 100369438
DME: Dmel_CG12173(CG12173)
DSI: Dsimw501_GD19634(Dsim_GD19634)
MDE: 101890600
AGA: AgaP_AGAP003331(ENOPH_ANOGA)
AME: 411809
BIM: 100749373
BTER: 100645779
SOC: 105195665
AEC: 105144576
ACEP: 105622504
PBAR: 105431756
HST: 105192493
CFO: 105252042
LHU: 105668805
PGC: 109852040
NVI: 100117336
TCA: 664509
DPA: 109536834
NVL: 108562678
PMAC: 106721644
API: 100167644
DNX: 107163794
ZNE: 110828690
FCD: 110844631
TUT: 107365905
CEL: CELE_F58H1.3(F58H1.3)
CBR: CBG23332
CRG: 105329340
MYI: 110466335
LAK: 106164209
EPA: 110238877
ADF: 107334844
HMG: 101235186
AQU: 100634727
ATH: AT5G53850
CRB: 17876727
THJ: 104799927
CIT: 102631398
VRA: 106755004
VAR: 108334227
CAM: 101495055
LJA: Lj0g3v0347379.2(Lj0g3v0347379.2) Lj3g3v0429670.1(Lj3g3v0429670.1)
ADU: 107462602
AIP: 107613086
FVE: 101301667
PPER: 18778522
PMUM: 103326196
PAVI: 110773320
PXB: 103947667
RCU: 8265316
POP: 18095573
INI: 109178424
SOE: 110783623
DOSA: Os01t0101200-01(Os01g0101200) Os11t0484000-01(Os11g0484000)
BDI: 100828599
ATS: 109757853(LOC109757853)
SBI: 8155324
ZMA: 100194257(IDP664) 100282341(IDP388)
SITA: 101776717
PDA: 103696410
EGU: 105034201
ATR: 18448898
MNG: MNEG_7901
APRO: F751_1034
SCE: YEL038W(UTR4)
ERC: Ecym_3571
KMX: KLMA_10705(UTR4)
NCS: NCAS_0G04130(NCAS0G04130)
NDI: NDAI_0G00320(NDAI0G00320)
TPF: TPHA_0F03320(TPHA0F03320)
TBL: TBLA_0D03890(TBLA0D03890)
TDL: TDEL_0C02580(TDEL0C02580)
KAF: KAFR_0E00520(KAFR0E00520)
CAL: CAALFM_C201860CA(CaO19.1495)
CAUR: QG37_06680
SLB: AWJ20_5096(UTR4)
NCR: NCU06228
NTE: NEUTE1DRAFT122023(NEUTE1DRAFT_122023)
MGR: MGG_10183
SSCK: SPSK_02744
MAW: MAC_01899
MAJ: MAA_00599
CMT: CCM_05841
MBE: MBM_01018
PTE: PTT_16865
DFA: DFA_07239
SMIN: v1.2.009351.t1(symbB.v1.2.009351.t1)
ENC: ECL_03087
ECLO: ENC_21180
EEC: EcWSU1_01188(mtnC)
ECLX: LI66_05765
ECLY: LI62_06665
ECLZ: LI64_06265
ECAN: CWI88_16355(mtnC)
ESA: ESA_02725
CSK: ES15_2795(mtnC)
CTU: CTU_12370(mtnC)
KPN: KPN_00644
KPU: KP1_1595(mtnC)
KPP: A79E_3596
KPT: VK055_1889(mtnC)
KPE: KPK_3930(mtnC)
KPR: KPR_3932(mtnC)
KPJ: N559_3681
KPNU: LI86_18880
KPNK: BN49_1702(mtnC)
KVA: Kvar_3723
KOX: KOX_14115
KOE: A225_1641
EAE: EAE_13735
EAR: CCG31224
KQV: B8P98_20465(mtnC)
CKO: CKO_03972
RAO: DSD31_18390(mtnC)
LEI: C2U54_10925(mtnC)
LEH: C3F35_05545(mtnC)
LNI: CWR52_06325(mtnC)
LEW: DAI21_20790(mtnC)
EBC: C2U52_29115(mtnC)
YPS: YPTB0875
YPO: BZ17_1671(mtnC)
YPY: YPK_3320
YPB: YPTS_0916
YPQ: DJ40_1483(mtnC)
YPU: BZ21_120(mtnC)
YPR: BZ20_1172(mtnC)
YPC: BZ23_397(mtnC)
YPF: BZ19_233(mtnC)
YEN: YE3232(mntC)
YEY: Y11_21601
YEL: LC20_01263(mtnC)
YEW: CH47_2600(mtnC)
YET: CH48_2606(mtnC)
YAL: AT01_1543(mtnC)
YFR: AW19_2211(mtnC)
YIN: CH53_2776(mtnC)
YKR: CH54_3575(mtnC)
YRO: CH64_3496(mtnC)
YMA: DA391_17340(mtnC)
SPE: Spro_0943
SRL: SOD_c07980(mtnC)
SPLY: Q5A_004245(dehH2)
SMAF: D781_0893
SMAR: SM39_0248(masA)
SMAC: SMDB11_0200(masA)
SERF: L085_24085
SERM: CLM71_04335(mtnC)
RAA: Q7S_04480
ECA: ECA3486(masA)
PATR: EV46_17260
PATO: GZ59_35000(masA)
PCT: PC1_3302
PEC: W5S_3592
SOD: Sant_3602(masA)
DDD: Dda3937_00922(masA)
DDQ: DDI_3216
DFN: CVE23_17365(mtnC)
ETA: ETA_26080(masA)
EPY: EpC_27300(mtnC)
EPR: EPYR_02967(masA)
EAM: EAMY_0874(masA)
EAY: EAM_0886(MasA)
EBI: EbC_08700
ERJ: EJP617_20040(mtnC)
EGE: EM595_0865(masA)
PAM: PANA_0872
PAJ: PAJ_0207
PVA: Pvag_0265(masA)
PAGG: AL522_08280(mtnC)
PSTW: DSJ_19575
PGZ: C2E15_05105(mtnC)
PCD: C2E16_04995(mtnC)
XFA: XF_2211
XFT: PD_1260(masA)
XCC: XCC1818
XCB: XC_2371
XCP: XCR_2069
XCV: XCV1884
XAX: XACM_1864
XAC: XAC1838
XOO: XOO2137
XOM: XOO2006(XOO2006)
XOR: XOC_2226
XAL: XALC_1320
XPH: XppCFBP6546_19990(XppCFBP6546P_19990)
XVA: C7V42_12780(mtnC)
SML: Smlt2187(masA)
SMT: Smal_1780
SMZ: SMD_1970(masA)
SACZ: AOT14_20660(masA)
STEN: CCR98_09570(mtnC)
STEM: CLM74_10005(mtnC)
PSUW: WQ53_14310
PSD: DSC_09805
LAB: LA76x_3214(mtnC)
LAQ: GLA29479_3660(mtnC)
LCP: LC55x_3260(mtnC)
LGU: LG3211_1959(mtnC)
LEZ: GLE_1910(mtnC)
LEM: LEN_3080
LYT: DWG18_07975(mtnC)
LUM: CNR27_08575(mtnC)
LUE: DCD74_05250(mtnC)
DKO: I596_1902
XBA: C7S18_13650(mtnC)
PAE: PA1685(masA)
PAEV: N297_1731(mtnC)
PAEI: N296_1731(mtnC)
PAU: PA14_42720(masA)
PAP: PSPA7_3587(mtnC)
PAG: PLES_36421(masA)
PAF: PAM18_3362(masA)
PNC: NCGM2_2583(masA)
PAEB: NCGM1900_4882(masA)
PAEP: PA1S_17585
PAEM: U769_17305
PAEL: T223_18640
PAEU: BN889_01806(masA)
PAEG: AI22_16400
PAEC: M802_1728(mtnC)
PAEO: M801_1730(mtnC)
PRE: PCA10_37320(mtnC)
PCQ: PcP3B5_39740(mtnC)
PSB: Psyr_1857
PSYR: N018_08660
PAMG: BKM19_011595(mtnC)
PAVL: BKM03_19315(mtnC)
PMAN: OU5_5345(mtnC)
PSTT: CH92_08470
PPUU: PputUW4_03697(mtnC)
PKC: PKB_1890(mtnC)
PSET: THL1_3690
SON: SO_0084(mtnC)
SDN: Sden_0077
SFR: Sfri_3966
SAZ: Sama_0074
SBL: Sbal_4264
SLO: Shew_3771
SSE: Ssed_4454
SPL: Spea_4180
SHL: Shal_0067
SWD: Swoo_4855
SWP: swp_0123
SVO: SVI_4285
SHF: CEQ32_16685(mtnC)
SPSW: Sps_05603
SBJ: CF168_00480(mtnC)
SMAV: CFF01_18680(mtnC)
SHEW: CKQ84_08650(mtnC)
PSM: PSM_A3023
PSEO: OM33_15845
PPHE: PP2015_165
PPIS: B1L02_17560(mtnC)
PEA: PESP_a3748(mtnC)
PSPO: PSPO_b0242(mtnC)
PART: PARC_a0118(mtnC)
PTU: PTUN_a0117(mtnC)
PTD: PTET_a3413(mtnC)
PSEN: PNC201_16980(mtnC)
MAQ: Maqu_0909
MHC: MARHY2379
MAD: HP15_1262
FBL: Fbal_3776
MCA: MCA0797
MAH: MEALZ_2473(mtnC)
MPSY: CEK71_20325(mtnC)
PSAL: PSLF89_563
NHL: Nhal_0743
NWA: Nwat_0696
TEE: Tel_07805
NTT: TAO_1469
TGR: Tgr7_2625
TKM: TK90_1789
TVR: TVD_04610
HCH: HCH_01846
HAM: HALO0805
HHH: CLM76_08200(mtnC)
HAF: C8233_13765(mtnC)
ABO: ABO_1447
ADI: B5T_02225
AXE: P40_10725
KAK: Kalk_01715(mtnC)
TOL: TOL_3010
BSAN: CHH28_19480(mtnC)
SOK: D0B54_21100(mtnC)
SLIM: SCL_0168
SVA: SVA_2385
SALN: SALB1_1399
CCX: COCOR_07002(masA)
SCL: sce1551(masA)
BSTO: C0V70_15960(mtnC)
PLA: Plav_1854
BBT: BBta_2512
XAU: Xaut_2913
MSC: BN69_2606
MTW: CQW49_20655(mtnC)
SPHI: TS85_07960
SSAN: NX02_24540
SPHC: CVN68_01535(mtnC)
SPHT: K426_21269
GOX: GOX1244
GOH: B932_0887
GOY: GLS_c13310(mtnC)
ACR: Acry_0019
AMV: ACMV_00190(mtnC)
GDI: GDI0587
GDJ: Gdia_1413
GXY: GLX_12660
GXL: H845_2504
KEU: S101446_01812(mtnC)
APK: APA386B_2241(masA)
ASZ: ASN_416(masA)
APOM: CPF11_03785(mtnC)
ATO: CIW82_10485(mtnC)
ACET: DS739_03890(mtnC)
ROS: CTJ15_05710(mtnC)
TMO: TMO_a0145(mtnC)
NFA: NFA_45460
NNO: NONO_c39560(mtnC)
NSR: NS506_04592(mtnC)
NTP: CRH09_18165(mtnC)
SMA: SAVERM_6660(masA)
SCT: SCAT_3259(mtnC)
SRW: TUE45_02090(mtnC)
SLE: sle_00950(sle_00950)
SFK: KY5_1467
MTS: MTES_1966
HUM: DVJ78_17130(mtnC)
FAL: FRAAL1409(masA)
SEN: SACE_3607(masA)
AOI: AORI_2692(mtnC)
AJA: AJAP_25360(mtnC)
AMI: Amir_0985
APRE: CNX65_04950(mtnC)
SESP: BN6_11420(mtnC)
KAL: KALB_7735
ACTI: UA75_13685
ACAD: UA74_13590
AHG: AHOG_12820(mtnC)
ACTA: C1701_24870(mtnC)
ALL: CRK55074
AYM: YM304_28020(mtnC)
SYW: SYNW1964(masA)
SYC: syc2102_c(masA)
SYR: SynRCC307_0448(masA)
SYX: SynWH7803_0537(masA)
SYND: KR52_14475
SYH: Syncc8109_0536(masA)
SYNW: SynWH8103_02246(mtnC)
CYI: CBM981_1747(mtnC)
HHG: XM38_027820(mtnC)
PMT: PMT_0184
CYL: AA637_01925(mtnC)
GLJ: GKIL_3118(mtnC)
CTHE: Chro_0354
PSL: Psta_3141
LIL: LA_0929
LIE: LIF_A0755
LIC: LIC_12718
LIS: LIL_12808
LBJ: LBJ_2207
LBL: LBL_2200
LBF: LBF_2858
LST: LSS_18818
LMAY: DPV73_17670(mtnC)
SGN: SGRA_2507(mtnC)
FLU: CHH17_01685(mtnC)
AAE: aq_1977(masA)
HTH: HTH_1579
SAF: SULAZ_1667(mtnC)
PMX: PERMA_0829(mtnC)
CDIV: CPM_1788
 » show all
Taxonomy
Reference
1  [PMID:8227039]
  Authors
Myers RW, Wray JW, Fish S, Abeles RH.
  Title
Purification and characterization of an enzyme involved in oxidative carbon-carbon bond cleavage reactions in the methionine salvage pathway of Klebsiella pneumoniae.
  Journal
J Biol Chem 268:24785-91 (1993)
Reference
2  [PMID:7852397]
  Authors
Wray JW, Abeles RH.
  Title
The methionine salvage pathway in Klebsiella pneumoniae and rat liver. Identification and characterization of two novel dioxygenases.
  Journal
J Biol Chem 270:3147-53 (1995)
DOI:10.1074/jbc.270.7.3147
Reference
3  [PMID:15843022]
  Authors
Wang H, Pang H, Bartlam M, Rao Z.
  Title
Crystal structure of human E1 enzyme and its complex with a substrate analog reveals the mechanism of its phosphatase/enolase activity.
  Journal
J Mol Biol 348:917-26 (2005)
DOI:10.1016/j.jmb.2005.01.072
  Sequence
[hsa:58478]
Other DBs
ExplorEnz - The Enzyme Database: 3.1.3.77
IUBMB Enzyme Nomenclature: 3.1.3.77
ExPASy - ENZYME nomenclature database: 3.1.3.77
BRENDA, the Enzyme Database: 3.1.3.77

DBGET integrated database retrieval system