KEGG ENZYME: 3.1.4.50

ENZYME: 3.1.4.50

Entry

EC 3.1.4.50 Enzyme

Name

glycosylphosphatidylinositol phospholipase D;
GPI-PLD;
glycoprotein phospholipase D;
phosphatidylinositol phospholipase D;
phosphatidylinositol-specific phospholipase D

Class

Hydrolases;
Acting on ester bonds;
Phosphoric-diester hydrolases

Sysname

glycoprotein-phosphatidylinositol phosphatidohydrolase

Reaction(IUBMB)

6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + H2O = 6-(alpha-D-glucosaminyl)-1D-myo-inositol + 3-sn-phosphatidate [RN:R06623]

Reaction(KEGG)

R06623(G);
(other) R04149 R07398

Substrate

6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol [CPD:C04248];
H2O [CPD:C00001]

Product

6-(alpha-D-glucosaminyl)-1D-myo-inositol [CPD:C15658];
3-sn-phosphatidate [CPD:C00416]

Comment

This enzyme is also active when O-4 of the glucosamine is substituted by carrying the oligosaccharide that can link a protein to the structure. It therefore cleaves proteins from the lipid part of the glycosylphosphatidylinositol (GPI) anchors, but does so by hydrolysis, whereas glycosylphosphatidylinositol diacylglycerol-lyase (EC 4.6.1.14) does so by elimination. It acts on plasma membranes only after solubilization of the substrate with detergents or solvents, but it may act on intracellular membranes.

History

EC 3.1.4.50 created 1990, modified 2002

Pathway

ec00563

Glycosylphosphatidylinositol (GPI)-anchor biosynthesis

ec01100

Metabolic pathways

Orthology

K01127

glycosylphosphatidylinositol phospholipase D

Genes

HSA:	2822(GPLD1)

PTR:	462473(GPLD1)

PPS:	100973223(GPLD1)

GGO:	101146393(GPLD1)

PON:	100455517(GPLD1)

NLE:	100598329(GPLD1)

MCC:	707782(GPLD1)

MCF:	102125729(GPLD1)

CSAB:

103222053(GPLD1)

RRO:	104663891(GPLD1)

RBB:	108533667(GPLD1)

CJC:	100385911(GPLD1)

SBQ:	101035773(GPLD1)

MMU:	14756(Gpld1)

RNO:	291132(Gpld1)

CGE:	100759513(Gpld1)

NGI:	103745185(Gpld1)

HGL:	101724062(Gpld1)

CCAN:

109688371(Gpld1)

OCU:	100351916(GPLD1)

TUP:	102501907(GPLD1)

CFA:	478735(GPLD1)

AML:	100484842(GPLD1)

UMR:	103665176(GPLD1)

ORO:	101370230(GPLD1)

FCA:	101088486(GPLD1)

PTG:	102964289(GPLD1)

AJU:	106968449(GPLD1)

BTA:	287025(GPLD1)

BOM:	102286788(GPLD1)

BIU:	109577315(GPLD1)

PHD:	102336002(GPLD1)

CHX:	102169718(GPLD1)

OAS:	101119000(GPLD1)

SSC:	100157497(GPLD1)

CFR:	102518271(GPLD1)

CDK:	105089129(GPLD1)

BACU:

103003582(GPLD1)

LVE:	103088629(GPLD1)

OOR:	101288738(GPLD1)

ECB:	100067517(GPLD1)

EPZ:	103546694(GPLD1)

EAI:	106822357(GPLD1)

MYB:	102260484(GPLD1)

MYD:	102753404(GPLD1)

HAI:	109396397(GPLD1)

RSS:	109437528(GPLD1)

PALE:

102896882(GPLD1)

LAV:	100677378(GPLD1)

TMU:

101344243

MDO:	100024296(GPLD1)

SHR:	100931811(GPLD1)

OAA:	100092985(GPLD1)

GGA:	420819(GPLD1)

MGP:	100546397(GPLD1)

CJO:	107309738(GPLD1)

APLA:

101792700(GPLD1)

ACYG:

106038351(GPLD1)

TGU:	100230775(GPLD1)

GFR:	102032214(GPLD1)

FAB:	101816045(GPLD1)

PHI:	102114237(GPLD1)

PMAJ:

107200258(GPLD1)

CCAE:

111923724(GPLD1)

CCW:	104691444(GPLD1)

FPG:	101912228(GPLD1)

FCH:	102052413(GPLD1)

CLV:	102090900(GPLD1)

EGZ:	104125477(GPLD1)

AAM:	106483272(GPLD1)

ASN:	102375814(GPLD1)

AMJ:	102574726(GPLD1)

PSS:	102448848(GPLD1)

CMY:	102944962(GPLD1)

CPIC:

101945760(GPLD1)

ACS:	100567850(gpld1)

PVT:	110072878(GPLD1)

PBI:	103063314(GPLD1)

GJA:	107113400(GPLD1)

XLA:

108718535

NPR:	108792898(GPLD1)

LCM:	102345190(GPLD1)

BFO:	BRAFLDRAFT_241888

SPU:

590298

APLC:

110975439

SKO:

102801774

CRG:	105347431 105347449 105347450

MYI:

110456981

LAK:

106165615

NVE:	NEMVE_v1g115107

EPA:

110241955

ADF:

107355065

SCE:

YJL132W

KLA:	KLLA0_E15401g

KMX:	KLMA_60039

LTH:	KLTH0F05830g

ZRO:	ZYRO0D03256g

CGR:	CAGL0L05720g

NCS:	NCAS_0A09790(NCAS0A09790)

NDI:	NDAI_0G06190(NDAI0G06190)

TBL:	TBLA_0D05660(TBLA0D05660)

TDL:	TDEL_0H01030(TDEL0H01030)

PPA:	PAS_chr4_0595

YLI:	YALI0B15972g

SLB:	AWJ20_2030

TML:	GSTUM_00002772001

SRE:	PTSG_02129

DDI:	DDB_G0276919(pldG)

DPP:	DICPUDRAFT_96554

DFA:	DFA_04808(pldG)

ACAN:

ACA1_147710

SMIN:

v1.2.036833.t1(symbB.v1.2.036833.t1)

NOC:	Noc_2092 Noc_2093

» show all

Reference

1 [PMID:3422494]

Authors

Low MG, Prasad AR.

Title

A phospholipase D specific for the phosphatidylinositol anchor of cell-surface proteins is abundant in plasma.

Journal

Proc Natl Acad Sci U S A 85:980-4 (1988)
DOI:10.1073/pnas.85.4.980

Reference

2 [PMID:3028377]

Authors

Malik AS, Low MG.

Title

Conversion of human placental alkaline phosphatase from a high Mr form to a low Mr form during butanol extraction. An investigation of the role of endogenous phosphoinositide-specific phospholipases.

Journal

Biochem J 240:519-27 (1986)

Reference

3 [PMID:7961859]

Authors

Li JY, Hollfelder K, Huang KS, Low MG.

Title

Structural features of GPI-specific phospholipase D revealed by proteolytic fragmentation and Ca2+ binding studies.

Journal

J Biol Chem 269:28963-71 (1994)

Reference

4 [PMID:11254757]

Authors

Deeg MA, Bierman EL, Cheung MC.

Title

GPI-specific phospholipase D associates with an apoA-I- and apoA-IV-containing complex.

Journal

J Lipid Res 42:442-51 (2001)

Other DBs

ExplorEnz - The Enzyme Database:

3.1.4.50

IUBMB Enzyme Nomenclature:

3.1.4.50

ExPASy - ENZYME nomenclature database:

3.1.4.50

BRENDA, the Enzyme Database:

3.1.4.50

CAS:	113756-14-2

DBGET integrated database retrieval system