Entry |
|
Name |
glycosylphosphatidylinositol phospholipase D;
GPI-PLD;
glycoprotein phospholipase D;
phosphatidylinositol phospholipase D;
phosphatidylinositol-specific phospholipase D
|
Class |
Hydrolases;
Acting on ester bonds;
Phosphoric-diester hydrolases
|
Sysname |
glycoprotein-phosphatidylinositol phosphatidohydrolase
|
Reaction(IUBMB) |
6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + H2O = 6-(alpha-D-glucosaminyl)-1D-myo-inositol + 3-sn-phosphatidate [RN: R06623]
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Reaction(KEGG) |
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Substrate |
6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol [CPD: C04248];
H2O [CPD: C00001]
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Product |
6-(alpha-D-glucosaminyl)-1D-myo-inositol [CPD: C15658];
3-sn-phosphatidate [CPD: C00416]
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Comment |
This enzyme is also active when O-4 of the glucosamine is substituted by carrying the oligosaccharide that can link a protein to the structure. It therefore cleaves proteins from the lipid part of the glycosylphosphatidylinositol (GPI) anchors, but does so by hydrolysis, whereas glycosylphosphatidylinositol diacylglycerol-lyase (EC 4.6.1.14) does so by elimination. It acts on plasma membranes only after solubilization of the substrate with detergents or solvents, but it may act on intracellular membranes.
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History |
EC 3.1.4.50 created 1990, modified 2002
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Pathway |
ec00563 | Glycosylphosphatidylinositol (GPI)-anchor biosynthesis |
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Orthology |
K01127 | glycosylphosphatidylinositol phospholipase D |
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Genes |
» show all
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Reference |
|
Authors |
Low MG, Prasad AR |
Title |
A phospholipase D specific for the phosphatidylinositol anchor of cell-surface proteins is abundant in plasma. |
Journal |
|
Reference |
|
Authors |
Malik AS, Low MG. |
Title |
Conversion of human placental alkaline phosphatase from a high Mr form to a low Mr form during butanol extraction. An investigation of the role of endogenous phosphoinositide-specific phospholipases. |
Journal |
|
Reference |
|
Authors |
Li JY, Hollfelder K, Huang KS, Low MG. |
Title |
Structural features of GPI-specific phospholipase D revealed by proteolytic fragmentation and Ca2+ binding studies. |
Journal |
J Biol Chem 269:28963-71 (1994) |
Reference |
|
Authors |
Deeg MA, Bierman EL, Cheung MC. |
Title |
GPI-specific phospholipase D associates with an apoA-I- and apoA-IV-containing complex. |
Journal |
J Lipid Res 42:442-51 (2001) |
Other DBs |
ExplorEnz - The Enzyme Database: | 3.1.4.50 |
ExPASy - ENZYME nomenclature database: | 3.1.4.50 |
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