Hydrolases;
Acting on ester bonds;
Phosphoric-diester hydrolases
BRITE hierarchy

glycoprotein-phosphatidylinositol phosphatidohydrolase

6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + H2O = 6-(alpha-D-glucosaminyl)-1D-myo-inositol + 3-sn-phosphatidate [RN:R06623]

R06623(G);
(other) R04149 R07398
Reaction

6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol [CPD:C04248];
H2O [CPD:C00001]

This enzyme is also active when O-4 of the glucosamine is substituted by carrying the oligosaccharide that can link a protein to the structure. It therefore cleaves proteins from the lipid part of the glycosylphosphatidylinositol (GPI) anchors, but does so by hydrolysis, whereas glycosylphosphatidylinositol diacylglycerol-lyase (EC 4.6.1.14) does so by elimination. It acts on plasma membranes only after solubilization of the substrate with detergents or solvents, but it may act on intracellular membranes.

EC 3.1.4.50 created 1990, modified 2002

1  [PMID:3422494]

Low MG, Prasad AR

A phospholipase D specific for the phosphatidylinositol anchor of cell-surface proteins is abundant in plasma.

Proc Natl Acad Sci U S A 85:980-4 (1988)
DOI:10.1073/pnas.85.4.980

2  [PMID:3028377]

Malik AS, Low MG.

Conversion of human placental alkaline phosphatase from a high Mr form to a low Mr form during butanol extraction. An investigation of the role of endogenous phosphoinositide-specific phospholipases.

Biochem J 240:519-27 (1986)
DOI:10.1042/bj2400519

3  [PMID:7961859]

Li JY, Hollfelder K, Huang KS, Low MG.

Structural features of GPI-specific phospholipase D revealed by proteolytic fragmentation and Ca2+ binding studies.

J Biol Chem 269:28963-71 (1994)

4  [PMID:11254757]

Deeg MA, Bierman EL, Cheung MC.

GPI-specific phospholipase D associates with an apoA-I- and apoA-IV-containing complex.

J Lipid Res 42:442-51 (2001)