KEGG   ENZYME: 3.2.1.107Help
Entry
EC 3.2.1.107                Enzyme                                 

Name
protein-glucosylgalactosylhydroxylysine glucosidase;
PGGHG (gene name);
2-O-alpha-D-glucopyranosyl-5-O-alpha-D-galactopyranosylhydroxy-L-lysine glucohydrolase;
protein-alpha-D-glucosyl-1,2-beta-D-galactosyl-L-hydroxylysine glucohydrolase;
protein-alpha-D-glucosyl-(1->2)-beta-D-galactosyl-L-hydroxylysine glucohydrolase
Class
Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
BRITE hierarchy
Sysname
[collagen]-(5R)-5-O-[alpha-D-glucosyl-(1->2)-beta-D-galactosyl]-5-hydroxy-L-lysine glucohydrolase
Reaction(IUBMB)
[collagen]-(5R)-5-O-[alpha-D-glucosyl-(1->2)-beta-D-galactosyl]-5-hydroxy-L-lysine + H2O = D-glucose + [collagen]-(5R)-5-O-(beta-D-galactosyl)-5-hydroxy-L-lysine [RN:R04503]
Reaction(KEGG)
Substrate
[collagen]-(5R)-5-O-[alpha-D-glucosyl-(1->2)-beta-D-galactosyl]-5-hydroxy-L-lysine;
H2O [CPD:C00001]
Product
D-glucose [CPD:C00031];
[collagen]-(5R)-5-O-(beta-D-galactosyl)-5-hydroxy-L-lysine
Comment
The enzyme specifically hydrolyses glucose from alpha-D-glucosyl-(1->2)-beta-D-galactosyl disaccharide units that are linked to hydroxylysine residues of collagen and collagen-like proteins. Acetylation of the epsilon-amino group of the glycosylated hydroxylysine abolishes activity.
History
EC 3.2.1.107 created 1984
Orthology
K22078  protein-glucosylgalactosylhydroxylysine glucosidase
Genes
HSA: 80162(PGGHG)
PTR: 743704(PGGHG)
PPS: 100971508(PGGHG)
GGO: 109025438(PGGHG)
PON: 100454348(PGGHG)
NLE: 100601940(PGGHG)
MCC: 697199(PGGHG)
MCF: 101926478(PGGHG)
CSAB: 103236799(PGGHG)
RRO: 104655560(PGGHG)
RBB: 108537993(PGGHG)
CJC: 100385744(PGGHG)
SBQ: 101049147(ATHL1)
MMU: 212974(Pgghg)
RNO: 309103(Pgghg)
CGE: 100757546(Athl1)
NGI: 103751062(Pgghg)
HGL: 101723837(Pgghg)
CCAN: 109698993(Pgghg)
OCU: 103347496(PGGHG)
TUP: 102501509(PGGHG)
CFA: 483395(PGGHG)
AML: 100469466(PGGHG)
UMR: 103671029(PGGHG)
ORO: 101386980(ATHL1)
FCA: 101087100(PGGHG)
PTG: 102949755(PGGHG)
AJU: 106987954(PGGHG)
BTA: 511919(PGGHG)
BOM: 102276155(PGGHG)
PHD: 102324274(PGGHG)
CHX: 102188292(PGGHG)
OAS: 101106382(ATHL1)
SSC: 100518005(PGGHG)
CFR: 102511585(PGGHG)
CDK: 105105739(PGGHG)
BACU: 103010340(PGGHG)
LVE: 103071239(PGGHG)
OOR: 101279414(ATHL1)
ECB: 100146757(PGGHG)
EPZ: 103568056(PGGHG)
EAI: 106843884(PGGHG)
MYB: 102252209(PGGHG)
MYD: 102760826(PGGHG)
HAI: 109378078(PGGHG)
RSS: 109439220(PGGHG)
PALE: 102887768(PGGHG)
LAV: 100655235(PGGHG)
TMU: 101349759
MDO: 103092300(PGGHG)
SHR: 100923289(PGGHG)
OAA: 100091579(PGGHG)
GGA: 422991(PGGHG)
MGP: 100538987(PGGHG)
CJO: 107314276(PGGHG)
APLA: 101791880(PGGHG)
ACYG: 106047048(ATHL1)
TGU: 100219083(ATHL1) 100221918(ATHL1)
GFR: 102035352(PGGHG)
FAB: 101806950(PGGHG)
PHI: 102112573(PGGHG)
PMAJ: 107205256(PGGHG)
CCW: 104684542(PGGHG)
FPG: 101919210(PGGHG)
FCH: 102051782(PGGHG)
CLV: 102089207(PGGHG)
EGZ: 104127885(ATHL1)
AAM: 106489147(PGGHG)
ASN: 102382785(PGGHG)
AMJ: 102566701(PGGHG)
PSS: 102452333(PGGHG)
CMY: 102947357(PGGHG)
CPIC: 101949278(PGGHG)
ACS: 100552045(pgghg)
PVT: 110086184(PGGHG)
PBI: 103064802(PGGHG)
GJA: 107112736(PGGHG)
XLA: 108705477
XTR: 100496278(pgghg)
NPR: 108784117(PGGHG)
DRE: 777617(pgghg)
SRX: 107726850(athl1) 107754371
SANH: 107674819
SGH: 107551409
CCAR: 109071510(pgghg)
IPU: 108274703(pgghg)
AMEX: 103031131(pgghg)
TRU: 101062094(pgghg)
LCO: 104928845(pgghg)
NCC: 104956195(athl1)
MZE: 101468880(pgghg)
OLA: 101155845(pgghg)
XMA: 102229355(pgghg)
PRET: 103466501(pgghg)
NFU: 107388675(athl1)
CSEM: 103380281(athl1)
HCQ: 109507601(pgghg)
BPEC: 110169653(pgghg)
SASA: 106561823(pgghg)
ELS: 105029689(pgghg)
SFM: 108940848(pgghg)
LCM: 102363080(PGGHG)
CMK: 103174850(pgghg)
CIN: 108950085
SPU: 762741
APLC: 110983330
DME: Dmel_CG16965(CG16965)
DSI: Dsimw501_GD23801(Dsim_GD23801)
MDE: 101889101
BMOR: 101739191
PMAC: 106718814
PRAP: 110993009
FCD: 110848403
OBI: 106873889
HMG: 100207176
DDI: DDB_G0287109(athl1)
DFA: DFA_10677(athl1)
NGR: NAEGRDRAFT_69774(AM32)
MUC: MuYL_1472
MGOT: MgSA37_02000(kojP_1)
 » show all
Taxonomy
Reference
1  [PMID:385589]
  Authors
Hamazaki H, Hotta K.
  Title
Purification and characterization of an alpha-glucosidase specific for hydroxylysine-linked disaccharide of collagen.
  Journal
J Biol Chem 254:9682-7 (1979)
Reference
2  [PMID:7460918]
  Authors
Hamazaki H, Hotta K.
  Title
Enzymatic hydrolysis of disaccharide unit of collagen. Isolation of 2-O-alpha-D-glucopyranosyl-O-beta-D-galactopyranosyl-hydroxylysine glucohydrolase from rat spleens.
  Journal
Eur J Biochem 111:587-91 (1980)
DOI:10.1111/j.1432-1033.1980.tb04975.x
Reference
3  [PMID:385599]
  Authors
Sternberg M, Spiro RG.
  Title
Studies on the catabolism of the hydroxylysine-linked disaccharide units of basement membranes and collagens. Isolation and characterization of a rat kidney alpha-glucosidase of high specificity.
  Journal
J Biol Chem 254:10329-36 (1979)
Reference
4  [PMID:26682924]
  Authors
Hamazaki H, Hamazaki MH
  Title
Catalytic site of human protein-glucosylgalactosylhydroxylysine glucosidase: Three crucial carboxyl residues were determined by cloning and site-directed mutagenesis.
  Journal
Biochem Biophys Res Commun 469:357-62 (2016)
DOI:10.1016/j.bbrc.2015.12.005
  Sequence
[gga:422991]
Other DBs
ExplorEnz - The Enzyme Database: 3.2.1.107
IUBMB Enzyme Nomenclature: 3.2.1.107
ExPASy - ENZYME nomenclature database: 3.2.1.107
BRENDA, the Enzyme Database: 3.2.1.107
CAS: 72829-45-9

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