EC                Enzyme                                 

mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase;
MAN2A1 (gene name);
MAN2A2 (gene name);
mannosidase II;
alpha-D-mannosidase II;
alpha-mannosidase II;
GlcNAc transferase I-dependent alpha1,3[alpha1,6]mannosidase;
Golgi alpha-mannosidase II;
1,3-(1,6-)mannosyl-oligosaccharide alpha-D-mannohydrolase;
(1->3)-(1->6)-mannosyl-oligosaccharide alpha-D-mannohydrolase
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
BRITE hierarchy
(1->3)-(1->6)-mannosyl-oligosaccharide alpha-D-mannohydrolase (configuration-retaining)
Man5GlcNAc3-[protein] + 2 H2O = Man3GlcNAc3-[protein] + 2 alpha-D-mannopyranose [RN:R05984]
H2O [CPD:C00001]
alpha-D-mannopyranose [CPD:C00936]
The enzyme, found in plants and animals, participates in the processing of N-glycans in the Golgi apparatus. It removes two mannosyl residues, one linked by alpha1,3 linkage, and the other linked by alpha1,6 linkage, both of which are removed by the same catalytic site. The enzyme is sensitive to swainsonine.
EC created 1986, modified 2018
ec00510  N-Glycan biosynthesis
ec00513  Various types of N-glycan biosynthesis
ec01100  Metabolic pathways
K01231  alpha-mannosidase II
HSA: 4122(MAN2A2) 4124(MAN2A1)
PTR: 461982(MAN2A1) 745986(MAN2A2)
PPS: 100973826(MAN2A1) 100975142(MAN2A2)
GGO: 101130735(MAN2A2) 101130746(MAN2A1)
PON: 100449341(MAN2A2) 100455258(MAN2A1)
NLE: 100581120(MAN2A1) 100598002(MAN2A2) 105739922
MCC: 705480(MAN2A1) 715720(MAN2A2)
MCF: 101865831(MAN2A1) 102118107(MAN2A2)
CSAB: 103231182(MAN2A2) 103244316(MAN2A1)
RRO: 104675604(MAN2A1) 104679282(MAN2A2)
CJC: 100394604(MAN2A2) 100415670(MAN2A1)
SBQ: 101028436(MAN2A1) 101032034(MAN2A2)
MMU: 140481(Man2a2) 17158(Man2a1)
RNO: 25478(Man2a1) 308757(Man2a2)
CGE: 100757006(Man2a1) 100764014(Man2a2)
NGI: 103749886(Man2a2) 103752287(Man2a1)
HGL: 101697315(Man2a1) 101717094(Man2a2)
CCAN: 109695085(Man2a2) 109699084(Man2a1)
OCU: 100345634(MAN2A2) 100350334(MAN2A1)
TUP: 102469872(MAN2A2) 102485972(MAN2A1)
CFA: 488745(MAN2A2) 488878(MAN2A1)
AML: 100474640(MAN2A1) 100475150(MAN2A2)
UMR: 103660265(MAN2A2) 103675784(MAN2A1)
ORO: 101368244(MAN2A1) 101371875(MAN2A2)
FCA: 101086451(MAN2A1) 101101317(MAN2A2)
PTG: 102953845(MAN2A1) 102959757(MAN2A2) 102964243
AJU: 106965940(MAN2A2) 106975671(MAN2A1)
BTA: 521539(MAN2A1) 527449(MAN2A2)
BOM: 102269911(MAN2A2) 102286530(MAN2A1)
BIU: 109562380(MAN2A1) 109575464(MAN2A2)
PHD: 102316836(MAN2A1) 102330792(MAN2A2)
CHX: 102171012(MAN2A2) 102182591(MAN2A1)
OAS: 101106798(MAN2A2) 101116572(MAN2A1)
SSC: 100154397(MAN2A2) 100514631(MAN2A1)
CFR: 102512093(MAN2A2) 102513706(MAN2A1)
CDK: 105094696(MAN2A2) 105095392(MAN2A1)
BACU: 103020657(MAN2A1) 103021128(MAN2A2)
LVE: 103074675(MAN2A2) 103076739(MAN2A1)
OOR: 101272169(MAN2A1) 101272204(MAN2A2)
ECB: 100053432(MAN2A2) 100064575(MAN2A1)
EPZ: 103546211(MAN2A2) 103551677(MAN2A1)
EAI: 106832048(MAN2A2) 106832412(MAN2A1)
MYB: 102239755(MAN2A2) 102241544(MAN2A1)
MYD: 102760778(MAN2A1) 102764752(MAN2A2)
HAI: 109371540(MAN2A1) 109381472(MAN2A2)
RSS: 109437115(MAN2A2) 109445407(MAN2A1)
PALE: 102878318(MAN2A1) 102884371(MAN2A2)
LAV: 100669338(MAN2A1) 100671385(MAN2A2)
MDO: 100010442(MAN2A1) 100014504(MAN2A2)
SHR: 100913308(MAN2A1) 100922248(MAN2A2) 105749370
OAA: 100082559(MAN2A1) 100090754(MAN2A2)
GGA: 415585(MAN2A2) 415611(MAN2A1)
MGP: 100539000(MAN2A2)
CJO: 107306350(MAN2A1) 107318993(MAN2A2)
APLA: 101790343(MAN2A2) 101792614(MAN2A1)
ACYG: 106042345(MAN2A1) 106048641(MAN2A2)
TGU: 100226019(MAN2A2) 100227845(MAN2A1)
SCAN: 103816135(MAN2A2) 103824196(MAN2A1)
GFR: 102041186(MAN2A1) 102041635(MAN2A2)
FAB: 101806895(MAN2A2) 101819402(MAN2A1)
PHI: 102101154(MAN2A2) 102113458(MAN2A1)
PMAJ: 107209587(MAN2A2) 107216400(MAN2A1)
CCAE: 111940865(MAN2A1) 111943021(MAN2A2)
CCW: 104683853(MAN2A2) 104693206(MAN2A1)
FPG: 101913159(MAN2A2) 101915871(MAN2A1) 101921020 106112093
FCH: 102048168(MAN2A2) 102050767(MAN2A1)
CLV: 102090971(MAN2A2) 102097702(MAN2A1)
EGZ: 104133484(MAN2A2) 104135037(MAN2A1)
NNI: 104017021(MAN2A2) 104022201(MAN2A1)
ACUN: 113484037(MAN2A2) 113489918(MAN2A1)
ASN: 102369470(MAN2A1) 102383379(MAN2A2)
AMJ: 102561641(MAN2A1) 102566705(MAN2A2)
PSS: 102459905(MAN2A1)
CMY: 102941559(MAN2A2) 102944199(MAN2A1)
CPIC: 101937670(MAN2A1) 101953630(MAN2A2) 101953998
ACS: 100555933(man2a1) 100568077(man2a2)
PVT: 110072017(MAN2A1) 110084439(MAN2A2)
PBI: 103053403 103053695(MAN2A1) 103058391(MAN2A2)
PMUR: 107289784(MAN2A2) 107293675(MAN2A1) 107301179
GJA: 107120457(MAN2A1) 107125467(MAN2A2)
XLA: 108707218(man2a1.S) 108712863(man2a2.S) 108717353(man2a1.L) 443991(man2a2.L)
XTR: 100036612(man2a1) 448465(man2a2)
NPR: 108789334(MAN2A1) 108798147(MAN2A2)
DRE: 100333987(man2a2) 560219(man2a1)
SANH: 107663767 107670215 107673231(man2a2) 107691725(man2a1)
IPU: 108274717(man2a2)
PHYP: 113529699(man2a2)
AMEX: 103023301(man2a1) 103041860(man2a2)
EEE: 113572463 113588518(man2a2)
TRU: 101061792(man2a2) 101077627(man2a1)
LCO: 104925267(man2a2) 104932414
MZE: 101474154 101483319(man2a2)
OLA: 101158104(man2a2) 101174073
XMA: 102218498(man2a1) 102232197(man2a2)
PRET: 103460455(man2a1) 103466353(man2a2)
NFU: 107374983(man2a1) 107388979(man2a2)
KMR: 108228395 108239965(man2a2)
CSEM: 103380513(man2a2) 103390365(man2a1)
LCF: 108874626(man2a1) 108875305(man2a2)
SDU: 111228707(man2a2) 111238819(man2a1)
HCQ: 109530362(man2a2)
MALB: 109954216 109968742(man2a2) 109973112(man2a1)
SASA: 106561301 106562091(man2a1) 106568341 106573342(man2a2)
SALP: 111952337(man2a2) 111961325(man2a1) 111962448 111981710
ELS: 105018384(man2a2) 105027936(man2a1)
SFM: 108932015(man2a1) 108941123(man2a2)
PKI: 111835779(man2a1) 111845078(man2a2)
CMK: 103178412(man2a1) 103188552(man2a2)
CIN: 100180083
SPU: 589339
APLC: 110979321
SKO: 102802038
DME: Dmel_CG18802(alpha-Man-IIa)
DER: 6552301
DPE: 6595142
DSI: Dsimw501_GD18615(Dsim_GD18615)
DWI: 6647583
MDE: 101898268
AAG: 5575653
AME: 552382
BIM: 100749601
BTER: 100646824
SOC: 105198479
MPHA: 105836778
AEC: 105154407
ACEP: 105623777
PBAR: 105423365
HST: 105190974
DQU: 106750368
CFO: 105253605
LHU: 105674564
PGC: 109851679
OBO: 105274664
PCF: 106789840
NVI: 100116564
TCA: 660728
DPA: 109544976
NVL: 108562674
BMOR: 101742043
PMAC: 106721685
PRAP: 110993078
HAW: 110380432
TNL: 113499077
PXY: 105380392
API: 100169064
DNX: 107161638
CLEC: 106666206
ZNE: 110835247
FCD: 110848037
TUT: 107360206
DPTE: 113797886
CEL: CELE_F58H1.1(aman-2)
CBR: CBG23330(Cbr-aman-2)
BMY: Bm1_29570
TSP: Tsp_10745
SHX: MS3_01222
EGL: EGR_08871
EPA: 110234138
ADF: 107347367
PDAM: 113670726
SPIS: 111324658
ATH: AT5G14950(GMII)
CRB: 17881567
THJ: 104821170
CPAP: 110823589
CIT: 102615196
TCC: 18601342
EGR: 104422581
VRA: 106770832
VAR: 108345656
CCAJ: 109817438
CAM: 101499632(MAN2A2)
LJA: Lj2g3v1022240.1(Lj2g3v1022240.1)
ADU: 107471073
AIP: 107622688
FVE: 101296768
PPER: 18782416
PMUM: 103329931
PAVI: 110756683
PXB: 103928463
ZJU: 107415425
CSV: 101208332(MAN2A2)
CMO: 103487047
MCHA: 111018346
RCU: 8280600
JCU: 105642251
MESC: 110601253
JRE: 108992841
VVI: 100243903(MAN2A2)
SLY: 101266287
SPEN: 107011510
SOT: 102605224
CANN: 107860595
NSY: 104211404
NTO: 104108022
SIND: 105177181
OEU: 111409226
HAN: 110903921
LSV: 111891915
CCAV: 112523600
DCR: 108214457
BVG: 104905356
SOE: 110786831
NNU: 104607979
OSA: 4340636
DOSA: Os06t0245700-00(Os06g0245700)
OBR: 102707404
BDI: 100822520
ATS: 109783252(LOC109783252)
SBI: 8061493
SITA: 101761357
PDA: 103710003
MUS: 103990861
DCT: 110092430
AOF: 109820905
ATR: 18446724
PPP: 112275693
DFA: DFA_02000 DFA_07693(manD)
 » show all
1  [PMID:863880]
Tulsiani DR, Opheim DJ, Touster O.
Purification and characterization of alpha-D-mannosidase from rat liver golgi membranes.
J Biol Chem 252:3227-33 (1977)
2  [PMID:212436]
Tabas I, Kornfeld S
The synthesis of complex-type oligosaccharides. III. Identification of an alpha-D-mannosidase activity involved in a late stage of processing of complex-type oligosaccharides.
J Biol Chem 253:7779-86 (1978)
3  [PMID:6445359]
Harpaz N, Schachter H.
Control of glycoprotein synthesis. Processing of asparagine-linked oligosaccharides by one or more rat liver Golgi alpha-D-mannosidases dependent on the prior action of UDP-N-acetylglucosamine: alpha-D-mannoside beta 2-N-acetylglucosaminyltransferase I.
J Biol Chem 255:4894-902 (1980)
4  [PMID:7061502]
Tulsiani DR, Hubbard SC, Robbins PW, Touster O.
alpha-D-Mannosidases of rat liver Golgi membranes. Mannosidase II is the GlcNAcMAN5-cleaving enzyme in glycoprotein biosynthesis and mannosidases Ia and IB are the enzymes converting Man9 precursors to Man5 intermediates.
J Biol Chem 257:3660-8 (1982)
5  [PMID:1757461]
Moremen KW, Robbins PW
Isolation, characterization, and expression of cDNAs encoding murine alpha-mannosidase II, a Golgi enzyme that controls conversion of high mannose to  complex N-glycans.
J Cell Biol 115:1521-34 (1991)
[hsa:4124] [mmu:17158]
6  [PMID:8524845]
Misago M, Liao YF, Kudo S, Eto S, Mattei MG, Moremen KW, Fukuda MN
Molecular cloning and expression of cDNAs encoding human alpha-mannosidase II and a previously unrecognized alpha-mannosidase IIx isozyme.
Proc Natl Acad Sci U S A 92:11766-70 (1995)
[hsa:4124 4122]
7  [PMID:11406577]
van den Elsen JM, Kuntz DA, Rose DR
Structure of Golgi alpha-mannosidase II: a target for inhibition of growth and metastasis of cancer cells.
EMBO J 20:3008-17 (2001)
8  [PMID:15721331]
Athanasopoulos VI, Niranjan K, Rastall RA.
The production, purification and characterisation of two novel alpha-D-mannosidases from Aspergillus phoenicis.
Carbohydr Res 340:609-17 (2005)
9  [PMID:18599462]
Shah N, Kuntz DA, Rose DR
Golgi alpha-mannosidase II cleaves two sugars sequentially in the same catalytic  site.
Proc Natl Acad Sci U S A 105:9570-5 (2008)
10 [PMID:22819743]
Rose DR
Structure, mechanism and inhibition of Golgi alpha-mannosidase II.
Curr Opin Struct Biol 22:558-62 (2012)
Other DBs
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IUBMB Enzyme Nomenclature:
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BRENDA, the Enzyme Database:
CAS: 82047-77-6

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