KEGG   ENZYME: 3.2.1.130Help
Entry
EC 3.2.1.130                Enzyme                                 

Name
glycoprotein endo-alpha-1,2-mannosidase;
glucosylmannosidase;
endo-alpha-D-mannosidase;
endo-alpha-mannosidase;
endomannosidase;
glucosyl mannosidase;
MANEA (gene name);
glycoprotein glucosylmannohydrolase
Class
Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
BRITE hierarchy
Sysname
glycoprotein glucosylmannohydrolase (configuration-retaining)
Reaction(IUBMB)
GlcMan9GlcNAc2-[protein] + H2O = Man8GlcNAc2-[protein] (isomer 8A1,2,3B1,2) + alpha-D-glucosyl-(1->3)-alpha-D-mannopyranose
Substrate
GlcMan9GlcNAc2-[protein];
H2O [CPD:C00001]
Product
Man8GlcNAc2-[protein] (isomer 8A1,2,3B1,2);
alpha-D-glucosyl-(1->3)-alpha-D-mannopyranose
Comment
The enzyme catalyses the hydrolysis of the terminal alpha-D-glucosyl-(1->3)-D-mannosyl unit from the GlcMan9(GlcNAc)2 oligosaccharide component of N-glucosylated proteins during their processing in the Golgi apparatus. The name for the isomer is based on a nomenclature proposed by Prien et al [7].
History
EC 3.2.1.130 created 1990, modified 2017
Orthology
K15538  glycoprotein endo-alpha-1,2-mannosidase
K21132  alpha-mannan endo-1,2-alpha-mannanase / glycoprotein endo-alpha-1,2-mannosidase
Genes
HSA: 79694(MANEA)
PTR: 472074(MANEA)
PPS: 100996027(MANEA)
GGO: 101153501(MANEA)
PON: 100174316(MANEA)
NLE: 100598520(MANEA)
MCC: 708164(MANEA)
MCF: 102131103(MANEA)
CSAB: 103240128(MANEA)
RRO: 104659172 104659438 104668264(MANEA)
RBB: 108538601(MANEA)
CJC: 100414257(MANEA)
SBQ: 101043274(MANEA)
MMU: 242362(Manea)
RNO: 140808(Manea)
CGE: 100682532(Manea)
NGI: 103739270(Manea)
HGL: 101724745(Manea)
CCAN: 109699781(Manea)
OCU: 100354038(MANEA)
TUP: 102470618(MANEA)
CFA: 481925(MANEA)
AML: 100465796(MANEA)
UMR: 103675891(MANEA)
ORO: 101368286(MANEA)
FCA: 101101567(MANEA)
PTG: 102953225(MANEA)
AJU: 106980082(MANEA)
BTA: 512853(MANEA)
BOM: 102266362(MANEA)
BIU: 109563524(MANEA)
PHD: 102325762(MANEA)
CHX: 102190879(MANEA)
OAS: 101106778(MANEA)
SSC: 100154996(MANEA)
CFR: 102505037(MANEA)
CDK: 105092263(MANEA)
BACU: 102997965
LVE: 103075786(MANEA)
OOR: 101282781(MANEA)
ECB: 100065922(MANEA)
EPZ: 103548352(MANEA)
EAI: 106832462(MANEA)
MYB: 102244343(MANEA)
MYD: 102761757(MANEA)
HAI: 109373533(MANEA)
RSS: 109454890(MANEA)
PALE: 102881260(MANEA)
LAV: 100666598(MANEA)
TMU: 101343162
MDO: 100026589(MANEA)
SHR: 100925449(MANEA)
OAA: 100073892(MANEA)
GGA: 421807(MANEA)
MGP: 100538669(MANEA)
CJO: 107311928(MANEA)
APLA: 101799379(MANEA)
ACYG: 106035412(MANEA)
TGU: 100227255(MANEA)
GFR: 102034556(MANEA)
FAB: 101815462(MANEA)
PHI: 102108129(MANEA)
PMAJ: 107201796(MANEA)
CCAE: 111925715(MANEA)
CCW: 104692141(MANEA)
FPG: 101917050(MANEA)
FCH: 102050839(MANEA)
CLV: 102091008(MANEA)
EGZ: 104135406(MANEA)
AAM: 106489716(MANEA)
ASN: 102374994(MANEA)
AMJ: 102571324(MANEA)
PSS: 102444522(MANEA)
CMY: 102943683(MANEA)
CPIC: 101940123(MANEA)
ACS: 100561214(manea)
PVT: 110074132(MANEA)
PBI: 103065662(MANEA)
GJA: 107121220(MANEA)
XLA: 446517(maneal.L)
XTR: 100485932(manea)
NPR: 108788904(MANEA)
DRE: 447827(manea)
IPU: 108270006(MANEA)
AMEX: 103022491
TRU: 101078369
LCO: 104931611
NCC: 104964384(manea)
MZE: 101476482
OLA: 101164688
XMA: 102223704(manea)
PRET: 103458747
NFU: 107392157
KMR: 108233348
CSEM: 103378701(manea)
LCF: 108874963(manea)
SDU: 111225671
BPEC: 110153642
MALB: 109968006
SASA: 106561892
OTW: 112263438(manea)
ELS: 105031619
SFM: 108933186
LCM: 102347276(MANEA)
CMK: 103178280(manea)
SPU: 591903
APLC: 110986171
SKO: 100377430
NVI: 100116153
MDL: 103576516
NVL: 108558901
CLEC: 106667568
ZNE: 110836294
FCD: 110855257
BMY: Bm1_20030
TSP: Tsp_10277
MYI: 110467305
OBI: 106874611
LAK: 106155033
SHX: MS3_00913
EPA: 110235549
ADF: 107347975
HMG: 100199440
SMIN: v1.2.001961.t1(symbB.v1.2.001961.t1)
NGR: NAEGRDRAFT_62591(FM155)
LAB: LA76x_2943(manEA)
SMAL: SMALA_0936
BTH: BT_3862
BXY: BXY_34140
CHZ: CHSO_4479
 » show all
Taxonomy
Reference
1  [PMID:3818665]
  Authors
Lubas WA, Spiro RG.
  Title
Golgi endo-alpha-D-mannosidase from rat liver, a novel N-linked carbohydrate unit processing enzyme.
  Journal
J Biol Chem 262:3775-81 (1987)
Reference
2  [PMID:2407194]
  Authors
Tulsiani DR, Coleman VD, Touster O.
  Title
Asparagine-linked glycoprotein biosynthesis in rat brain: identification of glucosidase I, glucosidase II, and and endomannosidase (glucosyl mannosidase).
  Journal
Arch Biochem Biophys 277:114-21 (1990)
DOI:10.1016/0003-9861(90)90558-G
Reference
3  [PMID:8106437]
  Authors
Hiraizumi S, Spohr U, Spiro RG
  Title
Ligand affinity chromatographic purification of rat liver Golgi endomannosidase.
  Journal
J Biol Chem 269:4697-700 (1994)
Reference
4  [PMID:9361017]
  Authors
Spiro MJ, Bhoyroo VD, Spiro RG
  Title
Molecular cloning and expression of rat liver endo-alpha-mannosidase, an N-linked oligosaccharide processing enzyme.
  Journal
J Biol Chem 272:29356-63 (1997)
DOI:10.1074/jbc.272.46.29356
  Sequence
[rno:140808]
Reference
5  [PMID:15677381]
  Authors
Hamilton SR, Li H, Wischnewski H, Prasad A, Kerley-Hamilton JS, Mitchell T, Walling AJ, Davidson RC, Wildt S, Gerngross TU
  Title
Intact {alpha}-1,2-endomannosidase is a typical type II membrane protein.
  Journal
Glycobiology 15:615-24 (2005)
DOI:10.1093/glycob/cwi045
  Sequence
[hsa:79694] [rno:140808]
Reference
6  [PMID:15760709]
  Authors
Hardt B, Volker C, Mundt S, Salska-Navarro M, Hauptmann M, Bause E
  Title
Human endo-alpha1,2-mannosidase is a Golgi-resident type II membrane protein.
  Journal
Biochimie 87:169-79 (2005)
DOI:10.1016/j.biochi.2004.11.004
  Sequence
[hsa:79694]
Reference
7  [PMID:19181540]
  Authors
Prien JM, Ashline DJ, Lapadula AJ, Zhang H, Reinhold VN
  Title
The high mannose glycans from bovine ribonuclease B isomer characterization by ion trap MS.
  Journal
J Am Soc Mass Spectrom 20:539-56 (2009)
DOI:10.1016/j.jasms.2008.11.012
Other DBs
ExplorEnz - The Enzyme Database: 3.2.1.130
IUBMB Enzyme Nomenclature: 3.2.1.130
ExPASy - ENZYME nomenclature database: 3.2.1.130
BRENDA, the Enzyme Database: 3.2.1.130
CAS: 108022-16-8

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