KEGG   ENZYME: 3.2.1.152Help
Entry
EC 3.2.1.152                Enzyme                                 

Name
mannosylglycoprotein endo-beta-mannosidase;
endo-beta-mannosidase
Class
Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
BRITE hierarchy
Reaction(IUBMB)
Hydrolysis of the alpha-D-mannosyl-(1->6)-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl sequence of glycoprotein to alpha-D-mannosyl-(1->6)-D-mannose and N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl sequences
Comment
The substrate group is a substituent on N-4 of an asparagine residue in the glycoprotein. The mannose residue at the non-reducing end of the sequence may carry further alpha-D-mannosyl groups on O-3 or O-6, but such a substituent on O-3 of the beta-D-mannosyl group prevents the action of the enzyme. The enzyme was obtained from the lily, Lilium longiflorum.
History
EC 3.2.1.152 created 2005
Orthology
K18577  mannosylglycoprotein endo-beta-mannosidase
Genes
ATH: AT1G09010
ALY: ARALYDRAFT_888134
CRB: 17900667
CSAT: 104739491 104755088 104767262 109131935
EUS: EUTSA_v10006730mg
BRP: 103836364 103871635
BNA: 106346294 106362335 106415431 106453177
BOE: 106294903 106307139
THJ: 104805875
CPAP: 110815331
CIT: 102618119
TCC: 18600378
GRA: 105761023 105781536(ManA2)
LJA: Lj1g3v3105090.1(Lj1g3v3105090.1) Lj1g3v4931610.1(Lj1g3v4931610.1) Lj5g3v1494660.1(Lj5g3v1494660.1)
FVE: 101296018
PAVI: 110759657
PXB: 103942899
CSV: 101207955
CMO: 103492174
MCHA: 111015278
CMAX: 111468316
CMOS: 111433409
CPEP: 111792852
RCU: 8285565
JCU: 105647543
HBR: 110644505
POP: 7481756
JRE: 109008073
VVI: 100254646
SLY: 101267848
SPEN: 107008011
SOT: 102586571
CANN: 107854888
NSY: 104218593
INI: 109181437
HAN: 110920122
LSV: 111895788
CCAV: 112507203
BVG: 104890840
SOE: 110798590
NNU: 104596494
OSA: 4338683
DOSA: Os05t0391500-01(Os05g0391500)
OBR: 102703868
BDI: 100834607
ATS: 109774075(LOC109774075) 109776035(LOC109776035)
SBI: 8085086
ZMA: 103630319
SITA: 101785350
PDA: 103716435
EGU: 105057570
MUS: 103977642
AOF: 109840056
ATR: 18440882
 » show all
Taxonomy
Reference
1  [PMID:15247239]
  Authors
Ishimizu T, Sasaki A, Okutani S, Maeda M, Yamagishi M, Hase S.
  Title
Endo-beta-mannosidase, a plant enzyme acting on N-glycan: purification, molecular cloning, and characterization.
  Journal
J Biol Chem 279:38555-62 (2004)
DOI:10.1074/jbc.M406886200
  Sequence
[ath:AT1G09010]
Reference
2  [PMID:9990135]
  Authors
Sasaki A, Yamagishi M, Mega T, Norioka S, Natsuka S, Hase S.
  Title
Partial purification and characterization of a novel endo-beta-mannosidase acting on N-linked sugar chains from Lilium longflorum thumb.
  Journal
J Biochem (Tokyo) 125:363-7 (1999)
Other DBs
ExplorEnz - The Enzyme Database: 3.2.1.152
IUBMB Enzyme Nomenclature: 3.2.1.152
ExPASy - ENZYME nomenclature database: 3.2.1.152
BRENDA, the Enzyme Database: 3.2.1.152
CAS: 141176-95-6

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