KEGG   ENZYME: 3.2.1.171Help
Entry
EC 3.2.1.171                Enzyme                                 

Name
rhamnogalacturonan hydrolase;
rhamnogalacturonase A;
RGase A;
RG-hydrolase
Class
Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
BRITE hierarchy
Sysname
rhamnogalacturonan alpha-D-GalA-(1->2)-alpha-L-Rha hydrolase
Reaction(IUBMB)
Endohydrolysis of alpha-D-GalA-(1->2)-alpha-L-Rha glycosidic bond in the rhamnogalacturonan I backbone with initial inversion of anomeric configuration releasing oligosaccharides with beta-D-GalA at the reducing end.
Comment
The enzyme is part of the degradation system for rhamnogalacturonan I in Aspergillus aculeatus.
History
EC 3.2.1.171 created 2011
Orthology
K18580  rhamnogalacturonan hydrolase
Genes
TTT: THITE_156575 THITE_76240
TMN: UCRPA7_3596
SSCK: SPSK_05216
FGR: FGSG_04689
FVR: FVEG_03362
FOX: FOXG_04765
NHE: NECHADRAFT_86762
CFJ: CFIO01_07103
PFY: PFICI_03152 PFICI_10114 PFICI_11675
SSL: SS1G_02399 SS1G_07039 SS1G_08229
ANI: AN9134.2
ANG: ANI_1_1364104(An12g00950) ANI_1_604124(An14g04200)
PTE: PTT_07842
ZTR: MYCGRDRAFT_77196(MgRHA1)
 » show all
Taxonomy
Reference
1  [PMID:9115442]
  Authors
Petersen TN, Kauppinen S, Larsen S
  Title
The crystal structure of rhamnogalacturonase A from Aspergillus aculeatus: a right-handed parallel beta helix.
  Journal
Structure 5:533-44 (1997)
DOI:10.1016/S0969-2126(97)00209-8
  Sequence
Reference
2  [PMID:7961884]
  Authors
Kofod LV, Kauppinen S, Christgau S, Andersen LN, Heldt-Hansen HP, Dorreich K, Dalboge H
  Title
Cloning and characterization of two structurally and functionally divergent rhamnogalacturonases from Aspergillus aculeatus.
  Journal
J Biol Chem 269:29182-9 (1994)
  Sequence
Reference
3  [PMID:8720076]
  Authors
Azadi P, O'Neill MA, Bergmann C, Darvill AG, Albersheim P
  Title
The backbone of the pectic polysaccharide rhamnogalacturonan I is cleaved by an endohydrolase and an endolyase.
  Journal
Glycobiology 5:783-9 (1995)
DOI:10.1093/glycob/5.8.783
  Sequence
Reference
4  [PMID:15299976]
  Authors
Petersen TN, Christgau S, Kofod LV, Kauppinen S, Johnson AH, Larsen S
  Title
Crystallization and preliminary X-ray studies of rhamnogalacturonase A from Aspergillus aculeatus.
  Journal
Acta Crystallogr D Biol Crystallogr 53:105-7 (1997)
DOI:10.1107/S0907444996010785
Reference
5  [PMID:9464254]
  Authors
Pitson SM, Mutter M, van den Broek LA, Voragen AG, Beldman G
  Title
Stereochemical course of hydrolysis catalysed by alpha-L-rhamnosyl and alpha-D-galacturonosyl hydrolases from Aspergillus aculeatus.
  Journal
Biochem Biophys Res Commun 242:552-9 (1998)
DOI:10.1006/bbrc.1997.8009
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 3.2.1.171
IUBMB Enzyme Nomenclature: 3.2.1.171
ExPASy - ENZYME nomenclature database: 3.2.1.171
BRENDA, the Enzyme Database: 3.2.1.171

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