KEGG   ENZYME: 3.2.1.176Help
Entry
EC 3.2.1.176                Enzyme                                 

Name
cellulose 1,4-beta-cellobiosidase (reducing end);
CelS;
CelSS;
endoglucanase SS;
cellulase SS;
cellobiohydrolase CelS;
Cel48A
Class
Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
BRITE hierarchy
Sysname
4-beta-D-glucan cellobiohydrolase (reducing end)
Reaction(IUBMB)
Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and similar substrates, releasing cellobiose from the reducing ends of the chains.
Comment
Some exocellulases, most of which belong to the glycoside hydrolase family 48 (GH48, formerly known as cellulase family L), act at the reducing ends of cellulose and similar substrates. The CelS enzyme from Clostridium thermocellum is the most abundant subunit of the cellulosome formed by the organism. It liberates cellobiose units from the reducing end by hydrolysis of the glycosidic bond, employing an inverting reaction mechanism [2]. Different from EC 3.2.1.91, which attacks cellulose from the non-reducing end.
History
EC 3.2.1.176 created 2011
Orthology
K20829  cellulose 1,4-beta-cellobiosidase
Genes
CTH: Cthe_2089
CTX: Clo1313_2747
Taxonomy
Reference
1  [PMID:8555231]
  Authors
Barr BK, Hsieh YL, Ganem B, Wilson DB
  Title
Identification of two functionally different classes of exocellulases.
  Journal
Biochemistry 35:586-92 (1996)
DOI:10.1021/bi9520388
Reference
2  [PMID:20967294]
  Authors
Saharay M, Guo H, Smith JC
  Title
Catalytic mechanism of cellulose degradation by a cellobiohydrolase, CelS.
  Journal
PLoS One 5:e12947 (2010)
DOI:10.1371/journal.pone.0012947
  Sequence
[cth:Cthe_2089]
Other DBs
ExplorEnz - The Enzyme Database: 3.2.1.176
IUBMB Enzyme Nomenclature: 3.2.1.176
ExPASy - ENZYME nomenclature database: 3.2.1.176
BRENDA, the Enzyme Database: 3.2.1.176

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