KEGG   ENZYME: 3.2.1.82Help
Entry
EC 3.2.1.82                 Enzyme                                 

Name
exo-poly-alpha-digalacturonosidase;
pehX (gene name);
poly(1,4-alpha-D-galactosiduronate) digalacturonohydrolase;
exopolygalacturonosidase (misleading);
poly[(1->4)-alpha-D-galactosiduronate] digalacturonohydrolase;
exo-poly-alpha-galacturonosidase
Class
Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
BRITE hierarchy
Sysname
poly[(1->4)-alpha-D-galactosiduronate] non-reducing-end-digalacturonohydrolase
Reaction(IUBMB)
[(1->4)-alpha-D-galacturonosyl]n + H2O = alpha-D-galacturonosyl-(1->4)-D-galacturonate + [(1->4)-alpha-D-galacturonosyl]n-2
Substrate
[(1->4)-alpha-D-galacturonosyl]n [CPD:C00470];
H2O [CPD:C00001]
Product
alpha-D-galacturonosyl-(1->4)-D-galacturonate;
[(1->4)-alpha-D-galacturonosyl]n-2
Comment
The enzyme, characterized from bacteria, hydrolyses the second alpha-1,4-glycosidic bond from the non-reducing end of polygalacturonate, releasing digalacturonate.
History
EC 3.2.1.82 created 1972, modified 2019
Orthology
K18650  exo-poly-alpha-galacturonosidase
Genes
KOX: KOX_13235
KOE: A225_1464
KOY: J415_24315
KOM: HR38_11985
KOK: KONIH1_07185
KOC: AB185_28860
KLL: BJF97_07630
YEN: YE0164(pehX)
YEP: YE105_C0167
YEY: Y11_33621
YEW: CH47_3321(pehX)
YET: CH48_1856(pehX)
YEE: YE5303_41081(pehX)
YIN: CH53_1967(pehX)
YRO: CH64_2753(pehX)
ECA: ECA3111(pehX)
PATR: EV46_15395
PATO: GZ59_31190(pehX)
PCT: PC1_2862
PEC: W5S_1293
DDD: Dda3937_00261(pehX) Dda3937_00262(pehW) Dda3937_00263(pehV)
PPF: Pput_1884
PPI: YSA_08947
PPX: T1E_2073(pehX)
PPUT: L483_21750
PFS: PFLU_2286(pehX)
PSES: PSCI_0280
RSO: RSc1756(pehB)
RSC: RCFBP_11765(pehB)
RSL: RPSI07_0327(pehB)
RSN: RSPO_c01791(pehB)
RSM: CMR15_11765(pehB)
RSE: F504_1633
CAC: CA_C3684
CAE: SMB_G3725(pehX)
CAY: CEA_G3691
CSB: CLSA_c18950(pehX)
TSH: Tsac_0344
PUF: UFO1_0808
PFT: JBW_03716
 » show all
Taxonomy
Reference
1  [PMID:5661621]
  Authors
Hasegawa S, Nagel CW.
  Title
Isolation of an oligogalacturonate hydrolase from a Bacillus specie.
  Journal
Arch Biochem Biophys 124:513-20 (1968)
DOI:10.1016/0003-9861(68)90360-3
Reference
2
  Authors
Hatanaka, C. and Ozawa, J.
  Title
Enzymic degradation of pectic acid. XIII. New exopolygalacturonase producing digalacturonic acid from pectic acid.
  Journal
J Agric Chem Soc Jpn 43:764-772 (1968)
Reference
3
  Authors
Hatanaka, C. and Ozawa, J.
  Title
Ber des O'Hara Inst 15:47 (1971)
Reference
4  [PMID:2168372]
  Authors
He SY, Collmer A
  Title
Molecular cloning, nucleotide sequence, and marker exchange mutagenesis of the exo-poly-alpha-D-galacturonosidase-encoding pehX gene of Erwinia chrysanthemi EC16.
  Journal
J Bacteriol 172:4988-95 (1990)
DOI:10.1128/JB.172.9.4988-4995.1990
Other DBs
ExplorEnz - The Enzyme Database: 3.2.1.82
IUBMB Enzyme Nomenclature: 3.2.1.82
ExPASy - ENZYME nomenclature database: 3.2.1.82
BRENDA, the Enzyme Database: 3.2.1.82
CAS: 37288-58-7

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