KEGG   ENZYME: 3.2.2.24Help
Entry
EC 3.2.2.24                 Enzyme                                 

Name
ADP-ribosyl-[dinitrogen reductase] hydrolase;
azoferredoxin glycosidase;
azoferredoxin-activating enzymes;
dinitrogenase reductase-activating glycohydrolase;
ADP-ribosyl glycohydrolase;
draG (gene name)
Class
Hydrolases;
Glycosylases;
Hydrolysing N-glycosyl compounds
BRITE hierarchy
Sysname
ADP-D-ribosyl-[dinitrogen reductase] ADP-ribosylhydrolase
Reaction(IUBMB)
[dinitrogen reductase]-Nomega-alpha-(ADP-D-ribosyl)-L-arginine = ADP-D-ribose + [dinitrogen reductase]-L-arginine [RN:R03446]
Reaction(KEGG)
Substrate
[dinitrogen reductase]-Nomega-alpha-(ADP-D-ribosyl)-L-arginine [CPD:C01253]
Product
ADP-D-ribose [CPD:C00301];
[dinitrogen reductase]-L-arginine [CPD:C03315]
Comment
The enzyme restores the activity of EC 1.18.6.1, nitrogenase, by catalysing the removal of ADP-ribose from an arginine residue of the dinitrogenase reductase component of nitrogenase. This activity occurs only when the nitrogenase product, ammonium, is not available. The combined activity of this enzyme and EC 2.4.2.37, NAD+-dinitrogen-reductase ADP-D-ribosyltransferase, controls the level of activity of nitrogenase.
History
EC 3.2.2.24 created 1992
Orthology
K05521  ADP-ribosyl-[dinitrogen reductase] hydrolase
Genes
SPE: Spro_3018
BTO: WQG_5510
BTRE: F542_16530
BTRH: F543_18240
BTRA: F544_5830
XCC: XCC2928(draG)
XCB: XC_1180
XCA: xcc-b100_1226
SMZ: SMD_1618
PPF: Pput_1073
PPT: PPS_2834
PPI: YSA_07149
PPX: T1E_2461
PPUH: B479_05450
PPUN: PP4_24590
PMON: X969_03535
PMOT: X970_03510
PFL: PFL_4115
PPRC: PFLCHA0_c41750(draG)
SSE: Ssed_2268
TAU: Tola_0577
PNA: Pnap_4684
RGE: RGE_42850(draG)
SLT: Slit_0832
DSU: Dsui_0953
DAR: Daro_1447
AZO: azo0530(draG1)
AZA: AZKH_0794(draG)
GSU: GSU2800(draG)
GSK: KN400_2737(draG)
GME: Gmet_0680(draG)
GUR: Gura_1208
GLO: Glov_0420
GBM: Gbem_2082(draG)
GEO: Geob_2589(draG)
GEM: GM21_2136
GEB: GM18_1870
PCA: Pcar_2093(draG)
PPD: Ppro_3462
DAT: HRM2_09820(draG1)
RPA: RPA2406(draG)
RPB: RPB_3043
RPC: RPC_0877
RPD: RPD_2408
RPE: RPE_0836
RPT: Rpal_2658
MEX: Mext_4887
MDI: METDI1913
RCP: RCAP_rcc03017(draG)
NAR: Saro_0259
RRU: Rru_A1008
RRF: F11_05195
MAG: amb1576
MGY: MGMSRv2__0347(draG)
AZL: AZL_007740(draG)
ALI: AZOLI_0685(draG)
ABS: AZOBR_70133(draG)
MGM: Mmc1_1210
AFR: AFE_1525(draG)
PPY: PPE_03341
PPO: PPM_3580(draG)
PMW: B2K_33220
PSAB: PSAB_03525
RUM: CK1_14850
STH: STH1516
DAE: Dtox_3565
ADG: Adeg_1446
TTM: Tthe_0504
TSH: Tsac_2819
FMA: FMG_0339
MMAR: MODMU_4120
AMR: AM1_1924
ATM: ANT_00250(draG)
TTR: Tter_0338
CAA: Caka_2844
MPL: Mpal_2490
NPH: NP_3878A
HUT: Huta_1731
HTI: HTIA_1743
HLA: Hlac_2793
SALI: L593_06395
 » show all
Taxonomy
Reference
1  [PMID:2506427]
  Authors
Fitzmaurice WP, Saari LL, Lowery RG, Ludden PW, Roberts GP.
  Title
Genes coding for the reversible ADP-ribosylation system of dinitrogenase reductase from Rhodospirillum rubrum.
  Journal
Mol Gen Genet 218:340-7 (1989)
  Sequence
[rru:Rru_A1008]
Reference
2  [PMID:19477184]
  Authors
Li XD, Huergo LF, Gasperina A, Pedrosa FO, Merrick M, Winkler FK
  Title
Crystal structure of dinitrogenase reductase-activating glycohydrolase (DraG) reveals conservation in the ADP-ribosylhydrolase fold and specific features in the ADP-ribose-binding pocket.
  Journal
J Mol Biol 390:737-46 (2009)
DOI:10.1016/j.jmb.2009.05.031
Reference
3  [PMID:19706507]
  Authors
Berthold CL, Wang H, Nordlund S, Hogbom M
  Title
Mechanism of ADP-ribosylation removal revealed by the structure and ligand complexes of the dimanganese mono-ADP-ribosylhydrolase DraG.
  Journal
Proc Natl Acad Sci U S A 106:14247-52 (2009)
DOI:10.1073/pnas.0905906106
Other DBs
ExplorEnz - The Enzyme Database: 3.2.2.24
IUBMB Enzyme Nomenclature: 3.2.2.24
ExPASy - ENZYME nomenclature database: 3.2.2.24
BRENDA, the Enzyme Database: 3.2.2.24
CAS: 125626-63-3

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