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Entry
EC 3.2.2.28                 Enzyme                                 

Name
double-stranded uracil-DNA glycosylase;
Mug;
double-strand uracil-DNA glycosylase;
Dug;
dsUDG;
double-stranded DNA specific UDG;
dsDNA specific UDG;
UdgB (ambiguous);
G:T/U mismatch-specific DNA glycosylase;
UDG (ambiguous)
Class
Hydrolases;
Glycosylases;
Hydrolysing N-glycosyl compounds
BRITE hierarchy
Sysname
uracil-double-stranded DNA deoxyribohydrolase (uracil-releasing)
Reaction(IUBMB)
Specifically hydrolyses mismatched double-stranded DNA and polynucleotides, releasing free uracil
Comment
No activity on DNA containing a T/G mispair or single-stranded DNA containing either a site-specific uracil or 3,N4-ethenocytosine residue [2], significant role for double-stranded uracil-DNA glycosylase in mutation avoidance in non-dividing E. coli [3]. Uracil-DNA glycosylases are widespread enzymes that are found in all living organisms. Uracil-DNA glycosylase (EC 3.2.2.27) and EC 3.2.2.28 form a central part of the DNA-repair machinery since they initiate the DNA base-excision repair pathway by hydrolysing the N-glycosidic bond between uracil and the deoxyribose sugar thereby catalysing the removal of mis-incorporated uracil from DNA.
History
EC 3.2.2.28 created 2009
Orthology
K03649  double-stranded uracil-DNA glycosylase
Genes
ECO: b3068(mug)
ECJ: JW3040(ygjF)
ECD: ECDH10B_3243(mug)
EBW: BWG_2779(mug)
ECOK: ECMDS42_2537(ygjF)
ECE: Z4421(ygjF)
ECS: ECs3951
ECF: ECH74115_4381(mug)
ETW: ECSP_4042(mug)
ELX: CDCO157_3692
EOJ: ECO26_4170(ygjF)
EOI: ECO111_3891(ygjF)
EOH: ECO103_3748(ygjF)
ECOO: ECRM13514_3963(ygjF)
ECOH: ECRM13516_3831(ygjF)
EOK: G2583_3792(mug)
ESO: O3O_22035
ESM: O3M_03650
ESL: O3K_03610
ECW: EcE24377A_3533(mug)
ELH: ETEC_3339
ECC: c3822(ygjF)
ECP: ECP_3158
ECI: UTI89_C3506(ygjF)
ECV: APECO1_3347(ygjF)
ECX: EcHS_A3249(mug)
ECM: EcSMS35_3362(mug)
ECY: ECSE_3350
ECR: ECIAI1_3216(ygjF)
ECQ: ECED1_3737(ygjF)
ECK: EC55989_3483(ygjF)
ECT: ECIAI39_3565(ygjF)
EOC: CE10_3598(mug)
EUM: ECUMN_3552(ygjF)
ECZ: ECS88_3466(ygjF)
ELO: EC042_3362(mug)
ESE: ECSF_2912
EBR: ECB_02938(ygjF)
EBD: ECBD_0673
EKF: KO11_07375(mug)
EAB: ECABU_c34880(mug)
EDJ: ECDH1ME8569_2964(ygjF)
EIH: ECOK1_3500(mug)
ENA: ECNA114_3161(ygjF)
ELW: ECW_m3335(mug)
ELL: WFL_16300(mug)
ELC: i14_3516(ygjF)
ELD: i02_3516(ygjF)
ELP: P12B_c3164(ygjF)
EBL: ECD_02938(mug)
EBE: B21_02888(mug)
ELF: LF82_1411(mug)
ECOI: ECOPMV1_03386(mug)
ECOJ: P423_17335
ECOS: EC958_3474(ygjF)
EFE: EFER_3010(ygjF)
EAL: EAKF1_ch2860(mug)
STY: STY3391(mug)
STT: t3132(mug)
STM: STM3212(mug)
SEO: STM14_3889(mug)
SEY: SL1344_3186(mug)
SEJ: STMUK_3201(mug)
SEB: STM474_3366(mug)
SEF: UMN798_3494(mug)
SENR: STMDT2_31061(mug)
SEND: DT104_32091(mug)
SENI: CY43_16745
SPT: SPA3080(mug)
SEK: SSPA2877
SEI: SPC_3288(mug)
SEC: SCH_3159(mug)
SHB: SU5_03708
SENS: Q786_15665
SED: SeD_A3568
SEG: SG3109(mug)
SEL: SPUL_3225(mug)
SEGA: SPUCDC_3211(mug)
SET: SEN3055(mug)
SENA: AU38_15545
SENO: AU37_15550
SENV: AU39_15550
SENQ: AU40_17520
SENL: IY59_16150
SEEP: I137_15385
SENE: IA1_15535
SBG: SBG_2806(mug)
SBZ: A464_3248
SFV: SFV_3109(ygjF)
SFE: SFxv_3415(mug)
SFN: SFy_4435
SFS: SFyv_4512
SFT: NCTC1_03367(mug)
SSN: SSON_3206(ygjF)
SBO: SBO_2927(ygjF)
SBC: SbBS512_E3504(mug)
SDY: SDY_3252(ygjF)
ENC: ECL_04400
EEC: EcWSU1_03859(mug)
ECLX: LI66_19455
ECLY: LI62_21415
ECLZ: LI64_18445
ESA: ESA_00353
CSK: ES15_0625
CTU: CTU_35350(mug)
KPN: KPN_03475(ygjF)
KPU: KP1_4767(ygjF)
KPP: A79E_0643
KPE: KPK_0643(mug)
KPR: KPR_4510(mug)
KPJ: N559_0699
KPX: PMK1_00951(mug)
KPNU: LI86_03385
KPNK: BN49_0631(mug)
KVA: Kvar_0619
KOX: KOX_03145
KOE: A225_5080
EAE: EAE_03695
EAR: CCG33247
CKO: CKO_04467
CRO: ROD_36201(mug)
CAMA: F384_24895
EBT: EBL_c05310(mug)
EBF: D782_0625
PSTS: E05_34590
SPE: Spro_4301
SRL: SOD_c41080(mug)
SPLY: Q5A_022420(mug)
SMAF: D781_4023
SMW: SMWW4_v1c42250(mug)
SMAR: SM39_3729(mug)
SMAC: SMDB11_3534(mug)
SERF: L085_06910
RAA: Q7S_02760
ECA: ECA0679(mug)
PATR: EV46_02920
PATO: GZ59_05820(mug)
PCT: PC1_0547
PEC: W5S_0683
SOD: Sant_3628(mug)
ETA: ETA_04140(mug)
EPY: EpC_04170(mug)
EPR: EPYR_00435(ygjF)
EAM: EAMY_0414(ygjF)
EAY: EAM_3006(mug)
EBI: EbC_38810(mug)
ERJ: EJP617_15680(mug)
EGE: EM595_0519(mug)
PAM: PANA_3391(mug)
PLF: PANA5342_0667(mug)
PAJ: PAJ_2636(mug)
PVA: Pvag_2669(mug)
PSTW: DSJ_20610
ETR: ETAE_0453(mug)
ETD: ETAF_0406
ETE: ETEE_2213
PFS: PFLU_2379(mug)
PFC: PflA506_2256(mug)
PMAN: OU5_1114
PKC: PKB_2340
PANR: A7J50_3346
TCX: Tcr_1313
CYQ: Q91_0179
SVA: SVA_0266
BCN: Bcen_2162
BCJ: BCAL0828
BCEO: I35_2843
BCT: GEM_0665
BCED: DM42_2294(mug)
BCON: NL30_26970
BTEI: WS51_24235
BSEM: WJ12_13890
BMEC: WJ16_14145
BGO: BM43_1562
BPH: Bphy_5004
BFN: OI25_4835
JAG: GJA_152(mug)
BBAG: E1O_06260
NEU: NE1560
MFA: Mfla_2486
MXA: MXAN_2371(mug)
CCX: COCOR_05696(mug)
SCL: sce5098
PLA: Plav_2151
ARA: Arad_0493
RLE: pRL110472
RLG: Rleg_6655
BJA: blr6661
BRA: BRADO5719
BBT: BBta_6231
BRS: S23_16490
AOL: S58_18950
BRAD: BF49_4989
RPA: RPA3830
RPT: Rpal_4358
BID: Bind_1119
MMED: Mame_03384(mug)
CAK: Caul_3568
RSU: NHU_01003(mug)
HNE: HNE_2611
BMQ: BMQ_2482
BMD: BMD_2459
BMH: BMWSH_2730(mug)
BMEG: BG04_4862
BEO: BEH_15000
OIH: OB1065
PPY: PPE_01010
PPM: PPSC2_05265(ygjF)
PPO: PPM_0992(ygjF)
PPOL: X809_05165
PPQ: PPSQR21_010600(mug)
PPOY: RE92_06620
PSAB: PSAB_04625
PRI: PRIO_1149
AMT: Amet_1297
CCE: Ccel_2870
STH: STH940
SAY: TPY_3757
MYO: OEM_29780
MCHE: BB28_24440
MSTE: MSTE_04954(mug)
CDI: DIP0828
CDP: CD241_0742(mug)
CDH: CDB402_0715(mug)
CDT: CDHC01_0741(mug)
CDE: CDHC02_0742(mug)
CDR: CDHC03_0741(mug)
CDA: CDHC04_0749(mug)
CDZ: CD31A_0839(mug)
CDB: CDBH8_0785(mug)
CDS: CDC7B_0749(mug)
CDD: CDCE8392_0740(mug)
CDW: CDPW8_0800(mug)
CDV: CDVA01_0708(mug)
CDIP: ERS451417_00735(mug)
CPL: Cp3995_0646(mug)
CPG: Cp316_0658(mug)
CPP: CpP54B96_0647(mug)
CPK: Cp1002_0636(mug)
CPQ: CpC231_0635(mug)
CPX: CpI19_0635(mug)
CPZ: CpPAT10_0636(mug)
COR: Cp267_0665(mug)
COP: Cp31_0642(mug)
COD: Cp106_0620(mug)
COS: Cp4202_0629(mug)
COI: CpCIP5297_0647(mug)
COE: Cp258_0641(mug)
COU: Cp162_0634(mug)
CPSE: CPTA_01187
CPSU: CPTB_00345
CPSF: CPTC_01178
CUN: Cul210932_0715(mug)
CUS: CulFRC11_0684(mug)
CUQ: Cul210931_0683(mug)
CUZ: Cul05146_0735(mug)
CUJ: CUL131002_0692c(mug)
CVA: CVAR_1894
CMQ: B840_05255(mug)
CGV: CGLAU_05540(mug)
CAMG: CAMM_08665
NFA: NFA_28360
NFR: ERS450000_01253(mug)
RER: RER_42130(mug)
REY: O5Y_19705
ROP: ROP_59560(mug)
REQ: REQ_13500
RRT: 4535765_01309(mug)
GPO: GPOL_174p00650(mug)
GOR: KTR9_2659
TPR: Tpau_3921
SCO: SCO1255(2SCG18.02)
SALB: XNR_5549
SMA: SAVERM_7076(mug)
SGR: SGR_6271
SGB: WQO_04250
SCB: SCAB_78041(mug)
SCT: SCAT_0358(mug)
SFA: Sfla_5565
SBH: SBI_08849(mug)
SHY: SHJG_2693
SVE: SVEN_0854
STRP: F750_1042
SFI: SFUL_813
SALU: DC74_7203
SALL: SAZ_37350
STRE: GZL_01444
SLD: T261_0562
STRM: M444_28550
SAMB: SAM23877_1342(mug)
SPRI: SPRI_6277
SRW: TUE45_01649(mug)
SLE: sle_58130(sle_58130)
SRN: A4G23_00567(mug)
STRD: NI25_32980
SMAL: SMALA_6851
SLAU: SLA_0758
SALJ: SMD11_5824
SLX: SLAV_31205(mug)
KSK: KSE_32500(mug)
MTS: MTES_0138
KRH: KRH_07890(mug)
SERJ: SGUI_3266
BLIN: BLSMQ_1597
PFR: PFREUD_00870(mug)
PFRE: RM25_2186
MPH: MLP_31410(mug)
NCA: Noca_3712
NDK: I601_0410(mug_1) I601_1929(mug_2)
KFL: Kfla_2488
PSIM: KR76_06000
TFU: Tfu_1918
NDA: Ndas_1812
NAL: B005_1775
TCU: Tcur_1752
SRO: Sros_6749
FAL: FRAAL1075(mug)
NML: Namu_0939
GOB: Gobs_2271
BSD: BLASA_2939(mug)
MMAR: MODMU_2251
SEN: SACE_4427(mug)
AMD: AMED_9194
AMN: RAM_47155
AMM: AMES_9057
AMZ: B737_9058
AOI: AORI_7932
AMQ: AMETH_6936(mug)
PDX: Psed_4717
PSEA: WY02_24165
PSEE: FRP1_18625
PSEH: XF36_07285
AMI: Amir_1727
SESP: BN6_25200
KAL: KALB_3346
MIL: ML5_4002
ASE: ACPL_1892(mug)
ACTN: L083_2202(mug)
AFS: AFR_10840
ACTS: ACWT_1770
CAI: Caci_4484
AHE: Arch_1480
BLN: Blon_0119
BLON: BLIJ_0120
BLL: BLJ_0088
BSCA: BBSC_0133
AYM: YM304_28680(mug)
LBO: LBWT_4530
CTHE: Chro_5488
STI: Sthe_3290
CAP: CLDAP_39500(mug)
DRA: DR_0715
DDR: Deide_17530(mug)
DGO: DGo_CA1116(mug)
GES: VT84_16430(mug)
KST: KSMBR1_1776(mug)
GAU: GAU_3910(mug)
GBA: J421_1568
CPI: Cpin_6125
NDE: NIDE0317(mug)
NMV: NITMOv2_0234(mug)
NJA: NSJP_1367(mug)
MBU: Mbur_0989
MHU: Mhun_1017
 » show all
Taxonomy
Reference
1  [PMID:10581234]
  Authors
Barrett TE, Scharer OD, Savva R, Brown T, Jiricny J, Verdine GL, Pearl LH
  Title
Crystal structure of a thwarted mismatch glycosylase DNA repair complex.
  Journal
EMBO J 18:6599-609 (1999)
DOI:10.1093/emboj/18.23.6599
  Sequence
[eco:b3068]
Reference
2  [PMID:10956012]
  Authors
Sung JS, Mosbaugh DW
  Title
Escherichia coli double-strand uracil-DNA glycosylase: involvement in uracil-mediated DNA base excision repair and stimulation of activity by endonuclease IV.
  Journal
Biochemistry 39:10224-35 (2000)
DOI:10.1021/bi0007066
Reference
3  [PMID:11555290]
  Authors
Mokkapati SK, Fernandez de Henestrosa AR, Bhagwat AS
  Title
Escherichia coli DNA glycosylase Mug: a growth-regulated enzyme required for mutation avoidance in stationary-phase cells.
  Journal
Mol Microbiol 41:1101-11 (2001)
DOI:10.1046/j.1365-2958.2001.02559.x
  Sequence
[eco:b3068]
Other DBs
ExplorEnz - The Enzyme Database: 3.2.2.28
IUBMB Enzyme Nomenclature: 3.2.2.28
ExPASy - ENZYME nomenclature database: 3.2.2.28
BRENDA, the Enzyme Database: 3.2.2.28

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