KEGG   ENZYME: 3.2.2.8Help
Entry
EC 3.2.2.8                  Enzyme                                 

Name
ribosylpyrimidine nucleosidase;
N-ribosylpyrimidine nucleosidase;
pyrimidine nucleosidase;
N-ribosylpyrimidine ribohydrolase;
pyrimidine nucleoside hydrolase;
RihB;
YeiK;
nucleoside ribohydrolase
Class
Hydrolases;
Glycosylases;
Hydrolysing N-glycosyl compounds
BRITE hierarchy
Sysname
pyrimidine-nucleoside ribohydrolase
Reaction(IUBMB)
a pyrimidine nucleoside + H2O = D-ribose + a pyrimidine base [RN:R02172]
Reaction(KEGG)
Substrate
pyrimidine nucleoside [CPD:C03169];
H2O [CPD:C00001]
Product
D-ribose [CPD:C00121];
pyrimidine base [CPD:C00396]
Comment
Also hydrolyses purine D-ribonucleosides, but much more slowly. 2'-, 3'- and 5'-deoxynucleosides are not substrates [3].
History
EC 3.2.2.8 created 1972
Pathway
ec00230  Purine metabolism
ec00240  Pyrimidine metabolism
ec01100  Metabolic pathways
Orthology
K10213  ribosylpyrimidine nucleosidase
Genes
ECO: b2162(rihB)
ECJ: JW2149(rihB)
ECD: ECDH10B_2319(rihB)
EBW: BWG_1944(rihB)
ECOK: ECMDS42_1731(rihB)
ECE: Z3419(yeiK)
ECS: ECs3054(rihB)
ECF: ECH74115_3298
ETW: ECSP_3040(rihB)
ELX: CDCO157_2817(rihB)
EOJ: ECO26_3074(rihB)
EOI: ECO111_2880(rihB)
EOH: ECO103_2637(rihB)
ECOO: ECRM13514_2924(rihB)
ECOH: ECRM13516_2864(rihB)
ECG: E2348C_2308(rihB)
EOK: G2583_2705(rihB)
ELR: ECO55CA74_13375(rihB)
ESO: O3O_17000(rihB)
ESM: O3M_08585(rihB)
ESL: O3K_08635(rihB)
ELH: ETEC_2297
ECC: c2696(yeiK)
ECP: ECP_2202
ECI: UTI89_C2436(yeiK)
ECV: APECO1_43912(rihB)
ECY: ECSE_2430
ECR: ECIAI1_2242(rihB)
ECQ: ECED1_2610(rihB)
ECK: EC55989_2415(rihB)
ECT: ECIAI39_2302(rihB)
EOC: CE10_2534(rihB)
EUM: ECUMN_2498(rihB)
ECZ: ECS88_2310(rihB)
ELO: EC042_2395(rihB)
ELN: NRG857_10975(rihB)
ESE: ECSF_2043
EBR: ECB_02091(rihB)
EBD: ECBD_1496
EKF: KO11_11820(rihB)
EAB: ECABU_c24930(rihB)
EDJ: ECDH1ME8569_2098(rihB)
ENA: ECNA114_2253(yeiK)
ELU: UM146_05985(rihB)
ELW: ECW_m2363(rihB)
ELL: WFL_11560(rihB)
ELC: i14_2497(rihB)
ELD: i02_2497(rihB)
ELP: P12B_c2256(rihB)
EBL: ECD_02091(rihB)
EBE: B21_02050(rihB)
ELF: LF82_1884(rihB)
ECOA: APECO78_14530(rihB)
ECOL: LY180_11300(rihB)
ECOI: ECOPMV1_02322(rihB)
ECOJ: P423_12155(rihB)
ECOS: EC958_2498(yeiK)
EFE: EFER_2248(rihB)
EMA: C1192_01120(rihB)
SFL: SF2247(rihB)
SFX: S2376(yeiK)
SFV: SFV_2237(yeiK)
SFE: SFxv_2480(rihB)
SFN: SFy_3191
SFS: SFyv_3266
SFT: NCTC1_02470(rihB)
SSN: SSON_2218(yeiK)
SBO: SBO_2165(yeiK)
SDY: SDY_2104(yeiK)
SHQ: A0259_14630(rihB)
HAV: AT03_11825(rihB)
OPO: DSM2777_15880(rihB)
LRI: NCTC12151_01991(rihB)
LBR: LVIS_2188
CEU: A7L45_04060(rihB)
RUM: CK1_26280
LACY: A4V08_10600(rihB)
OVA: OBV_11660(rihB)
MBJ: KQ51_00964(rihB_1)
AMQ: AMETH_4651(ybeK)
BDE: BDP_1920(iunH)
BDN: BBDE_1812
BRM: Bmur_2117
BPW: WESB_0409
LBA: Lebu_1571
LEO: AMK43_06395(rihB) AMK43_06435(rihB)
TAI: Taci_0564
 » show all
Taxonomy
Reference
1  [PMID:12325375]
  Authors
Terada M, Tatibana M, Hayaishi O.
  Title
Purification and properties of nucleoside hydrolase from Pseudomonas fluorescens.
  Journal
J Biol Chem 242:5578-85 (1967)
Reference
2  [PMID:11027694]
  Authors
Petersen C, Moller LB.
  Title
The RihA, RihB, and RihC ribonucleoside hydrolases of Escherichia coli. Substrate specificity, gene expression, and regulation.
  Journal
J Biol Chem 276:884-94 (2001)
DOI:10.1074/jbc.M008300200
  Sequence
[eco:b2162]
Reference
3  [PMID:14993681]
  Authors
Giabbai B, Degano M.
  Title
Cloning, purification, crystallization and X-ray analysis of the Escherichia coli pyrimidine nucleoside hydrolase YeiK.
  Journal
Acta Crystallogr D Biol Crystallogr 60:524-7 (2004)
DOI:10.1107/S0907444903028488
Reference
4  [PMID:15130467]
  Authors
Giabbai B, Degano M.
  Title
Crystal structure to 1.7 a of the Escherichia coli pyrimidine nucleoside hydrolase YeiK, a novel candidate for cancer gene therapy.
  Journal
Structure 12:739-49 (2004)
DOI:10.1016/j.str.2004.03.018
  Sequence
[eco:b2162]
Other DBs
ExplorEnz - The Enzyme Database: 3.2.2.8
IUBMB Enzyme Nomenclature: 3.2.2.8
ExPASy - ENZYME nomenclature database: 3.2.2.8
BRENDA, the Enzyme Database: 3.2.2.8
CAS: 37288-60-1

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