KEGG   ENZYME: 3.4.17.21Help
Entry
EC 3.4.17.21                Enzyme                                 

Name
glutamate carboxypeptidase II;
N-acetylated-gamma-linked-acidic dipeptidase (NAALADase);
folate hydrolase;
prostate-specific membrane antigen;
pteroylpoly-gamma-glutamate carboxypeptidase;
microsomal gamma-glutamyl carboxypeptidase;
pteroylpolyglutamate hydrolase;
folylpolyglutamate hydrolase;
pteroylpoly-gamma-glutamate hydrolase;
pteroylpolygammaglutamyl hydrolase;
pteroylpolyglutamate hydrolase;
pteroylpolyglutamic acid hydrolase;
PSM antigen;
acetylaspartylglutamate dipeptidase;
NAALA dipeptidase;
rat NAAG peptidase;
mGCP;
membrane glutamate carboxypeptidase;
N-acetylated-alpha-linked-amino dipeptidase;
prostrate-specific membrane antigen;
N-Acetylated alpha-linked acidic dipeptidase;
PSMA
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Metallocarboxypeptidases
BRITE hierarchy
Reaction(IUBMB)
Release of an unsubstituted, C-terminal glutamyl residue, typically from Ac-Asp-Glu or folylpoly-gamma-glutamates [RN:R10687]
Reaction(KEGG)
R10687;
(other) R10688
Show
Comment
A metallo-carboxypeptidase that is predominantly expressed as a membrane-bound enzyme of 94-100 kDa , but also exists in a soluble form. Hydrolyses alpha-peptide bonds in Ac-Asp-Glu, Asp-Glu, and Glu-Glu, but also gamma-glutamyl bonds in gamma-Glu-Glu, and folylpoly-gamma-glutamates. With folylpoly-gamma-glutamates, shows processive carboxypeptidase activity to produce pteroylmonoglutamate [4]. Does not hydrolyse Ac-beta-Asp-Glu. Known inhibitors: quisqualic acid, Ac-beta-Asp-Glu, and 2-phosphonomethyl-pentanedioate. In peptidase family M28 of Vibrio leucyl aminopeptidase. The release of C-terminal glutamate from folylpoly-gamma-glutamates is also catalysed by EC 3.4.17.11 (glutamate carboxypeptidase) and EC 3.4.19.9 (gamma-Glu-X carboxypeptidase).
History
EC 3.4.17.21 created 1997, modified 2000 (EC 3.4.13.8 created 1972 and EC 3.4.19.8 created 1992, incorporated 2000)
Pathway
ec00250  Alanine, aspartate and glutamate metabolism
ec01100  Metabolic pathways
Orthology
K01301  N-acetylated-alpha-linked acidic dipeptidase
K14592  glutamate carboxypeptidase II (folate hydrolase 1)
Genes
HSA: 10003(NAALAD2) 10004(NAALADL1) 2346(FOLH1) 254827(NAALADL2)
PTR: 451185 451309(NAALADL1) 451475(FOLH1B) 451478(NAALAD2) 745481(NAALADL2)
PPS: 100970655(NAALAD2) 100970744(NAALADL1) 100980316 100984915 100991481(NAALADL2)
GGO: 101124045(NAALAD2) 101133710(NAALADL1) 101136594(NAALADL2) 101147888(FOLH1)
PON: 100445995(NAALADL1) 100448157 100453848(FOLH1B) 100460916(NAALAD2) 103890310(NAALADL2)
NLE: 100579763(NAALADL1) 100580501 100582294(NAALAD2) 100582881(NAALADL2) 100589099(FOLH1B) 100600258
MCC: 694180(NAALAD2) 707714(FOLH1B) 717018(NAALADL1) 721738(NAALADL2)
MCF: 101866140(FOLH1B) 101926468(NAALAD2) 102124059(NAALADL1) 102142872 102145366 102146742(NAALADL2)
CSAB: 103221229(NAALADL2) 103231779(NAALADL1) 103248197(FOLH1B) 103248216(NAALAD2)
RRO: 104658723 104658729 104660791 104662834(NAALADL1) 104672807(NAALAD2) 104678909(FOLH1B)
RBB: 108512432(NAALADL2) 108519803(NAALAD2) 108539153(FOLH1B) 108543140(NAALADL1)
CJC: 100393124(NAALADL2) 100396945(FOLH1B) 100406630(NAALAD2) 100413235(NAALADL1)
SBQ: 101032246(NAALADL1) 101032598(NAALADL2) 101033656(NAALAD2) 101044541(FOLH1B)
MMU: 381204(Naaladl1) 53320(Folh1) 635702(Naaladl2) 72560(Naalad2)
RNO: 300384(Naalad2) 499583(Naaladl2) 83568(Naaladl1) 85309(Folh1)
CGE: 100761363(Naaladl2) 100768106(Naaladl1) 100768684 100769032 103159483
NGI: 103728962(Naaladl2) 103737918(Naalad2) 103738766(Naaladl1) 103750517(Folh1)
HGL: 101702083(Naaladl1) 101707025(Naaladl2) 101722660(Naalad2) 101725016(Folh1b)
CCAN: 109679622 109687101(Naaladl1) 109696834 109699708(Naalad2)
OCU: 100339446(NAALAD2) 100342215(NAALADL2) 100358060(FOLH1) 103346158(NAALADL1)
TUP: 102475075(NAALADL1) 102486443 102487205(FOLH1B) 102488479(NAALAD2) 102499204(NAALADL2) 102499846
CFA: 476775(FOLH1) 483751(NAALADL1) 485139 488174(NAALADL2)
AML: 100469236(NAALAD2) 100470423(NAALADL1) 100472805(FOLH1B) 100482558(NAALADL2)
UMR: 103660690(NAALAD2) 103675371(FOLH1B) 103676133(NAALADL2) 103679566(NAALADL1)
FCA: 101082777(NAALADL1) 101089389(NAALAD2) 101089641(FOLH1B) 101098650(NAALADL2)
PTG: 102953631(NAALADL1) 102957765(FOLH1B) 102958523(NAALAD2) 102970620(NAALADL2)
AJU: 106971153(NAALADL1) 106973866(NAALADL2) 106983063(FOLH1B) 106983075(NAALAD2) 106990083
BTA: 100337356(NAALADL2) 505865(FOLH1B) 506692(NAALADL1) 536439(NAALAD2)
BOM: 102265480(NAALAD2) 102266817(NAALADL2) 102272952(FOLH1B) 102284550 102286181(NAALADL1) 106700854
BIU: 109554530(NAALADL1) 109554596(FOLH1B) 109554645(NAALAD2) 109557961(NAALADL2)
PHD: 102315808(NAALADL1) 102320530(NAALAD2) 102323016(FOLH1B) 102326158(NAALADL2)
CHX: 102170637(NAALADL2) 102177776(NAALAD2) 102186842(NAALADL1) 102190665(FOLH1B)
OAS: 101105198(NAALADL1) 101111764(NAALADL2) 101120451(NAALAD2) 101121723(FOLH1B)
SSC: 100515250(NAALADL1) 100522279(NAALADL2) 100524386(NAALAD2) 397677(FOLH1B)
CFR: 102510657(FOLH1B) 102511847(NAALADL2) 102519706(NAALADL1) 102520257(NAALAD2) 106730792
CDK: 105085936(NAALADL2) 105088594(FOLH1B) 105088596(NAALAD2) 105102621(NAALADL1)
BACU: 103001153 103002601(NAALAD2) 103012348(FOLH1B) 103019730(NAALADL1)
LVE: 103072597(NAALADL1) 103079394(NAALADL2) 103089873 103090789(NAALAD2)
OOR: 101273529(NAALADL2) 101277636 101280978 101281334 101283878(NAALADL1)
ECB: 100050187(NAALAD2) 100056368(NAALADL1) 100060132(FOLH1B) 100064889(NAALADL2)
EPZ: 103547857(FOLH1B) 103552273(NAALADL2) 103558584(NAALAD2) 103559154(NAALADL1)
EAI: 106822677(FOLH1B) 106827519(NAALAD2) 106833668(NAALADL1) 106847520(NAALADL2)
MYB: 102246530(NAALADL1) 102253215(NAALADL2) 102258767(FOLH1B) 102262061(NAALAD2)
MYD: 102757107 102757990(NAALADL2) 102761189(FOLH1B) 102761368(NAALAD2) 102774994(NAALADL1) 107181485
HAI: 109373422(NAALADL2) 109374031(NAALAD2) 109374041(FOLH1B) 109384301(NAALADL1)
RSS: 109450479(NAALADL1) 109451367(NAALAD2) 109451413(FOLH1B) 109458520(NAALADL2)
PALE: 102888077(NAALADL2) 102893898(NAALADL1) 102895068 102895568
LAV: 100660135(FOLH1B) 100664500(NAALADL1) 100667240(NAALADL2) 100677198(NAALAD2)
MDO: 100009990(FOLH1B) 100011662(NAALADL2) 100013563(NAALAD2) 100030670(NAALADL1)
SHR: 100913549(NAALADL1) 100918727 100927408(NAALAD2) 100930261(NAALADL2)
GGA: 419010(NAALAD2) 419011(FOLH1) 429156(NAALADL2)
MGP: 100549526(NAALADL2) 100550377 100550530(NAALAD2) 100550836
CJO: 107308460 107308462 107318243(NAALADL2)
APLA: 101789505(NAALAD2) 101790444(FOLH1B) 101798205 101798312(NAALADL2)
ACYG: 106032006(NAALADL2) 106034162 106034164
TGU: 100219205(NAALADL2) 100223080 100225953(NAALAD2)
GFR: 102034713(NAALAD2) 102034877(FOLH1B) 102042454(NAALADL2)
FAB: 101814982(FOLH1) 101815312(NAALAD2) 101818062(NAALADL2)
PHI: 102104341(NAALADL2) 102105283(FOLH1B) 102106596(NAALAD2)
PMAJ: 107205820 107208885(NAALADL2) 107211745
CCAE: 111924890 111933596(NAALADL2)
CCW: 104688028(NAALADL2) 104690325 104690326
FPG: 101911476(NAALADL2) 101915194(NAALAD2) 101918547
FCH: 102051438(NAALADL2) 102054726(NAALAD2) 102058819(FOLH1B)
CLV: 102092293(NAALADL2) 102094196 102095234
EGZ: 104126200(NAALADL2) 104128837 104128839
ASN: 102373311(NAALADL2) 102373839 102381492(NAALADL1) 102387337 102387587
AMJ: 102562464(NAALADL1) 102567523(NAALADL2) 102569346(FOLH1B) 106737083
PSS: 102454431(NAALADL1) 102454607(NAALADL2) 102455710(FOLH1B) 102456275
CMY: 102930770(NAALADL2) 102933727(NAALAD2) 102934118(FOLH1B) 102942487(NAALADL1)
CPIC: 101933817(NAALAD2) 101934265 101939871(NAALADL1) 101948732(NAALADL2) 101952117
ACS: 100551729 100551830(naaladl1) 100552710(naaladl2) 100564848(naalad2)
XLA: 100036903(naaladl1.L) 100037180 108707586 108709033 108717004(naaladl2.L) 734632(naalad2.L)
XTR: 100485577(folh1) 100487005 100487953(naaladl1) 100498087(naaladl2) 101731087 594947(naalad2)
NPR: 108785259(NAALADL1) 108794765(FOLH1B) 108794950(NAALADL2) 108795527 108802840
DRE: 100000223(si:ch211-214j8.1) 101885785 393247(naalad2)
SRX: 107744479(naaladl1) 107753869(naaladl2)
SANH: 107655960(naaladl2) 107673364(naaladl1) 107674502 107674503
IPU: 108255540(naaladl1) 108255541 108271148(naaladl2)
AMEX: 103021590 103025360 103033129(naaladl1) 103041756(folh1b)
TRU: 101065215(folh1b) 101077227(naaladl1) 105418700
LCO: 104920121(folh1b) 104928492(naaladl1) 109137913(naaladl2)
NCC: 104950428(naaladl1) 104951797
MZE: 101473097(naaladl1) 101476953 101487368(naaladl2) 106676838
XMA: 102216435(naaladl2) 102229486(naaladl1) 102230464(folh1b)
PRET: 103464027(naaladl2) 103469958(naaladl1) 103474484(folh1b)
NFU: 107380456(folh1b) 107387348(naaladl2) 107394320(naaladl1)
KMR: 108233753(naaladl1) 108244651(folh1b)
CSEM: 103378545(folh1b) 103397950(naaladl1)
LCF: 108878813(naaladl2) 108881008(naaladl1)
SDU: 111224120(folh1b) 111237749(naaladl1)
HCQ: 109510817 109525183(naaladl1) 109525428(folh1b)
MALB: 109959081(naaladl1) 109971604(folh1b)
SASA: 106570846(naaladl1) 106581411 106613925(naaladl2)
OTW: 112251038(naaladl2) 112262525(naaladl1)
ELS: 105021665(naaladl1) 105024062(folh1b) 105025892(naaladl2)
SFM: 108932227(folh1b) 108937262(naaladl2) 108939004(naaladl1)
LCM: 102346345(NAALADL1) 102356080 102361235 102366958(FOLH1B)
CMK: 103175760(naaladl2) 103184309
ZNE: 110836776
TUT: 107366146
CEL: CELE_R57.1(gcp-2.1)
SHX: MS3_00248
EGL: EGR_00432
CPAP: 110806397
LJA: Lj1g3v4763600.1(Lj1g3v4763600.1) Lj1g3v4763600.2(Lj1g3v4763600.2) Lj2g3v0878750.1(Lj2g3v0878750.1) Lj2g3v0878750.2(Lj2g3v0878750.2) Lj2g3v0878930.1(Lj2g3v0878930.1) Lj2g3v0878930.2(Lj2g3v0878930.2) Lj2g3v0889170.1(Lj2g3v0889170.1) Lj2g3v0889190.1(Lj2g3v0889190.1) Lj2g3v0889190.2(Lj2g3v0889190.2)
LANG: 109334865
CSV: 101221361
CMO: 103486721
CMAX: 111498787
CMOS: 111446753
CPEP: 111800317
BVG: 104889147
SOE: 110776101
DOSA: Os01t0740500-00(Os01g0740500) Os01t0740650-00(Os01g0740650) Os01t0743300-01(Os01g0743300) Os03t0790600-01(Os03g0790600)
ATS: 109757082(LOC109757082) 109765341(LOC109765341) 109767415(LOC109767415) 109775216(LOC109775216) 109775219(LOC109775219)
ZMA: 100147738(vp8) 100280068 100280279(cl4954_1) 100280900
ERC: Ecym_1131
KMX: KLMA_40518(VPS70) KLMA_50496(VPS70)
NCS: NCAS_0J02130(NCAS0J02130)
NDI: NDAI_0J02840(NDAI0J02840)
TPF: TPHA_0C00760(TPHA0C00760) TPHA_0K00560(TPHA0K00560)
TBL: TBLA_0A04020(TBLA0A04020) TBLA_0B06200(TBLA0B06200)
TDL: TDEL_0D00700(TDEL0D00700)
KAF: KAFR_0E04260(KAFR0E04260)
PIC: PICST_33956(VPS70) PICST_72031(GCP1)
CAL: CAALFM_C700170WA(VPS70)
SLB: AWJ20_4763(VPS70)
NCR: NCU03108
NTE: NEUTE1DRAFT126170(NEUTE1DRAFT_126170)
SSCK: SPSK_03979
MAW: MAC_07416
MAJ: MAA_00651
ANI: AN4018.2
ANG: ANI_1_512164(An18g03980)
CNB: CNBA2810
ABP: AGABI1DRAFT106593(AGABI1DRAFT_106593) AGABI1DRAFT72743(AGABI1DRAFT_72743)
ABV: AGABI2DRAFT146446(AGABI2DRAFT_146446) AGABI2DRAFT219485(AGABI2DRAFT_219485)
MGL: MGL_4102
SPAR: SPRG_00397
SEN: SACE_6113
GLJ: GKIL_2068
SACI: Sinac_6856
ACA: ACP_0923
SUS: Acid_4582
GAU: GAU_0163
RMR: Rmar_2092
CPI: Cpin_4009
FLN: FLA_5821
MUC: MuYL_0306
DFE: Dfer_1367
SLI: Slin_0518
 » show all
Taxonomy
Reference
1  [PMID:9123729]
  Authors
Heston WD.
  Title
Characterization and glutamyl preferring carboxypeptidase function of prostate specific membrane antigen: a novel folate hydrolase.
  Journal
Urology 49:104-12 (1997)
DOI:10.1016/S0090-4295(97)00177-5
Reference
2  [PMID:9187245]
  Authors
Rawlings ND, Barrett AJ.
  Title
Structure of membrane glutamate carboxypeptidase.
  Journal
Biochim Biophys Acta 1339:247-52 (1997)
DOI:10.1016/S0167-4838(97)00008-3
  Sequence
[hsa:2346]
Reference
3  [PMID:9685395]
  Authors
Halsted CH, Ling EH, Luthi-Carter R, Villanueva JA, Gardner JM, Coyle JT.
  Title
Folylpoly-gamma-glutamate carboxypeptidase from pig jejunum. Molecular characterization and relation to glutamate carboxypeptidase II.
  Journal
J Biol Chem 273:20417-24 (1998)
DOI:10.1074/jbc.273.32.20417
  Sequence
[ssc:397677]
Reference
4  [PMID:9501243]
  Authors
Luthi-Carter R, Berger UV, Barczak AK, Enna M, Coyle JT.
  Title
Isolation and expression of a rat brain cDNA encoding glutamate carboxypeptidase II.
  Journal
Proc Natl Acad Sci U S A 95:3215-20 (1998)
DOI:10.1073/pnas.95.6.3215
  Sequence
[rno:85309]
Other DBs
ExplorEnz - The Enzyme Database: 3.4.17.21
IUBMB Enzyme Nomenclature: 3.4.17.21
ExPASy - ENZYME nomenclature database: 3.4.17.21
BRENDA, the Enzyme Database: 3.4.17.21
CAS: 9074-87-7

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