KEGG   ENZYME: 3.4.21.104Help
Entry
EC 3.4.21.104               Enzyme                                 

Name
mannan-binding lectin-associated serine protease-2;
MASP-2;
MASP2;
MBP-associated serine protease-2;
mannose-binding lectin-associated serine protease-2;
p100;
mannan-binding lectin-associated serine peptidase 2
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Serine endopeptidases
BRITE hierarchy
Reaction(IUBMB)
Selective cleavage after Arg223 in complement component C2 (-Ser-Leu-Gly-Arg!Lys-Ile-Gln-Ile) and after Arg76 in complement component C4 (-Gly-Leu-Gln-Arg!Ala-Leu-Glu-Ile)
Comment
Mannan-binding lectin (MBL) recognizes patterns of neutral carbohydrates, such as mannose and N-acetylglucosamine, on a wide range of microbial surfaces and is able to initiate activation of the lectin pathway of complement [7]. This enzyme displays C_overbar_1s_-like esterolytic activity (cf. EC 3.4.21.42, complement subcomponent C_overbar_1s_). It also cleaves C4 and C2 with efficiencies that are relatively higher than those of EC 3.4.21.42 [3]. Belongs in peptidase family S1A.
History
EC 3.4.21.104 created 2005
Orthology
K03993  mannan-binding lectin serine protease 2
Genes
HSA: 10747(MASP2)
PTR: 107974877(MASP2)
PPS: 100981899(MASP2)
GGO: 101126677(MASP2)
PON: 100458242(MASP2)
NLE: 100593253(MASP2)
MCC: 722701 722714(MASP2)
MCF: 102131322(MASP2)
CSAB: 103225618(MASP2)
RRO: 104654364(MASP2)
RBB: 108539381(MASP2)
CJC: 100408774(MASP2)
SBQ: 101047723(MASP2)
MMU: 17175(Masp2)
RNO: 64459(Masp2)
CGE: 100756295(Masp2) 100770654
NGI: 103733193(Masp2)
HGL: 101696699(Masp2) 101706103
CCAN: 109689658(Masp2)
TUP: 102488905(MASP2)
CFA: 487447(MASP2)
AML: 100466924(MASP2)
UMR: 103666866(MASP2)
ORO: 101362776(MASP2)
FCA: 101099568(MASP2)
PTG: 102955677(MASP2)
AJU: 106984902(MASP2)
BTA: 505819(MASP2)
BOM: 102280596(MASP2)
BIU: 109570390(MASP2)
PHD: 102330968 102331327(MASP2)
CHX: 102169785(MASP2)
OAS: 101112586(MASP2)
SSC: 100302537(MASP2)
CFR: 102513488(MASP2)
CDK: 105107020(MASP2)
BACU: 103005678(MASP2)
LVE: 103083615(MASP2)
OOR: 101283337(MASP2)
ECB: 100056770(MASP2)
EPZ: 103552243(MASP2)
EAI: 106836680(MASP2)
MYD: 102764933(MASP2)
HAI: 109381284(MASP2)
RSS: 109455271(MASP2)
PALE: 102885750(MASP2)
LAV: 100662293(MASP2)
TMU: 101342871
MDO: 100018957(MASP2)
SHR: 100932261(MASP2)
OAA: 100079601(MASP2)
GGA: 407089(MASP2)
MGP: 100547191(MASP2)
CJO: 107323403(MASP2)
APLA: 101801331(MASP2)
ACYG: 106040226(MASP2)
TGU: 100232734(MASP2)
GFR: 102032737(MASP2)
PHI: 102110924(MASP2)
PMAJ: 107213694(MASP2)
CCAE: 111938694(MASP2)
CCW: 104693415(MASP2)
FPG: 101919004(MASP2)
FCH: 102051305(MASP2)
CLV: 102091890(MASP2)
EGZ: 104133133(MASP2)
AAM: 106482473(MASP2)
ASN: 102370755(MASP2)
AMJ: 102559456(MASP2)
PSS: 102453425(MASP2)
CMY: 102929438(MASP2)
CPIC: 101950973(MASP2)
PVT: 110073231(MASP2)
PBI: 103053568(MASP2)
GJA: 107124364(MASP2)
XLA: 108696362(masp2.L) 373654(masp2.S)
XTR: 496446(masp2)
DRE: 560277(masp2)
SRX: 107708846
SANH: 107681988(masp2)
SGH: 107571531
CCAR: 109048441
AMEX: 103023364(masp2)
XMA: 102236233
SASA: 106605730
SFM: 108923279(masp2)
LCM: 102360645(MASP2)
CMK: 103181367(masp2)
 » show all
Taxonomy
Reference
1  [PMID:1460414]
  Authors
Matsushita M, Fujita T.
  Title
Activation of the classical complement pathway by mannose-binding protein in association with a novel C1s-like serine protease.
  Journal
J Exp Med 176:1497-502 (1992)
Reference
2  [PMID:9087411]
  Authors
Thiel S, Vorup-Jensen T, Stover CM, Schwaeble W, Laursen SB, Poulsen K, Willis AC, Eggleton P, Hansen S, Holmskov U, Reid KB, Jensenius JC.
  Title
A second serine protease associated with mannan-binding lectin that activates complement.
  Journal
Nature 386:506-10 (1997)
DOI:10.1038/386506a0
  Sequence
[hsa:10747]
Reference
3  [PMID:11527969]
  Authors
Rossi V, Cseh S, Bally I, Thielens NM, Jensenius JC, Arlaud GJ.
  Title
Substrate specificities of recombinant mannan-binding lectin-associated serine proteases-1 and -2.
  Journal
J Biol Chem 276:40880-7 (2001)
DOI:10.1074/jbc.M105934200
Reference
4  [PMID:12538697]
  Authors
Ambrus G, Gal P, Kojima M, Szilagyi K, Balczer J, Antal J, Graf L, Laich A, Moffatt BE, Schwaeble W, Sim RB, Zavodszky P.
  Title
Natural substrates and inhibitors of mannan-binding lectin-associated serine protease-1 and -2: a study on recombinant catalytic fragments.
  Journal
J Immunol 170:1374-82 (2003)
DOI:10.4049/jimmunol.170.3.1374
Reference
5  [PMID:15364579]
  Authors
Harmat V, Gal P, Kardos J, Szilagyi K, Ambrus G, Vegh B, Naray-Szabo G, Zavodszky P.
  Title
The structure of MBL-associated serine protease-2 reveals that identical substrate specificities of C1s and MASP-2 are realized through different sets of enzyme-substrate interactions.
  Journal
J Mol Biol 342:1533-46 (2004)
DOI:10.1016/j.jmb.2004.07.014
  Sequence
[hsa:10747]
Reference
6  [PMID:15060079]
  Authors
Chen CB, Wallis R.
  Title
Two mechanisms for mannose-binding protein modulation of the activity of its associated serine proteases.
  Journal
J Biol Chem 279:26058-65 (2004)
DOI:10.1074/jbc.M401318200
Reference
7  [PMID:15728497]
  Authors
Teillet F, Dublet B, Andrieu JP, Gaboriaud C, Arlaud GJ, Thielens NM.
  Title
The two major oligomeric forms of human mannan-binding lectin: chemical characterization, carbohydrate-binding properties, and interaction with MBL-associated serine proteases.
  Journal
J Immunol 174:2870-7 (2005)
DOI:10.4049/jimmunol.174.5.2870
Other DBs
ExplorEnz - The Enzyme Database: 3.4.21.104
IUBMB Enzyme Nomenclature: 3.4.21.104
ExPASy - ENZYME nomenclature database: 3.4.21.104
BRENDA, the Enzyme Database: 3.4.21.104
CAS: 214915-16-9

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