KEGG   ENZYME: 3.4.21.111Help
Entry
EC 3.4.21.111               Enzyme                                 

Name
aqualysin 1;
caldolysin
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Serine endopeptidases
BRITE hierarchy
Reaction(IUBMB)
Exhibits low specificity towards esters of amino acids with small hydrophobic or aromatic residues at the P1 position
Comment
This enzyme from the extreme thermophile, Thermus aquaticus, is an alkaline serine peptidase. It has three subsites, S1, S2, and S3, in the substrate binding site. The preferred amino acids at the S1 site are Ala and Phe, at the S2 site are Ala and norleucine and at the S3 site are Phe and Ile [3]. These specificities are similar to those of EC 3.4.21.64 (peptidase K) and EC 3.4.21.62 (subtilisin BPN') [3]. The enzyme displays broad specificity for cleavage of insulin B-chain and hydrolyses elastin substrates such as succinyl-(Ala)n-p-nitroanilide (n = 1,2,3) and some peptide esters [1,3]. Belongs in peptidase family S8A.
History
EC 3.4.21.111 created 2006
Orthology
K20754  aqualysin 1
Genes
SUR: STAUR_7506
DGE: Dgeo_0978
DDR: Deide_09170
DMR: Deima_2317
DPT: Deipr_2239
DGO: DGo_CA0785
DSW: QR90_15910
DCH: SY84_12690
DAB: AUC44_03455
DPU: SU48_03450
TAQ: TO73_1716
MRB: Mrub_1522
 » show all
Taxonomy
Reference
1  [PMID:3162211]
  Authors
Matsuzawa H, Tokugawa K, Hamaoki M, Mizoguchi M, Taguchi H, Terada I, Kwon ST, Ohta T.
  Title
Purification and characterization of aqualysin I (a thermophilic alkaline serine protease) produced by Thermus aquaticus YT-1.
  Journal
Eur J Biochem 171:441-7 (1988)
DOI:10.1111/j.1432-1033.1988.tb13809.x
  Sequence
Reference
2  [PMID:9882104]
  Authors
Tanaka T, Matsuzawa H, Kojima S, Kumagai I, Miura K, Ohta T.
  Title
P1 specificity of aqualysin I (a subtilisin-type serine protease) from Thermus aquaticus YT-1, using P1-substituted derivatives of Streptomyces subtilisin inhibitor.
  Journal
Biosci Biotechnol Biochem 62:2035-8 (1998)
  Sequence
Reference
3  [PMID:27393587]
  Authors
Tanaka T, Matsuzawa H, Ohta T
  Title
Substrate Specificity of Aqualysin I, a Bacterial Thermophilic Alkaline Serine Protease from Thermus aquaticus YT-1: Comparison with Proteinase K, Subtilisin BPN' and Subtilisin Carlsberg.
  Journal
Biosci Biotechnol Biochem 62:2161-5 (1998)
DOI:10.1271/bbb.62.2161
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 3.4.21.111
IUBMB Enzyme Nomenclature: 3.4.21.111
ExPASy - ENZYME nomenclature database: 3.4.21.111
BRENDA, the Enzyme Database: 3.4.21.111
CAS: 88747-68-6

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