KEGG   ENZYME: 3.4.21.115Help
Entry
EC 3.4.21.115               Enzyme                                 

Name
infectious pancreatic necrosis birnavirus Vp4 peptidase;
infectious pancreatic necrosis virus protease;
IPNV Vp4 protease;
IPNV Vp4 peptidase;
NS protease;
NS-associated protease;
Vp4 protease
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Serine endopeptidases
BRITE hierarchy
Reaction(IUBMB)
Cleaves the (Ser/Thr)-Xaa-Ala!(Ser/Ala)-Gly motif in the polyprotein NH2-pVP2-VP4-VP3-COOH of infectious pancreatic necrosis virus at the pVP2-VP4 and VP4-VP3 junctions
Comment
Infectious pancreatic necrosis virus (IPNV) is a birnavirus that causes an acute, contagious disease in young salmonid fish [2]. As with most viruses that infect eukaryotic cells, the proteolytic processing of viral precursor proteins is a crucial step in the life cycle of this virus [2]. pVP2 is converted into VP2 by cleavage near the carboxy end of pVP2. This cleavage is most likely due to host-cell proteases rather than VP4 [2,3]. Differs from most serine peptidases in not having the catalytic triad Ser-His-Asp [2]. Belongs in peptidase family S50.
History
EC 3.4.21.115 created 2006
Reference
1  [PMID:2219718]
  Authors
Manning DS, Leong JC.
  Title
Expression in Escherichia coli of the large genomic segment of infectious pancreatic necrosis virus.
  Journal
Virology 179:16-25 (1990)
Reference
2  [PMID:10666235]
  Authors
Petit S, Lejal N, Huet JC, Delmas B.
  Title
Active residues and viral substrate cleavage sites of the protease of the birnavirus infectious pancreatic necrosis virus.
  Journal
J Virol 74:2057-66 (2000)
DOI:10.1128/JVI.74.5.2057-2066.2000
Reference
3
  Authors
Dobos, P.
  Title
The molecular biology of infectious pancreatic necrosis virus (IPNV).
  Journal
Annu Rev Fish Dis 5:25-54 (1995)
Other DBs
ExplorEnz - The Enzyme Database: 3.4.21.115
IUBMB Enzyme Nomenclature: 3.4.21.115
ExPASy - ENZYME nomenclature database: 3.4.21.115
BRENDA, the Enzyme Database: 3.4.21.115

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