KEGG   ENZYME: 3.4.21.63Help
Entry
EC 3.4.21.63                Enzyme                                 

Name
oryzin;
Aspergillus alkaline proteinase;
aspergillopeptidase B;
API 21;
aspergillopepsin B;
aspergillopepsin F;
Aspergillus candidus alkaline proteinase;
Aspergillus flavus alkaline proteinase;
Aspergillus melleus semi-alkaline proteinase;
Aspergillus oryzae alkaline proteinase;
Aspergillus parasiticus alkaline proteinase;
Aspergillus serine proteinase;
Aspergillus sydowi alkaline proteinase;
Aspergillus soya alkaline proteinase;
Aspergillus melleus alkaline proteinase;
Aspergillus sulphureus alkaline proteinase;
prozyme;
P 5380;
kyorinase;
seaprose S;
semi-alkaline protease;
sumizyme MP;
prozyme 10;
onoprose;
onoprose SA;
protease P;
promelase
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Serine endopeptidases
BRITE hierarchy
Reaction(IUBMB)
Hydrolysis of proteins with broad specificity, and of Bz-Arg-OEt > Ac-Tyr-OEt. Does not hydrolyse peptide amides
Comment
A peptidase of family S8 (subtilisin family), not containing cysteine, that is the predominant extracellular alkaline endopeptidase of the mold Aspergillus oryzae. Identical or closely related enzymes are produced by A. flavus and A. sojae [2,3,4]
History
EC 3.4.21.63 created 1992 (EC 3.4.21.14 created 1961 as EC 3.4.4.16, transferred 1972 to EC 3.4.21.14, modified 1986, part incorporated 1992)
Orthology
K18549  oryzin
Genes
ANI: AN5558.2
AFM: AFUA_4G11800
AOR: AO090003001036
ANG: ANI_1_1348084(An09g03780)
AFV: AFLA_027810
ACT: ACLA_050840
NFI: NFIA_104430
Taxonomy
Reference
1
  Authors
Nakagawa, Y.
  Title
Alkaline proteinases from Aspergillus.
  Journal
Methods Enzymol 19:581-591 (1970)
Reference
2
  Authors
Hayashi, K. and Terada, M.
  Title
Some characteristics of hydrolysis of synthetic substrates and proteins by the alkaline proteases from Aspergillus sojae.
  Journal
Agric Biol Chem 36:1755-1765 (1972)
Reference
3  [PMID:4623338]
  Authors
Turkova J, Mikes O, Hayashi K, Danno G, Polgar L.
  Title
Alkaline proteinases of the genus Aspergillus.
  Journal
Biochim Biophys Acta 257:257-63 (1972)
Reference
4  [PMID:4441086]
  Authors
Morihara K, Oka T, Tsuzuki H.
  Title
Comparative study of various serine alkaline proteinases from microorganisms. Esterase activity against N-acylated peptide ester substrates.
  Journal
Arch Biochem Biophys 165:72-9 (1974)
DOI:10.1016/0003-9861(74)90143-X
Reference
5  [PMID:4859351]
  Authors
Spadari S, Subramanian AR, Kalnitsky G.
  Title
Highly restricted specificity of the serine proteinase aspergillopeptidase B.
  Journal
Biochim Biophys Acta 359:267-72 (1974)
DOI:10.1016/0005-2795(74)90224-4
Other DBs
ExplorEnz - The Enzyme Database: 3.4.21.63
IUBMB Enzyme Nomenclature: 3.4.21.63
ExPASy - ENZYME nomenclature database: 3.4.21.63
BRENDA, the Enzyme Database: 3.4.21.63
CAS: 9074-07-1

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