KEGG   ENZYME: 3.4.21.66Help
Entry
EC 3.4.21.66                Enzyme                                 

Name
thermitase;
thermophilic Streptomyces serine proteinase;
Thermoactinomyces vulgaris serine proteinase
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Serine endopeptidases
BRITE hierarchy
Reaction(IUBMB)
Hydrolysis of proteins, including collagen
Comment
A peptidase of family S8 (subtilisin family) from Thermoactinomyces vulgaris containing a single Cys, near the active site His, and inhibited by p-mercuribenzoate. The N-terminal extension of the polypeptide chain relative to subtilisin contributes to Ca2+-binding and the high thermostability. The amino acid composition and properties of the thermostable enzyme from Streptomyces rectus var. proteolyticus (formerly included in EC 3.4.21.14) are closely similar [1,2].
History
EC 3.4.21.66 created 1992
Orthology
K08651  thermitase
Genes
PPHE: PP2015_1892
TIG: THII_2403
KAK: Kalk_10505
AZA: AZKH_4121
AZI: AzCIB_4097
TCL: Tchl_1439
TAK: Tharo_3038
MYM: A176_003310
BBAT: Bdt_2613
BBW: BDW_09700
BBAC: EP01_09630
BEX: A11Q_1794
BAN: BA_2380
BAR: GBAA_2380
BAT: BAS2216
BAI: BAA_2438
BANT: A16_24130
BANR: A16R_24400
BANS: BAPAT_2274
BANV: DJ46_1169(apr)
BCE: BC2316
BCA: BCE_2410
BCQ: BCQ_2306
BCX: BCA_2447
BNC: BCN_2301
BCF: bcf_11840
BCER: BCK_23010
BTL: BALH_2119
BTT: HD73_2601
BTHI: BTK_12915
BTM: MC28_1586
BTI: BTG_08260
BTW: BF38_3565(apr)
BMYC: DJ92_4728
OIH: OB2598
TPED: TPE_1950(prtP)
GBA: J421_1123
SGN: SGRA_1885
IAL: IALB_0145
 » show all
Taxonomy
Reference
1  [PMID:4711613]
  Authors
Mizusawa K, Yoshida F.
  Title
Thermophilic Streptomyces alkaline proteinase. II. The role of a sulfhydryl group and the conformational stability.
  Journal
J Biol Chem 248:4417-23 (1973)
Reference
2  [PMID:4373436]
  Authors
Borgia P, Campbell LL.
  Title
Properties of two homologous alkaline proteases from Streptomyces rectus.
  Journal
J Bacteriol 120:1109-15 (1974)
Reference
3  [PMID:7051706]
  Authors
Kleine R.
  Title
Properties of thermitase, a thermostable serine protease from Thermoactinomyces vulgaris.
  Journal
Acta Biol Med Ger 41:89-102 (1982)
Reference
4
  Authors
Meloun, B., Baudy, M., Kostka, V., Hausdorf, G., Frommel, C. and Hohne, W.E.
  Title
Complete primary structure of thermitase from Thermoactinomyces vulgaris and its structural features related to the subtilisin-type proteinases.
  Journal
FEBS Lett 183:195-200 (1985)
Reference
5  [PMID:2196375]
  Authors
Teplyakov AV, Kuranova IP, Harutyunyan EH, Vainshtein BK, Frommel C, Hohne WE, Wilson KS.
  Title
Crystal structure of thermitase at 1.4 A resolution.
  Journal
J Mol Biol 214:261-79 (1990)
DOI:10.1016/0022-2836(90)90160-N
Other DBs
ExplorEnz - The Enzyme Database: 3.4.21.66
IUBMB Enzyme Nomenclature: 3.4.21.66
ExPASy - ENZYME nomenclature database: 3.4.21.66
BRENDA, the Enzyme Database: 3.4.21.66
CAS: 69772-87-8

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