KEGG   ENZYME: 3.4.21.68Help
Entry
EC 3.4.21.68                Enzyme                                 

Name
t-plasminogen activator;
tissue plasminogen activator;
plasminogen activator, tissue-type;
tissue-type plasminogen activator;
tPA;
t-PA
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Serine endopeptidases
BRITE hierarchy
Reaction(IUBMB)
Specific cleavage of Arg!Val bond in plasminogen to form plasmin
Comment
A peptidase of family S1 (trypsin family) from a wide variety of mammalian tissues, especially endothelial cells. Secreted as a single chain precursor which is cleaved to a two-chain form by plasmin. Activity is considerably enhanced by fibrin. Formerly included in EC 3.4.21.31 and EC 3.4.99.26
History
EC 3.4.21.68 created 1972 as EC 3.4.99.26, transferred 1978 as EC 3.4.21.31, part transferred 1992 to EC 3.4.21.68
Orthology
K01343  tissue plasminogen activator
Genes
HSA: 5327(PLAT)
PTR: 472748(PLAT)
PPS: 100991098(PLAT)
GGO: 101140046(PLAT)
PON: 100173068(PLAT)
NLE: 100586515(PLAT)
MCC: 704475(PLAT)
MCF: 102145462(PLAT)
CSAB: 103215685(PLAT)
RRO: 104668281(PLAT)
RBB: 108540718(PLAT)
CJC: 100391990(PLAT)
SBQ: 101046492(PLAT)
MMU: 18791(Plat)
RNO: 25692(Plat)
CGE: 100750775(Plat)
NGI: 103749408(Plat)
HGL: 101713266(Plat)
CCAN: 109683543(Plat)
OCU: 100009556(PLAT)
TUP: 102468654(PLAT)
CFA: 482840(PLAT)
AML: 100471928(PLAT)
UMR: 103677060(PLAT)
ORO: 101377749(PLAT)
FCA: 101100299(PLAT)
PTG: 102964598(PLAT)
AJU: 106990011(PLAT)
BTA: 281407(PLAT)
BOM: 102281728(PLAT)
BIU: 109553480(PLAT)
PHD: 102332585(PLAT)
CHX: 102187371(PLAT)
OAS: 101118332(PLAT)
SSC: 397121(PLAT)
CFR: 102507711(PLAT)
CDK: 105086430(PLAT)
BACU: 103012605(PLAT)
LVE: 103088899(PLAT)
OOR: 101285324(PLAT)
ECB: 100050239(PLAT)
EPZ: 103562905(PLAT)
EAI: 106828955(PLAT)
MYB: 102261113(PLAT)
MYD: 102775541(PLAT)
HAI: 109386253(PLAT)
RSS: 109452830(PLAT)
PALE: 102893511(PLAT)
LAV: 100657644(PLAT)
TMU: 101350991
MDO: 100033029(PLAT)
SHR: 100928675(PLAT)
OAA: 100078960(PLAT)
GGA: 426791(PLAT)
MGP: 100542402(PLAT)
CJO: 107323741(PLAT)
APLA: 101790558(PLAT)
ACYG: 106044399(PLAT)
TGU: 100227960(PLAT)
GFR: 102034369(PLAT)
FAB: 101819512(PLAT)
PHI: 102108905(PLAT)
PMAJ: 107213913(PLAT)
CCAE: 111938865(PLAT)
CCW: 104694864(PLAT)
FPG: 106112248(PLAT)
FCH: 106631098(PLAT)
CLV: 102093040(PLAT)
EGZ: 104135301(PLAT)
AAM: 106487785(PLAT)
ASN: 102369638(PLAT)
AMJ: 102560959(PLAT)
PSS: 102450593(PLAT)
CMY: 102932303(PLAT)
CPIC: 101945243(PLAT)
ACS: 100568270(plat)
PVT: 110072150(PLAT)
PBI: 103047961(PLAT)
GJA: 107107821(PLAT)
XLA: 399010(plat.L)
XTR: 594962(plat)
NPR: 108791447(PLAT)
DRE: 100320028(plat)
SRX: 107754398
CCAR: 109095149
IPU: 108265556(plat)
AMEX: 103040332(plat)
TRU: 101062205(plat)
LCO: 104921663(plat)
NCC: 104956683(plat)
MZE: 101472111(plat)
OLA: 100049466(plat)
XMA: 102223519(plat)
PRET: 103470022(plat)
NFU: 107394261(plat)
KMR: 108233564(plat)
CSEM: 103397764(plat)
LCF: 108881585(plat)
SDU: 111236537(plat)
HCQ: 109527086(plat)
BPEC: 110153526(plat)
MALB: 109956492(plat)
ELS: 105014297(plat)
SFM: 108920189(plat)
LCM: 102346134(PLAT)
CMK: 103181893(plau)
PMAC: 106708186
BBT: BBta_1433
 » show all
Taxonomy
Reference
1  [PMID:6337343]
  Authors
Pennica D, Holmes WE, Kohr WJ, Harkins RN, Vehar GA, Ward CA, Bennett WF, Yelverton E, Seeburg PH, Heyneker HL, Goeddel DV, Collen D.
  Title
Cloning and expression of human tissue-type plasminogen activator cDNA in E. coli.
  Journal
Nature 301:214-21 (1983)
  Sequence
[hsa:5327]
Reference
2  [PMID:3148826]
  Authors
Loskutoff DJ, Schleef RR.
  Title
Plasminogen activators and their inhibitors.
  Journal
Methods Enzymol 163:293-302 (1988)
Reference
3  [PMID:2962643]
  Authors
Petersen LC, Johannessen M, Foster D, Kumar A, Mulvihill E.
  Title
The effect of polymerised fibrin on the catalytic activities of one-chain tissue-type plasminogen activator as revealed by an analogue resistant to plasmin cleavage.
  Journal
Biochim Biophys Acta 952:245-54 (1988)
Reference
4  [PMID:3148827]
  Authors
Verheijen JH.
  Title
Tissue-type plasminogen activator and fast-acting plasminogen activator inhibitor in plasma.
  Journal
Methods Enzymol 163:302-9 (1988)
Reference
5  [PMID:2496643]
  Authors
Gerard RD, Meidell RS.
  Title
Regulation of tissue plasminogen activator expression.
  Journal
Annu Rev Physiol 51:245-62 (1989)
DOI:10.1146/annurev.ph.51.030189.001333
Reference
6
  Authors
Collen, D., Lijnen, H. R. and Verstraete, M.
  Title
The fibrinolytic system and its disorders.
  Journal
In: Handin, R.I., Lux, S.E. and Stossel, J.P. (Eds.), Blood: Principles and Practice of Hematology, 2nd ed., J.B.Lippincott Company, Philadelphia, 1990.
Other DBs
ExplorEnz - The Enzyme Database: 3.4.21.68
IUBMB Enzyme Nomenclature: 3.4.21.68
ExPASy - ENZYME nomenclature database: 3.4.21.68
BRENDA, the Enzyme Database: 3.4.21.68
CAS: 139639-23-9

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