EC                Enzyme                                 

yama protein
Acting on peptide bonds (peptidases);
Cysteine endopeptidases
Strict requirement for an Asp residue at positions P1 and P4. It has a preferred cleavage sequence of Asp-Xaa-Xaa-Asp! with a hydrophobic amino-acid residue at P2 and a hydrophilic amino-acid residue at P3, although Val or Ala are also accepted at this position
Caspase-3 is an effector/executioner caspase, as are caspase-6 (EC and caspase-7 (EC [5]. These caspases are responsible for the proteolysis of the majority of cellular polypeptides [e.g. poly(ADP-ribose) polymerase (PARP)], which leads to the apoptotic phenotype [3,5]. Procaspase-3 can be activated by caspase-1 (EC, caspase-8 (EC, caspase-9 (EC and caspase-10 (EC as well as by the serine protease granzyme B [1]. Caspase-3 can activate procaspase-2 (EC [2]. Activation occurs by inter-domain cleavage followed by removal of the N-terminal prodomain [6]. Although Asp-Glu-(Val/Ile)-Asp is thought to be the preferred cleavage sequence, the enzyme can accommodate different residues at P2 and P3 of the substrate [4]. Like caspase-2, a hydrophobic residue at P5 of caspase-3 leads to more efficient hydrolysis, e.g. (Val/Leu)-Asp-Val-Ala-Asp! is a better substrate than Asp-Val-Ala-Asp! . This is not the case for caspase-7 [4]. Belongs in peptidase family C14.
EC created 2007
K02187  caspase 3
HSA: 836(CASP3)
PTR: 461669(CASP3)
PPS: 100974934(CASP3)
GGO: 101126742(CASP3)
PON: 100435491(CASP3)
NLE: 100585064(CASP3)
MCC: 694473(CASP3)
MCF: 102144182(CASP3)
CSAB: 103236614(CASP3)
CATY: 105593734 105597738(CASP3)
PANU: 101023728(CASP3)
RRO: 104666783 104673791(CASP3)
RBB: 108512689
PTEH: 111548479(CASP3) 111551382
CJC: 100387570(CASP3)
SBQ: 101029697(CASP3)
MMUR: 105880624 105882502(CASP3)
MMU: 12367(Casp3)
MCAL: 110299851(Casp3)
MPAH: 110336608(Casp3)
RNO: 25402(Casp3)
MCOC: 116069240(Casp3)
CGE: 100689061(Casp3)
PLEU: 114693817(Casp3)
NGI: 103744668 103750475(Casp3)
CPOC: 100718309(Casp3)
OCU: 100008840(CASP3)
OPI: 101531640(CASP3)
TUP: 102472926(CASP3) 102487119
CFA: 403567(CASP3)
VVP: 112934766(CASP3)
VLG: 121489913(CASP3)
AML: 100469056(CASP3)
UMR: 103680991(CASP3)
UAH: 113262259(CASP3)
ORO: 101371336(CASP3)
ELK: 111144607
MPUF: 101690858(CASP3)
EJU: 114206181(CASP3)
MLX: 118010074(CASP3)
FCA: 493932(CASP3)
PYU: 121039672(CASP3)
PBG: 122470647(CASP3)
PTG: 102954729(CASP3)
PPAD: 109251018(CASP3)
AJU: 106979326(CASP3)
HHV: 120246375(CASP3)
BTA: 408016(CASP3)
BOM: 102287348(CASP3)
BIU: 109553316(CASP3)
BBUB: 102415768(CASP3)
CHX: 102177031(CASP3)
OAS: 114116869 443031(CASP3)
ODA: 120865721(CASP3)
CCAD: 122432443(CASP3)
SSC: 397244(CASP3)
CFR: 102520133(CASP3)
CBAI: 105067685(CASP3)
CDK: 105084433(CASP3)
BACU: 103019939(CASP3)
LVE: 103073819(CASP3)
OOR: 101289847(CASP3)
DLE: 111173401(CASP3)
PCAD: 102975528 102977692(CASP3)
PSIU: 116746660(CASP3)
ECB: 100034083(CASP3)
EPZ: 103549132(CASP3)
EAI: 106830081(CASP3)
MYB: 102258599(CASP3)
MYD: 102760961(CASP3)
MMYO: 118650210(CASP3)
MLF: 102438419(CASP3)
MNA: 107535740(CASP3)
PKL: 118729116(CASP3)
HAI: 109395706(CASP3)
DRO: 112306749(CASP3)
SHON: 118993073(CASP3)
AJM: 119036917(CASP3)
PDIC: 114508735(CASP3)
MMF: 118622705(CASP3)
RFQ: 117021507(CASP3)
PALE: 102897289(CASP3)
PGIG: 120618435(CASP3)
PVP: 105294710(CASP3)
RAY: 107510456(CASP3)
MJV: 108389793(CASP3)
TOD: 119257947(CASP3)
LAV: 100672256(CASP3)
TMU: 101354307
MDO: 654273(CASP3) 654275
SHR: 100922500(CASP3) 100922757
PCW: 110222594(CASP3)
OAA: 100087105(CASP3)
GGA: 395476(CASP3)
PCOC: 116238124(CASP3)
MGP: 100548182(CASP3)
CJO: 107313280(CASP3)
NMEL: 110397291(CASP3)
APLA: 101805296(CASP3)
ACYG: 106035172(CASP3)
TGU: 100230799(CASP3)
LSR: 110477516(CASP3)
SCAN: 103825669(CASP3)
PMOA: 120497893(CASP3)
OTC: 121348441(CASP3)
PRUF: 121360209(CASP3)
GFR: 102035830(CASP3)
FAB: 101808212(CASP3)
PHI: 102104322(CASP3)
PMAJ: 107203069(CASP3)
CCAE: 111945572(CASP3)
CCW: 104690575(CASP3)
ETL: 114055579(CASP3)
FPG: 101913355(CASP3) 101922533
FCH: 102050683 102056397(CASP3)
CLV: 102089427(CASP3)
EGZ: 104129473(CASP3)
NNI: 104021286(CASP3)
ACUN: 113479376(CASP3)
TALA: 104365328(CASP3)
PADL: 103922530(CASP3) 103926227
AAM: 106484082(CASP3)
AROW: 112974750(CASP3)
NPD: 112942609(CASP3)
DNE: 112986139(CASP3)
ASN: 102369935 102383553(CASP3)
AMJ: 102572621(CASP3) 102576234(CASP14)
CPOO: 109309794(CASP3)
GGN: 109301036(CASP3)
PSS: 102463409
CMY: 102942062(CASP3)
CPIC: 101948246(CASP3)
TST: 117877835(CASP3)
CABI: 116836650(CASP3)
MRV: 120406161(CASP3)
ACS: 100563931(casp3)
PVT: 110083865(CASP3)
SUND: 121930895(CASP3)
PBI: 103049711(CASP3)
PMUR: 107302670(CASP3)
TSR: 106539846(CASP3)
PGUT: 117654566(CASP3)
VKO: 123033290(CASP3)
PMUA: 114585959 114604250(CASP3)
ZVI: 118083396(CASP3)
GJA: 107115654(CASP3)
XLA: 100036910(casp3.2.L) 397720(casp3.2.S)
XTR: 100151728(casp3.2) 100486222(casp3.1)
DRE: 140621(casp3a) 566604(casp3b)
IPU: 100528811(casp3) 108260860
EEE: 113581517(casp3) 113586537
TRU: 446083(casp3)
LCO: 104928227 104936945(caspase-3)
NCC: 104959742 104967108(casp3)
CGOB: 115010976(casp3) 115014922
ELY: 117254954(casp3a) 117261186(casp3b)
PLEP: 121942065(casp3a) 121948074(casp3b) 121963193
ECRA: 117951380 117960699(casp3a)
PPUG: 119211291(casp3b) 119217562(casp3a)
MSAM: 119888653 119898808(casp3a)
CUD: 121506610 121508374(casp3a)
OLA: 100049215(casp3) 100049265
OML: 112137236(casp3a) 112153020
XCO: 114139499 114144355(casp3)
GAF: 122830167(casp3a) 122837161
CVG: 107090480 107094160(casp3)
CTUL: 119774386(casp3a) 119794097
NFU: 107390752(casp3) 107397381
KMR: 108234461(casp3a) 108240012
CSEM: 103383467 103391460(casp3)
SSEN: 122770713(casp3a) 122778867(casp3b)
XGL: 120785392 120800586(casp3a)
MALB: 109957512(casp3) 109972113
SASA: 100194892(casp3) 106602722(CASP3) 106612018
OTW: 112231666(casp3a) 112248666 112255992(casp3b) 112261439
OMY: 100653443(casp3b) 110497496 110525880 110534285(casp3a)
SNH: 120035095 120040359 120052764(casp3a) 120062476(casp3b)
ELS: 105006735(casp3) 105029385(casp3)
SFM: 108924572(casp3) 114912449
PKI: 111853593(casp3)
AANG: 118228056(casp3a) 118231090
LOC: 102691196
LCM: 106705928(CASP3)
APLC: 110975794
DPE: 113565090
DMN: 108158700
DHE: 111592184
MDE: 101899570
LCQ: 111676405
AARA: 120900924
AAG: 5567300
TPRE: 106649650
PPYR: 116180848
PXU: 106127530
FOC: 113216828
DMK: 116936557
PVM: 113829172
HAZT: 108673778
VDE: 111249074
VJA: 111272320
MYI: 110452312
OBI: 106868738
LAK: 106173180
EGL: EGR_04745
NVE: 5502486
EPA: 110247107
 » show all
1  [PMID:11123933]
Krebs JF, Srinivasan A, Wong AM, Tomaselli KJ, Fritz LC, Wu JC.
Heavy membrane-associated caspase 3: identification, isolation, and characterization.
Biochemistry 39:16056-63 (2000)
2  [PMID:9261102]
Li H, Bergeron L, Cryns V, Pasternack MS, Zhu H, Shi L, Greenberg A, Yuan J.
Activation of caspase-2 in apoptosis.
J Biol Chem 272:21010-7 (1997)
Nicholson, D. and Thornberry, N.A.
Caspase-3 and caspase-7.
In: Barrett, A.J., Rawlings, N.D. and Woessner, J.F. (Eds.), Handbook of Proteolytic Enzymes, 2nd ed., Elsevier, London, 2004, p. 1298-1302.
4  [PMID:16781734]
Fang B, Boross PI, Tozser J, Weber IT.
Structural and kinetic analysis of caspase-3 reveals role for s5 binding site in substrate recognition.
J Mol Biol 360:654-66 (2006)
5  [PMID:11104820]
Chang HY, Yang X.
Proteases for cell suicide: functions and regulation of caspases.
Microbiol Mol Biol Rev 64:821-46 (2000)
6  [PMID:8665848]
Martin SJ, Amarante-Mendes GP, Shi L, Chuang TH, Casiano CA, O'Brien GA, Fitzgerald P, Tan EM, Bokoch GM, Greenberg AH, Green DR.
The cytotoxic cell protease granzyme B initiates apoptosis in a cell-free system by proteolytic processing and activation of the ICE/CED-3 family protease, CPP32, via a novel two-step mechanism.
EMBO J 15:2407-16 (1996)
Other DBs
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IUBMB Enzyme Nomenclature:
ExPASy - ENZYME nomenclature database:
BRENDA, the Enzyme Database:
CAS: 169592-56-7

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