KEGG   ENZYME: 3.4.23.32Help
Entry
EC 3.4.23.32                Enzyme                                 

Name
scytalidopepsin B;
Scytalidium aspartic proteinase B;
Ganoderma lucidum carboxyl proteinase;
Ganoderma lucidum aspartic proteinase;
Scytalidium lignicolum aspartic proteinase B;
SLB
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Aspartic endopeptidases
BRITE hierarchy
Reaction(IUBMB)
Hydrolysis of proteins with broad specificity, cleaving Phe24!Phe, but not Leu15-Tyr and Phe25-Tyr in the B chain of insulin
Comment
A second endopeptidase from Scytalidium lignicolum (see scytalidopepsin A) that is insensitive to pepstatin and methyl 2-diazoacetamidohexanoate. 1,2-Epoxy-3-(p-nitrophenoxy)propane reacts with Glu53, which replaces one of the aspartic residues at the active centre. One of the smallest aspartic endopeptidases active as the monomer, with Mr 22,000. Similarly inhibitor-resistant endopeptidases are found in the basidiomycetes Lentinus edodes [1] and Ganoderma lucidum [3], and in Polyporus tulipiferae [4], a second endopeptidase distinct from polyporopepsin, but these are of typical aspartic endopeptidase size, Mr about 36,000. Type example of peptidase family G1.
History
EC 3.4.23.32 created 1992
Orthology
K20743  scytalidopepsin B
Reference
1
  Authors
Terashita, T., Oda, K., Kono, M. and Murao, S.
  Title
Streptomyces pepsin inhibitor-insensitive carboxyl proteinase from Lentinus edodes.
  Journal
Agric Biol Chem 45:1937-1943 (1981)
Reference
2  [PMID:6370989]
  Authors
Maita T, Nagata S, Matsuda G, Maruta S, Oda K, Murao S, Tsuru D.
  Title
Complete amino acid sequence of Scytalidium lignicolum acid protease B.
  Journal
J Biochem (Tokyo) 95:465-75 (1984)
  Sequence
Reference
3
  Authors
Terashita, T., Oda, K., Kono, M. and Murao, S.
  Title
Streptomyces pepsin inhibitor-insensitive carboxyl proteinase from Ganoderma lucidum.
  Journal
Agric Biol Chem 48:1029-1035 (1984)
Reference
4
  Authors
Kobayashi, H., Kusakabe, I. and Murakami, K.
  Title
Purification and characterization of a pepstatin-insensitive carboxyl proteinase from Polyporus tulipiferae (Irpex lacteus).
  Journal
Agric Biol Chem 49:2393-2397 (1985)
Reference
5  [PMID:3519605]
  Authors
Tsuru D, Shimada S, Maruta S, Yoshimoto T, Oda K, Murao S, Miyata T, Iwanaga S.
  Title
Isolation and amino acid sequence of a peptide containing an epoxide-reactive residue from the thermolysin-digest of Scytalidium lignicolum acid protease B.
  Journal
J Biochem (Tokyo) 99:1537-9 (1986)
Other DBs
ExplorEnz - The Enzyme Database: 3.4.23.32
IUBMB Enzyme Nomenclature: 3.4.23.32
ExPASy - ENZYME nomenclature database: 3.4.23.32
BRENDA, the Enzyme Database: 3.4.23.32
CAS: 104781-89-7

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