KEGG   ENZYME: 3.4.24.27Help
Entry
EC 3.4.24.27                Enzyme                                 

Name
thermolysin;
Bacillus thermoproteolyticus neutral proteinase;
thermoase;
thermoase Y10;
TLN
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Metalloendopeptidases
BRITE hierarchy
Reaction(IUBMB)
Preferential cleavage: !Leu > !Phe
Comment
A thermostable extracellular metalloendopeptidase containing four calcium ions. Enzymes that may be species variants of thermolysin are reported from Micrococcus caseolyticus [4] and Aspergillus oryzae [5]. Type example of peptidase family M4. Closely related but distinct enzymes are aeromonolysin, pseudolysin, bacillolysin, aureolysin and mycolysin
History
EC 3.4.24.27 created 1972 as EC 3.4.24.4, part transferred 1992 to EC 3.4.24.27
Orthology
K08603  thermolysin
Genes
HAU: Haur_3645
Taxonomy
Reference
1  [PMID:5954368]
  Authors
Ohta Y, Ogura Y, Wada A.
  Title
Thermostable protease from thermophilic bacteria. I. Thermostability, physiocochemical properties, and amino acid composition.
  Journal
J Biol Chem 241:5919-25 (1966)
Reference
2  [PMID:4967801]
  Authors
Morihara K, Tsuzuki H, Oka T.
  Title
Comparison of the specificities of various neutral proteinases from microorganisms.
  Journal
Arch Biochem Biophys 123:572-88 (1968)
DOI:10.1016/0003-9861(68)90179-3
Reference
3  [PMID:5823940]
  Authors
Latt SA, Holmquist B, Vallee BL.
  Title
Thermolysin: a zinc metalloenzyme.
  Journal
Biochem Biophys Res Commun 37:333-9 (1969)
DOI:10.1016/0006-291X(69)90739-6
Reference
4  [PMID:5551628]
  Authors
Desmazeaud MJ, Hermier JH.
  Title
[Specificity of the nuclear protease produced by Micrococcus caseolyticus]
  Journal
Eur J Biochem 19:51-5 (1971)
DOI:10.1111/j.1432-1033.1971.tb01286.x
Reference
5  [PMID:5004124]
  Authors
Morihara K, Tsuzuki H.
  Title
Comparative study of various neutral proteinases from microorganisms: specificity with oligopeptides.
  Journal
Arch Biochem Biophys 146:291-6 (1971)
DOI:10.1016/S0003-9861(71)80066-8
Reference
6  [PMID:18663848]
  Authors
Titani K, Hermodson MA, Ericsson LH, Walsh KA, Neurath H
  Title
Amino-acid sequence of thermolysin.
  Journal
Nat New Biol 238:35-7 (1972)
  Sequence
Reference
7
  Authors
Matthews, B.
  Title
W. Structural basis of the action of thermolysin and related zinc peptidases.
  Journal
Acc Chem Res 21:333-340 (1988)
Other DBs
ExplorEnz - The Enzyme Database: 3.4.24.27
IUBMB Enzyme Nomenclature: 3.4.24.27
ExPASy - ENZYME nomenclature database: 3.4.24.27
BRENDA, the Enzyme Database: 3.4.24.27
CAS: 9073-78-3

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