KEGG   ENZYME: 3.4.24.39Help
Entry
EC 3.4.24.39                Enzyme                                 

Name
deuterolysin;
Penicillium roqueforti protease II;
microbial neutral proteinase II;
acid metalloproteinase;
neutral proteinase II;
Penicillium roqueforti metalloproteinase
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Metalloendopeptidases
BRITE hierarchy
Reaction(IUBMB)
Preferential cleavage of bonds with hydrophobic residues in P1'; also Asn3!Gln and Gly8!Ser bonds in insulin B chain
Comment
Proteolytic activity found in Penicillium roqueforti [4], P. caseicolum [4], Aspergillus sojae [3] and A. oryzae [1,5]. Optimum pH of 5 for digesting various proteins. Strong action on protamine and histones. Insensitive to phosphoramidon. About 20 kDa. A distinct Aspergillus sojae endopeptidase is larger and has a neutral pH optimum. Type example of peptidase family M35. Formerly included in EC 3.4.24.4
History
EC 3.4.24.39 created 1972 as EC 3.4.24.4, part transferred 1992 to EC 3.4.24.39
Orthology
K19305  deuterolysin
Genes
NCR: NCU05071
NTE: NEUTE1DRAFT113287(NEUTE1DRAFT_113287)
SMP: SMAC_07573
MGR: MGG_03029 MGG_03808 MGG_07038 MGG_10927 MGG_15370 MGG_16546 MGG_17758
TMN: UCRPA7_5057
FGR: FGSG_08289
FPU: FPSE_09518
FVR: FVEG_01804 FVEG_12487
FOX: FOXG_02952 FOXG_17405
NHE: NECHADRAFT_26618 NECHADRAFT_36693 NECHADRAFT_87000
PTE: PTT_00390
ABP: AGABI1DRAFT113068(AGABI1DRAFT_113068)
ABV: AGABI2DRAFT188701(AGABI2DRAFT_188701)
 » show all
Taxonomy
Reference
1
  Authors
Nakadai, T., Nasuno, S. and Iguchi, N.
  Title
Purification and properties of neutral proteinase II from Aspergillus oryzae.
  Journal
Agric Biol Chem 37:2703-2708 (1973)
Reference
2  [PMID:4219726]
  Authors
Gripon JC, Hermier J.
  Title
[The proteolytic system of Penicillium roqueforti. III. - Purification, properties and specificity of a protease inhibited by E.D.T.A]
  Journal
Biochimie 56:1323-32 (1974)
Reference
3
  Authors
Sekine, H.
  Title
, Neutral proteinases I and II of Aspergillus sojae action on various substrates.
  Journal
Agric Biol Chem 40:703-709 (1976)
Reference
4  [PMID:6998789]
  Authors
Gripon JC, Auberger B, Lenoir J.
  Title
Metalloproteases from Penicillium caseicolum and P. roqueforti: comparison of specificity and chemical characterization.
  Journal
Int J Biochem 12:451-5 (1980)
Reference
5
  Authors
Vaganova, T.I., Ivanova, N.M. and Stepanov, V.M.
  Title
Isolation and properties of the "acid" metalloproteinase from Aspergillus oryzae.
  Journal
Biochemistry (Mosc) 53:1171-1178 (1988)
Other DBs
ExplorEnz - The Enzyme Database: 3.4.24.39
IUBMB Enzyme Nomenclature: 3.4.24.39
ExPASy - ENZYME nomenclature database: 3.4.24.39
BRENDA, the Enzyme Database: 3.4.24.39
CAS: 247028-11-1

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