KEGG   ENZYME: 3.4.24.40Help
Entry
EC 3.4.24.40                Enzyme                                 

Name
serralysin;
Pseudomonas aeruginosa alkaline proteinase;
Escherichia freundii proteinase;
Serratia marcescens extracellular proteinase;
Serratia marcescens metalloproteinase;
Pseudomonas aeruginosa alk. protease;
Serratia marcescens metalloprotease
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Metalloendopeptidases
BRITE hierarchy
Reaction(IUBMB)
Preferential cleavage of bonds with hydrophobic residues in P1'
Comment
A 50 kDa extracellular endopeptidase from Pseudomonas aeruginosa [1,2,6], Escherichia freundii [3], Serratia marcescens [4,5,6] and Erwinia chrysanthemi [7]. There is broad specificity in cleavage of the insulin B chain, with some species variations. The pH optimum for digesting various proteins is about 9 - 10. In peptidase family M10 (interstitial collagenase family). Formerly included in EC 3.4.24.4
History
EC 3.4.24.40 created 1972 as EC 3.4.24.4, part transferred 1992 to EC 3.4.24.40
Orthology
K01406  serralysin
Genes
HDE: HDEF_0400
YPE: YPO3973
YPK: y3857
YPH: YPC_4478
YPA: YPA_3800
YPN: YPN_3621
YPM: YP_3335
YPP: YPDSF_3337
YPG: YpAngola_A4088
YPZ: YPZ3_2192
YPD: YPD4_3494
YPX: YPD8_3497
YPW: CH59_1921(prtA)
YPJ: CH55_2652(prtA)
YPV: BZ15_3732(prtA)
YPL: CH46_1084(prtA)
YPS: YPTB3814
YPO: BZ17_2771(prtA)
YPY: YPK_0118
YPB: YPTS_4028
YPQ: DJ40_2581(prtA)
YPU: BZ21_3166(prtA)
YPR: BZ20_2278(prtA)
YPC: BZ23_3440(prtA)
YPF: BZ19_3302(prtA)
YEN: YE4052
YEY: Y11_31431
YEW: CH47_3679(prtA)
YET: CH48_1504(papra)
YAL: AT01_2628(prtA) AT01_2629(aprA)
YFR: AW19_3402(prtA)
YIN: CH53_1609(prtA) CH53_3001(prtA)
YKR: CH54_2388
YRO: CH64_2458(prtG)
YRU: BD65_101(prtA)
SPLY: Q5A_000940
SMAF: D781_0184
ECA: ECA2785(prtW)
PATR: EV46_13620
PATO: GZ59_17740(prtW)
PCT: PC1_1553
PEC: W5S_2894
DDD: Dda3937_03860(prtC) Dda3937_03861(prtA)
DDQ: DDI_1978
EAM: EAMY_3581(prtA)
EAY: EAM_3368(prtA)
PLU: plu0655(prtA)
PAY: PAU_00605
PMR: PMI0279(zapA)
PMIB: BB2000_0430(zapA)
XBO: XBJ1_0491
XBV: XBW1_4194(prtA)
XNE: XNC1_4025
XNM: XNC2_3878(prtA)
XDO: XDD1_3329(prtA)
XPO: XPG1_2747(prtA)
MMK: MU9_1121
HPAS: JL26_08850
HPAK: JT17_07530
BTRA: F544_22240
PAE: PA1249(aprA)
PAEV: N297_1288(aprA)
PAEI: N296_1288(aprA)
PAU: PA14_48060(aprA)
PAP: PSPA7_4143(aprA)
PAG: PLES_40631(aprA)
PAF: PAM18_3782(aprA)
PNC: NCGM2_2131(aprA)
PAEB: NCGM1900_1327(aprA)
PAEP: PA1S_19670
PAEM: U769_19510
PAEL: T223_20760
PAEU: BN889_01328(aprA)
PAEG: AI22_14295
PAEC: M802_1285(aprA)
PAEO: M801_1288(aprA)
PMY: Pmen_4542
PSB: Psyr_3163
PSYR: N018_15885
PFL: PFL_2483(aprX) PFL_3210(aprA)
PPRC: PFLCHA0_c25470(aprA1) PFLCHA0_c32400(aprA2)
PPRO: PPC_2525 PPC_3236(aprA)
PFS: PFLU_3146
PFE: PSF113_2949(aprA)
PFC: PflA506_2617(aprA)
PFW: PF1751_v1c26180(aprA)
PFB: VO64_0239
PMAN: OU5_0345
PEN: PSEEN1550(aprA)
PSEC: CCOS191_3824(aprA)
PANR: A7J50_2785
PSIL: PMA3_13735
ACB: A1S_1073
ABAD: ABD1_24060
ABAZ: P795_4860
AZA: AZKH_1421
MES: Meso_0616
SME: SMa0034
SMER: DU99_25360
SMD: Smed_5287
SFD: USDA257_c14380(prtC) USDA257_c28100(zapA)
SAME: SAMCFNEI73_pC1314(prtC)
ARA: Arad_4711
RLE: RL0790
LAA: WSI_01255
LSO: CKC_02265
BJA: bll6027
BRS: S23_22440
VGO: GJW-30_1_00084(prtC)
AZC: AZC_2666
MEX: Mext_3220
MDI: METDI4008
MCH: Mchl_3544
MET: M446_6774
MNO: Mnod_0800
PHL: KKY_2563
HDI: HDIA_4818
CCR: CC_0746
CCS: CCNA_00783(sapA)
CAK: Caul_4884
CSE: Cseg_3618
PZU: PHZ_c3294
RSP: RSP_0230
JAN: Jann_1557
PDE: Pden_0822
DSH: Dshi_3402
PSF: PSE_0869
PGD: Gal_04388
SWI: Swit_0627
SPHD: HY78_23530
ADO: A6F68_02706(prtC)
RCE: RC1_3143
SYW: SYNW2304
SYG: sync_1260
PRM: EW15_0487
CYT: cce_0913
TER: Tery_0100
AVA: Ava_3643
 » show all
Taxonomy
Reference
1  [PMID:4967801]
  Authors
Morihara K, Tsuzuki H, Oka T.
  Title
Comparison of the specificities of various neutral proteinases from microorganisms.
  Journal
Arch Biochem Biophys 123:572-88 (1968)
DOI:10.1016/0003-9861(68)90179-3
Reference
2  [PMID:4199986]
  Authors
Morihara K, Tsuzuki H, Oka T.
  Title
On the specificity of Pseudomonas aeruginosa alkaline proteinase with synthetic peptides.
  Journal
Biochim Biophys Acta 309:414-29 (1973)
DOI:10.1016/0005-2744(73)90040-5
  Sequence
[pae:PA1249]
Reference
3  [PMID:4212288]
  Authors
Nakajima M, Mizusawa K, Yoshida F.
  Title
Purification and properties of an extracellular proteinase of psychrophilic Escherichia freundii.
  Journal
Eur J Biochem 44:87-96 (1974)
DOI:10.1111/j.1432-1033.1974.tb03460.x
Reference
4  [PMID:383155]
  Authors
Decedue CJ, Broussard EA 2nd, Larson AD, Braymer HD.
  Title
Purification and characterization of the extracellular proteinase of Serratia marcescens.
  Journal
Biochim Biophys Acta 569:293-301 (1979)
DOI:10.1016/0005-2744(79)90065-2
Reference
5  [PMID:6380155]
  Authors
Doerr M, Traub WH.
  Title
Purification and characterization of two Serratia marcescens proteases.
  Journal
Zentralbl Bakteriol Mikrobiol Hyg [A] 257:6-19 (1984)
Reference
6  [PMID:3016665]
  Authors
Nakahama K, Yoshimura K, Marumoto R, Kikuchi M, Lee IS, Hase T, Matsubara H.
  Title
Cloning and sequencing of Serratia protease gene.
  Journal
Nucleic Acids Res 14:5843-55 (1986)
DOI:10.1093/nar/14.14.5843
  Sequence
Reference
7  [PMID:2211513]
  Authors
Dahler GS, Barras F, Keen NT.
  Title
Cloning of genes encoding extracellular metalloproteases from Erwinia chrysanthemi EC16.
  Journal
J Bacteriol 172:5803-15 (1990)
DOI:10.1128/JB.172.10.5803-5815.1990
  Sequence
Reference
8  [PMID:2123832]
  Authors
Okuda K, Morihara K, Atsumi Y, Takeuchi H, Kawamoto S, Kawasaki H, Suzuki K, Fukushima J.
  Title
Complete nucleotide sequence of the structural gene for alkaline proteinase from Pseudomonas aeruginosa IFO 3455.
  Journal
Infect Immun 58:4083-8 (1990)
  Sequence
[pae:PA1249]
Other DBs
ExplorEnz - The Enzyme Database: 3.4.24.40
IUBMB Enzyme Nomenclature: 3.4.24.40
ExPASy - ENZYME nomenclature database: 3.4.24.40
BRENDA, the Enzyme Database: 3.4.24.40
CAS: 70851-98-8

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