KEGG   ENZYME: 3.4.24.42Help
Entry
EC 3.4.24.42                Enzyme                                 

Name
atrolysin C;
Crotalus atrox metalloendopeptidase c;
hemorrhagic toxin c and d
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Metalloendopeptidases
BRITE hierarchy
Reaction(IUBMB)
Cleavage of His5!Leu, His10!Leu, Ala14!Leu, Tyr16!Leu and Gly23!Phe bonds in B chain of insulin. With small molecule substrates prefers hydrophobic residue at P2' and small residue such as Ala, Gly at P1
Comment
A 24 kDa hemorrhagic endopeptidase from the venom of the western diamondback rattlesnake (Crotalus atrox) that digests type IV collagen, and exists as two forms, c and d. Phosphoramidon inhibits in the 0.1 mM range. In peptidase family M12 (astacin family). Hemorrhagic toxin-2 of C. ruber ruber has the same Mr and specificity and is a homologue [4,6].
History
EC 3.4.24.42 created 1992
Orthology
K20746  atrolysin C
Reference
1  [PMID:210790]
  Authors
Bjarnason JB, Tu AT.
  Title
Hemorrhagic toxins from Western diamondback rattlesnake (Crotalus atrox) venom: isolation and characterization of five toxins and the role of zinc in hemorrhagic toxin e.
  Journal
Biochemistry 17:3395-404 (1978)
Reference
2  [PMID:3514216]
  Authors
Fox JW, Campbell R, Beggerly L, Bjarnason JB.
  Title
Substrate specificities and inhibition of two hemorrhagic zinc proteases Ht-c and Ht-d from Crotalus atrox venom.
  Journal
Eur J Biochem 156:65-72 (1986)
DOI:10.1111/j.1432-1033.1986.tb09549.x
Reference
3  [PMID:3101740]
  Authors
Bjarnason JB, Fox JW.
  Title
Characterization of two hemorrhagic zinc proteinases, toxin c and toxin d, from western diamondback rattlesnake (Crotalus atrox) venom.
  Journal
Biochim Biophys Acta 911:356-63 (1987)
DOI:10.1016/0167-4838(87)90077-X
Reference
4  [PMID:2949699]
  Authors
Mori N, Nikai T, Sugihara H, Tu AT.
  Title
Biochemical characterization of hemorrhagic toxins with fibrinogenase activity isolated from Crotalus ruber ruber venom.
  Journal
Arch Biochem Biophys 253:108-21 (1987)
DOI:10.1016/0003-9861(87)90643-6
Reference
5  [PMID:2745407]
  Authors
Shannon JD, Baramova EN, Bjarnason JB, Fox JW.
  Title
Amino acid sequence of a Crotalus atrox venom metalloproteinase which cleaves type IV collagen and gelatin.
  Journal
J Biol Chem 264:11575-83 (1989)
  Sequence
Reference
6  [PMID:2081731]
  Authors
Takeya H, Onikura A, Nikai T, Sugihara H, Iwanaga S.
  Title
Primary structure of a hemorrhagic metalloproteinase, HT-2, isolated from the venom of Crotalus ruber ruber.
  Journal
J Biochem (Tokyo) 108:711-9 (1990)
Other DBs
ExplorEnz - The Enzyme Database: 3.4.24.42
IUBMB Enzyme Nomenclature: 3.4.24.42
ExPASy - ENZYME nomenclature database: 3.4.24.42
BRENDA, the Enzyme Database: 3.4.24.42
CAS: 158886-17-0

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