Hydrolases;
Acting on peptide bonds (peptidases);
Metalloendopeptidases
BRITE hierarchy

Cleavage of His5!Leu, His10!Leu, Ala14!Leu, Tyr16!Leu and Gly23!Phe bonds in B chain of insulin. With small molecule substrates prefers hydrophobic residue at P2' and small residue such as Ala, Gly at P1

A 24 kDa hemorrhagic endopeptidase from the venom of the western diamondback rattlesnake (Crotalus atrox) that digests type IV collagen, and exists as two forms, c and d. Phosphoramidon inhibits in the 0.1 mM range. In peptidase family M12 (astacin family). Hemorrhagic toxin-2 of C. ruber ruber has the same Mr and specificity and is a homologue [4,6].

EC 3.4.24.42 created 1992

1  [PMID:210790]

Bjarnason JB, Tu AT.

Hemorrhagic toxins from Western diamondback rattlesnake (Crotalus atrox) venom: isolation and characterization of five toxins and the role of zinc in hemorrhagic toxin e.

Biochemistry 17:3395-404 (1978)
DOI:10.1021/bi00609a033

2  [PMID:3514216]

Fox JW, Campbell R, Beggerly L, Bjarnason JB.

Substrate specificities and inhibition of two hemorrhagic zinc proteases Ht-c and Ht-d from Crotalus atrox venom.

Eur J Biochem 156:65-72 (1986)
DOI:10.1111/j.1432-1033.1986.tb09549.x

3  [PMID:3101740]

Bjarnason JB, Fox JW.

Characterization of two hemorrhagic zinc proteinases, toxin c and toxin d, from western diamondback rattlesnake (Crotalus atrox) venom.

Biochim Biophys Acta 911:356-63 (1987)
DOI:10.1016/0167-4838(87)90077-X

4  [PMID:2949699]

Mori N, Nikai T, Sugihara H, Tu AT.

Biochemical characterization of hemorrhagic toxins with fibrinogenase activity isolated from Crotalus ruber ruber venom.

Arch Biochem Biophys 253:108-21 (1987)
DOI:10.1016/0003-9861(87)90643-6

5  [PMID:2745407]

Shannon JD, Baramova EN, Bjarnason JB, Fox JW

Amino acid sequence of a Crotalus atrox venom metalloproteinase which cleaves type IV collagen and gelatin.

J Biol Chem 264:11575-83 (1989)

[ag:AAA03352]

6  [PMID:2081731]

Takeya H, Onikura A, Nikai T, Sugihara H, Iwanaga S.

Primary structure of a hemorrhagic metalloproteinase, HT-2, isolated from the venom of Crotalus ruber ruber.

J Biochem (Tokyo) 108:711-9 (1990)
DOI:10.1093/oxfordjournals.jbchem.a123270