KEGG   ENZYME: 3.5.1.54Help
Entry
EC 3.5.1.54                 Enzyme                                 

Name
allophanate hydrolase;
allophanate lyase;
AtzF;
TrzF
Class
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In linear amides
BRITE hierarchy
Sysname
urea-1-carboxylate amidohydrolase
Reaction(IUBMB)
urea-1-carboxylate + H2O = 2 CO2 + 2 NH3 [RN:R00005]
Reaction(KEGG)
Substrate
urea-1-carboxylate [CPD:C01010];
H2O [CPD:C00001]
Product
CO2 [CPD:C00011];
NH3 [CPD:C00014]
Comment
Along with EC 3.5.2.15 (cyanuric acid amidohydrolase) and EC 3.5.1.84 (biuret amidohydrolase), this enzyme forms part of the cyanuric-acid metabolism pathway, which degrades s-triazide herbicides, such as atrazine [2-chloro-4-(ethylamino)-6-(isopropylamino)-1,3,5-triazine], in bacteria. The yeast enzyme (but not that from green algae) also catalyses the reaction of EC 6.3.4.6, urea carboxylase, thus bringing about the hydrolysis of urea to CO2 and NH3 in the presence of ATP and bicarbonate. The enzyme from Pseudomonas sp. strain ADP has a narrow substrate specificity, being unable to use the structurally analogous compounds urea, hydroxyurea or methylcarbamate as substrate [6].
History
EC 3.5.1.54 created 1986, modified 2008
Pathway
ec00220  Arginine biosynthesis
ec00791  Atrazine degradation
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K01457  allophanate hydrolase
K14541  urea carboxylase / allophanate hydrolase
Genes
SCE: YBR208C(DUR1)
AGO: AGOS_ADR051C
ERC: Ecym_1184
KLA: KLLA0_E08119g
KMX: KLMA_40362(DUR1)
LTH: KLTH0E12078g
VPO: Kpol_299p2
ZRO: ZYRO0F08976g
CGR: CAGL0M05533g
NCS: NCAS_0B02280(NCAS0B02280)
NDI: NDAI_0C05400(NDAI0C05400)
TPF: TPHA_0L01560(TPHA0L01560)
TDL: TDEL_0B03290(TDEL0B03290)
PIC: PICST_28452(DUR1)
CAL: CAALFM_C104660WA(DUR1)
CAUR: QG37_06077
SLB: AWJ20_5191(DUR1)
ENC: ECL_02193
EEC: EcWSU1_02003(gatA)
ESA: ESA_01767
CSK: ES15_1949
CTU: CTU_21870
KPE: KPK_2627(atzF)
KVA: Kvar_2573
KOX: KOX_20290
KOE: A225_2925
RAO: DSD31_13105(atzF)
LEI: C2U54_15655(atzF)
EBF: D782_1860
EBC: C2U52_27945(atzF)
PSTS: E05_21380
SPE: Spro_1418
SRL: SOD_c13040(atzF)
SPLY: Q5A_007110(atzF)
SMAF: D781_1327
SMAR: SM39_0851
SERF: L085_21510
SERA: Ser39006_007510(atzF)
SERM: CLM71_06500(atzF)
RAA: Q7S_00370
ECA: ECA2141
PATR: EV46_10245
PATO: GZ59_24230
PCT: PC1_2164
PEC: W5S_2477
DDQ: DDI_3235
DFN: CVE23_17460(atzF)
ETA: ETA_06890
EBI: EbC_06400
EGE: EM595_0657(atzF)
EPE: CI789_15265(atzF)
PLF: PANA5342_3668(uahA)
PVA: Pvag_0053(uaha3)
PAGG: AL522_07250(atzF)
PSTW: DSJ_05900
XAC: XAC4327(uahA)
XCI: XCAW_04699(gatA)
XPH: XppCFBP6546_12170(XppCFBP6546P_12170)
PSUW: WQ53_06105
PSD: DSC_01220
PMY: Pmen_0704
PMK: MDS_0789
PPSE: BN5_0595
PCQ: PcP3B5_39100(atzF)
PAMG: BKM19_008735(atzF)
PAVL: BKM03_07635(atzF) BKM03_23020(atzF)
PMAN: OU5_2034
PEN: PSEEN4016
PSA: PST_2005
PKC: PKB_4202(DUR1)
PSES: PSCI_4771
PANR: A7J50_1569
PSET: THL1_5144
PSIL: PMA3_22625
ABY: ABAYE2422
ABN: AB57_1472
ABB: ABBFA_002246(atzF)
ABX: ABK1_1736
ABZ: ABZJ_01447(atzF)
ABH: M3Q_1659
ABAD: ABD1_13110
ABAZ: P795_10995
ABAU: IX87_04005
ASJ: AsACE_CH01428(atzF)
ADV: DJ533_13280(atzF)
ARJ: DOM24_03850(atzF)
PHA: PSHAa1442
MAD: HP15_2883
MARA: D0851_07775(atzF)
SALM: D0Y50_18905(atzF)
CJA: CJA_1244
SDE: Sde_1128
TTU: TERTU_3486(atzF)
MII: BTJ40_07685(atzF)
MPSY: CEK71_06110(atzF)
TCX: Tcr_1793
AEH: Mlg_1917
HHA: Hhal_2362
TGR: Tgr7_3090
HAM: HALO3023
HHH: CLM76_17335(atzF)
HAF: C8233_04080(atzF)
ABO: ABO_2506
ADI: B5T_00741
AXE: P40_03485
KAK: Kalk_13745(atzF)
TOL: TOL_3578
BSAN: CHH28_05040(atzF)
TAU: Tola_2898
SOK: D0B54_13695(atzF)
ACII: C4901_07815(atzF)
SALN: SALB1_0444
CGD: CR3_3919
BVE: AK36_5071(atzF)
BCJ: BCAS0273
BCEN: DM39_3455(atzF)
BCEW: DM40_5321(atzF)
BCEO: I35_7287
BCED: DM42_6768(atzF)
BDL: AK34_5499(atzF)
BCON: NL30_04575
BLAT: WK25_17275
BTEI: WS51_26370
BSEM: WJ12_17610
BPSL: WS57_06395
BMEC: WJ16_26385
BSTG: WT74_18120
BGO: BM43_112(atzF)
BUK: MYA_5127
BXB: DR64_6673(atzF) DR64_6690(atzF)
BPH: Bphy_3980
BFN: OI25_3648(atzF) OI25_7103(atzF)
PARB: CJU94_22270(atzF) CJU94_22355(atzF)
PHS: C2L64_19375(atzF)
PTER: C2L65_17250(atzF)
PGP: CUJ91_24580(atzF)
PAPI: SG18_19930
PLG: NCTC10937_01302(gatA_2)
BPT: Bpet0294(uahA)
BAV: BAV0804
BHO: D560_2863(atzF)
BHM: D558_2841(atzF)
BHZ: ACR54_01300(atzF)
AXY: AXYL_05233(atzF)
AXX: ERS451415_05059(gatA_8)
ASW: CVS48_12660(atzF)
ACHR: C2U31_28915(atzF)
ODI: ODI_R0885
RFR: Rfer_0126
PNA: Pnap_0035
VEI: Veis_0832
VAM: C4F17_17475(atzF)
CTES: O987_22570
HYR: BSY239_964(atzF)
HYB: Q5W_18995
CBAA: SRAA_0220
MPT: Mpe_A0773
MMS: mma_3078(uahA)
HEE: hmeg3_06765(atzF)
LCH: Lcho_3031
THI: THI_3695
NLC: EBAPG3_006865(atzF)
MEH: M301_2217
MEP: MPQ_0993(gatA) MPQ_0994
SLT: Slit_0081
GCA: Galf_0782
DAR: Daro_0075
AZO: azo0044
ACOM: CEW83_09345(atzF) CEW83_17195(atzF)
WSU: WS1118(gatA)
MLO: mlr7070
PLA: Plav_1893
RBS: RHODOSMS8_03365(atzF)
SFD: USDA257_c27410(gatA1)
ARA: Arad_10039(atzF)
AVI: Avi_5649
AGC: BSY240_4746(atzF)
RLG: Rleg_6104
RHT: NT26_0373
BJA: bll1044
BRA: BRADO6814
AOL: S58_06490
BRK: CWS35_10045(atzF)
BOT: CIT37_09080(atzF)
RPB: RPB_4057
RPC: RPC_1134
RPD: RPD_3802
BOS: BSY19_3609(atzF)
VGO: GJW-30_1_01656(atzF)
XAU: Xaut_4705
AZC: AZC_1523
SNO: Snov_3775
MEX: Mext_3721
MPO: Mpop_3899
MET: M446_3691
MOR: MOC_0456(atzF)
META: Y590_18470
MAQU: Maq22A_1p30070(gatA)
METD: C0214_24160(atzF)
MSL: Msil_2607
DEI: C4375_10420(atzF)
BVR: BVIR_3167
HDI: HDIA_3341(atzF_1) HDIA_3396(atzF_2)
RBM: TEF_15415
CCR: CC_1830
CAK: Caul_4450
RSP: RSP_3449
JAN: Jann_2804
RDE: RD1_0244
LVS: LOKVESSMR4R_03018(atzF)
SAGU: CDO87_13225(atzF)
NAR: Saro_0605
NOV: TQ38_016735(atzF)
SPMI: K663_03325
SPHY: CHN51_14500(atzF)
BLAS: BSY18_2817(atzF)
EFV: CHH26_15250(atzF)
PHZ: CHX26_13140(atzF)
GOH: B932_3134
ACR: Acry_2726
APK: APA386B_219(atzF)
ASZ: ASN_560(atzF)
APOM: CPF11_00700(atzF)
ATO: CIW82_09550(atzF)
ACET: DS739_06965(atzF)
RRU: Rru_A1505
AZL: AZL_a09070(gatA) AZL_b03880(gatA)
TMO: TMO_1197
MAGQ: MGMAQ_0576
NCB: C0V82_20995(atzF) C0V82_24165(atzF)
BMD: BMD_0981(atzF)
BMEG: BG04_3269(atzF)
BAG: Bcoa_0868
PPOL: X809_33795
PMW: B2K_03795
PDH: B9T62_31030(atzF)
AAC: Aaci_0767
AAD: TC41_0745
CTYK: CTK_C20910
RIX: RO1_30990
RIM: ROI_33670
SGY: Sgly_0808
MMC: Mmcs_1841
MKM: Mkms_1888
MJL: Mjls_1822
MSM: MSMEG_2189(atzF)
MVQ: MYVA_0190(atzF)
MTHN: 4412656_02192(gatA_2)
MAB: MAB_1558c
MABB: MASS_1653
MCHE: BB28_08515
MSTE: MSTE_01559
ASD: AS9A_0596
CEF: CE0710
NFA: NFA_22180
NFR: ERS450000_01879(gatA_2)
RER: RER_15450
REY: O5Y_07340
ROP: ROP_18540
REQ: REQ_15710
RFA: A3L23_01648(atzF)
RHS: A3Q41_01706(atzF)
RRZ: CS378_09315(atzF)
RHU: A3Q40_01990(atzF)
RQI: C1M55_07820(atzF)
RRT: 4535765_03781(gatA_7)
GBR: Gbro_3887
TPR: Tpau_1595
SRT: Srot_2307
DIT: C3V38_06980(atzF)
DIZ: CT688_00505(atzF)
SBH: SBI_01437
SHY: SHJG_1245
SALS: SLNWT_1829
KSK: KSE_50740
CMI: CMM_2824
CMS: CMS2365
CMC: CMN_02370
ART: Arth_3670
ARN: CGK93_01130(atzF)
ARTH: C3B78_01320(atzF)
AAU: AAur_0186(atzF)
IDO: I598_2367(atzF)
CFL: Cfla_0711
FAL: FRAAL6407
NML: Namu_2026
KRA: Krad_0905
AMD: AMED_2634(amiE)
AMN: RAM_13385
AMM: AMES_2606(amiE)
AMZ: B737_2607(amiE)
ASE: ACPL_6107
AFS: AFR_34350
ACTS: ACWT_5975
CAI: Caci_3729
GVI: glr0961
GLJ: GKIL_0053(gatA)
CAA: Caka_2068
VBH: CMV30_15915(atzF)
VBC: C5Q97_20565(atzF)
SUS: Acid_0865
 » show all
Taxonomy
Reference
1
  Authors
Maitz, G.S., Haas, E.M. and Castric, P.A.
  Title
Purification and properties of the allophanate hydrolase from Chlamydomonas reinhardii.
  Journal
Biochim Biophys Acta 714:486-491 (1982)
Reference
2  [PMID:4556303]
  Authors
Roon RJ, Levenberg B.
  Title
Urea amidolyase. I. Properties of the enzyme from Candida utilis.
  Journal
J Biol Chem 247:4107-13 (1972)
Reference
3  [PMID:6124544]
  Authors
Sumrada RA, Cooper TG.
  Title
Urea carboxylase and allophanate hydrolase are components of a multifunctional protein in yeast.
  Journal
J Biol Chem 257:9119-27 (1982)
Reference
4  [PMID:15796980]
  Authors
Kanamori T, Kanou N, Kusakabe S, Atomi H, Imanaka T.
  Title
Allophanate hydrolase of Oleomonas sagaranensis involved in an ATP-dependent degradation pathway specific to urea.
  Journal
FEMS Microbiol Lett 245:61-5 (2005)
DOI:10.1016/j.femsle.2005.02.023
Reference
5  [PMID:16085834]
  Authors
Cheng G, Shapir N, Sadowsky MJ, Wackett LP.
  Title
Allophanate hydrolase, not urease, functions in bacterial cyanuric acid metabolism.
  Journal
Appl Environ Microbiol 71:4437-45 (2005)
DOI:10.1128/AEM.71.8.4437-4445.2005
Reference
6  [PMID:15901697]
  Authors
Shapir N, Sadowsky MJ, Wackett LP.
  Title
Purification and characterization of allophanate hydrolase (AtzF) from Pseudomonas sp. strain ADP.
  Journal
J Bacteriol 187:3731-8 (2005)
DOI:10.1128/JB.187.11.3731-3738.2005
  Sequence
Reference
7  [PMID:16597948]
  Authors
Shapir N, Cheng G, Sadowsky MJ, Wackett LP.
  Title
Purification and characterization of TrzF: biuret hydrolysis by allophanate hydrolase supports growth.
  Journal
Appl Environ Microbiol 72:2491-5 (2006)
DOI:10.1128/AEM.72.4.2491-2495.2006
Other DBs
ExplorEnz - The Enzyme Database: 3.5.1.54
IUBMB Enzyme Nomenclature: 3.5.1.54
ExPASy - ENZYME nomenclature database: 3.5.1.54
UM-BBD (Biocatalysis/Biodegradation Database): 3.5.1.54
BRENDA, the Enzyme Database: 3.5.1.54
CAS: 9076-72-6

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