KEGG   ENZYME: 3.5.1.77Help
Entry
EC 3.5.1.77                 Enzyme                                 

Name
N-carbamoyl-D-amino-acid hydrolase;
D-N-carbamoylase;
N-carbamoylase (ambiguous);
N-carbamoyl-D-amino acid hydrolase
Class
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In linear amides
BRITE hierarchy
Sysname
N-carbamoyl-D-amino-acid amidohydrolase
Reaction(IUBMB)
an N-carbamoyl-D-amino acid + H2O = a D-amino acid + NH3 + CO2 [RN:R02190]
Reaction(KEGG)
Substrate
N-carbamoyl-D-amino acid [CPD:C05226];
H2O [CPD:C00001]
Product
D-amino acid [CPD:C00405];
NH3 [CPD:C00014];
CO2 [CPD:C00011]
Comment
This enzyme, along with EC 3.5.1.87 (N-carbamoyl-L-amino-acid hydrolase), EC 5.1.99.5 (hydantoin racemase) and hydantoinase, forms part of the reaction cascade known as the "hydantoinase process", which allows the total conversion of D,L-5-monosubstituted hydantoins into optically pure D- or L-amino acids [2]. It has strict stereospecificity for N-carbamoyl-D-amino acids and does not act upon the corresponding L-amino acids or on the N-formyl amino acids, N-carbamoyl-sarcosine, -citrulline, -allantoin and -ureidopropanoate, which are substrates for other amidohydrolases.
History
EC 3.5.1.77 created 1999, modified 2008
Orthology
K01459  N-carbamoyl-D-amino-acid hydrolase
Genes
GQU: AWC35_02065 AWC35_21320
BGJ: AWC36_06180 AWC36_10120
LBQ: CKQ53_07460 CKQ53_16475
PAO: Pat9b_4333
HAA: A5892_06310
RPJ: N234_35240
REU: Reut_B4535
CNC: CNE_BB1p08270
CCUP: BKK81_02680
CUP: BKK80_02025
PSPU: NA29_16365
PAPI: SG18_12590
BPT: Bpet2330
BAV: BAV0674
AXY: AXYL_03527(dcaS2)
AFQ: AFA_08930
POL: Bpro_1497
PNA: Pnap_4069
AAA: Acav_1139
RTA: Rta_13990
MMED: Mame_04417
HDI: HDIA_3985
RSP: RSP_3461
KVU: EIO_3237
RHC: RGUI_1328
AHT: ANTHELSMS3_01544(amiF)
GXY: GLX_01010
 » show all
Taxonomy
Reference
1  [PMID:8462543]
  Authors
Ogawa J, Shimizu S, Yamada H.
  Title
N-carbamoyl-D-amino acid amidohydrolase from Comamonas sp. E222c purification and characterization.
  Journal
Eur J Biochem 212:685-91 (1993)
DOI:10.1111/j.1432-1033.1993.tb17706.x
Reference
2  [PMID:11849938]
  Authors
Altenbuchner J, Siemann-Herzberg M, Syldatk C
  Title
Hydantoinases and related enzymes as biocatalysts for the synthesis of unnatural chiral amino acids.
  Journal
Curr Opin Biotechnol 12:559-63 (2001)
DOI:10.1016/S0958-1669(01)00263-4
Other DBs
ExplorEnz - The Enzyme Database: 3.5.1.77
IUBMB Enzyme Nomenclature: 3.5.1.77
ExPASy - ENZYME nomenclature database: 3.5.1.77
BRENDA, the Enzyme Database: 3.5.1.77
CAS: 71768-08-6

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