KEGG   ENZYME: 3.5.1.81Help
Entry
EC 3.5.1.81                 Enzyme                                 

Name
N-acyl-D-amino-acid deacylase
Class
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In linear amides
BRITE hierarchy
Sysname
N-acyl-D-amino acid amidohydrolase
Reaction(IUBMB)
N-acyl-D-amino acid + H2O = a carboxylate + D-amino acid [RN:R02192]
Reaction(KEGG)
Substrate
N-acyl-D-amino acid [CPD:C06378];
H2O [CPD:C00001]
Product
carboxylate [CPD:C00060];
D-amino acid [CPD:C00405]
Comment
The enzyme from Alcaligenes denitrificans subsp. xylosoxydans and Alcaligenes xylosoxydans subsp. xylosoxydans has wide specificity; hydrolyses N-acyl derivative of neutral D-amino acids. Used in separating D- and L-amino acids. Requires zinc.
History
EC 3.5.1.81 created 1999
Orthology
K06015  N-acyl-D-amino-acid deacylase
Genes
ECM: EcSMS35_4896
EFE: EFER_3059
KPN: KPN_04793
KPU: KP1_0751
KPM: KPHS_06430
KPP: A79E_4356
KPH: KPNIH24_03300
KPZ: KPNIH27_03175
KPV: KPNIH29_03500
KPW: KPNIH30_03460
KPT: VK055_2644(dan)
KPR: KPR_0873
KPJ: N559_4500
KPX: PMK1_02254(dan)
KPNU: LI86_23125
KPNK: BN49_4400
KOX: KOX_10075
KOE: A225_0717
EAE: EAE_10420
EAR: CCG31910
CRO: ROD_48401
SPE: Spro_0551
SMAF: D781_2939
SOD: Sant_3073(dan)
TPTY: NCTC11468_02887(dan)
SACZ: AOT14_07750(dan)
LAB: LA76x_0723(dan)
LAQ: GLA29479_2932(dan)
LGU: LG3211_4572(dan)
VSP: VS_II0036
VNI: VIBNI_A3114(dan)
PSYR: N018_01975
PPRC: PFLCHA0_c07860(dan)
PPRO: PPC_0788
PFS: PFLU_5178
PFB: VO64_2019
PMAN: OU5_2732
PSEM: TO66_03720
PSOS: POS17_0765
PANR: A7J50_4922
PSIL: PMA3_07975
SHL: Shal_3762
PSEO: OM33_20335
PSPO: PSPO_b1261
PSEN: PNC201_20375(dan)
CSA: Csal_0091
HAM: HALO3074
RSO: RSp1187(ndeD)
RSN: RSPO_m00833(ndeD)
RSE: F504_4683
RPI: Rpic_4195
REH: H16_B0531(h16_B0531) H16_B1123(h16_B1123) H16_B1209(h16_B1209) H16_B2124(h16_B2124)
BMA: BMA0178
BMAL: DM55_1582
BMAE: DM78_411
BMAQ: DM76_1561
BMAI: DM57_2214
BMAF: DM51_3153
BMAZ: BM44_899
BMAB: BM45_504
BPS: BPSL0630(dan)
BPSE: BDL_1374
BPSM: BBQ_2797
BPSU: BBN_2920
BPSD: BBX_3321
BPZ: BP1026B_I2895(dan)
BPK: BBK_855
BPSH: DR55_463
BPSA: BBU_1521
BPSO: X996_70
BTE: BTH_I0546
BTQ: BTQ_568
BTJ: BTJ_1917
BTZ: BTL_3153
BTD: BTI_3158
BTV: BTHA_3436
BTHE: BTN_1559
BTHM: BTRA_31
BTHA: DR62_1746
BTHL: BG87_3346
BOK: DM82_58
BOC: BG90_963
BVE: AK36_1157
BCJ: BCAL0866(dan) BCAM2259(dan)
BCEN: DM39_2830 DM39_6572(dan)
BCEW: DM40_356
BCEO: I35_2803(dan) I35_6152(dan)
BMK: DM80_2176 DM80_6479(dan)
BMUL: NP80_2873 NP80_5241(dan)
BCT: GEM_0702
BCED: DM42_2333 DM42_5732(dan)
BDL: AK34_349
BCON: NL30_26785
BUB: BW23_2155
BLAT: WK25_13275
BTEI: WS51_24070
BSEM: WJ12_13705
BSTG: WT74_14510
BGU: KS03_544
BGO: BM43_943
BUK: MYA_2498
BUL: BW21_3307
BXB: DR64_1735
BPH: Bphy_0366
BFN: OI25_1070
PPNM: LV28_18200
PPUL: RO07_12495
PSPU: NA29_08575
PAPI: SG18_12825
PLG: NCTC10937_01912(dan)
BBH: BN112_3094(ptpX)
BAV: BAV0959(dan)
BHZ: ACR54_01042(dan_1) ACR54_01730(dan_2)
BTRM: SAMEA390648702056(dan)
AMIM: MIM_c30540
ODI: ODI_R4081
AAA: Acav_1137
HYB: Q5W_24630
HSE: Hsero_0570(ndeD) Hsero_1750(ndeD)
HRB: Hrubri_1621(ndeD)
CFU: CFU_1275(dan)
CARE: LT85_3491
GEO: Geob_0285
DDE: Dde_1269
DPI: BN4_11190
SCL: sce1547
BRA: BRADO6610(dan)
BBT: BBta_0926(dan)
AOL: S58_09940
BRO: BRAD285_6667(dan)
RBM: TEF_18550
CAK: Caul_1130
JAN: Jann_3245
SAL: Sala_1467
SPHM: G432_10805
SPHI: TS85_04295
BLAS: BSY18_2947
AAY: WYH_00893(dan_1)
PMW: B2K_33055
CBE: Cbei_4937
CBZ: Cbs_4937
CSQ: CSCA_4997
CPY: Cphy_1501
STH: STH3170
DSY: DSY1804
DHD: Dhaf_2958
DRM: Dred_0263
TMR: Tmar_2329
BPRS: CK3_17970
TPZ: Tph_c09230(dan1) Tph_c27030(dan2)
PFT: JBW_03546
ROP: ROP_21890
REQ: REQ_04900
RHB: NY08_171
RFA: A3L23_03556(dag_2)
RHS: A3Q41_04670(dag_2)
RHU: A3Q40_00710(dan)
TPR: Tpau_3006
SCO: SCO4986(2SCK36.09c)
SGR: SGR_2547
SGB: WQO_22745
SCT: SCAT_3867
SFA: Sfla_2279
SBH: SBI_04231
SHY: SHJG_6091
SVE: SVEN_4666
SALB: XNR_4068
SALS: SLNWT_6729
STRP: F750_4526
SFI: SFUL_4789
SALU: DC74_5164
SALL: SAZ_27460
STRE: GZL_03682
SLD: T261_3051
STRM: M444_21865
SPRI: SPRI_2873
SRW: TUE45_05517(dan_2)
SLE: sle_27090(sle_27090)
STRD: NI25_14010
SMAL: SMALA_4833
SLAU: SLA_4852
SALJ: SMD11_2528
SFK: KY5_5126c
KSK: KSE_64900
ARR: ARUE_c42040(dan)
ARM: ART_0022
AAU: AAur_4061
LMOI: VV02_16760
IDO: I598_1266
MPH: MLP_33450
KFL: Kfla_6164
NDA: Ndas_5490
NAL: B005_1609
KRA: Krad_1032
AMD: AMED_6064
AMN: RAM_31090
AMM: AMES_5979
AMZ: B737_5979
AOI: AORI_5079
PDX: Psed_3083
PSEA: WY02_20665
PSEE: FRP1_22660
PSEH: XF36_20995
AMI: Amir_5481
SESP: BN6_66240
KAL: KALB_6487
CAI: Caci_0720
SNA: Snas_2530
RBA: RB2098(ndaD)
PSL: Psta_1927
FMR: Fuma_02525 Fuma_03998(dan_1) Fuma_04847(dan_2)
IPA: Isop_0277
PBOR: BSF38_04880(dan)
KST: KSMBR1_0179(dan)
VBL: L21SP4_02365(dan)
ABAS: ACPOL_3510
SUS: Acid_3545
ABAC: LuPra_01656(dan_1) LuPra_02316(dan_2) LuPra_05793(dan_3)
TLI: Tlie_0010
RMR: Rmar_1358
LBY: Lbys_1896
FAE: FAES_5424
HSW: Hsw_0996
MLT: VC82_2831
MOX: DAMO_1463(dan)
PYN: PNA2_0671
HMA: pNG7249(ureX)
HHI: HAH_5304(ureX)
NMG: Nmag_3660
 » show all
Taxonomy
Reference
1  [PMID:8541651]
  Authors
Wakayama M, Katsuno Y, Hayashi S, Miyamoto Y, Sakai K, Moriguchi M.
  Title
Cloning and sequencing of a gene encoding D-aminoacylase from Alcaligenes xylosoxydans subsp. xylosoxydans A-6 and expression of the gene in Escherichia coli.
  Journal
Biosci Biotechnol Biochem 59:2115-9 (1995)
DOI:10.1271/bbb.59.2115
  Sequence
Reference
2  [PMID:8776758]
  Authors
Wakayama M, Hayashi S, Yatsuda Y, Katsuno Y, Sakai K, Moriguchi M.
  Title
Overproduction of D-aminoacylase from Alcaligenes xylosoxydans subsp. xylosoxydans A-6 in Escherichia coli and its purification.
  Journal
Protein Expr Purif 7:395-9 (1996)
DOI:10.1006/prep.1996.0059
Other DBs
ExplorEnz - The Enzyme Database: 3.5.1.81
IUBMB Enzyme Nomenclature: 3.5.1.81
ExPASy - ENZYME nomenclature database: 3.5.1.81
BRENDA, the Enzyme Database: 3.5.1.81
CAS: 65979-42-2

DBGET integrated database retrieval system