KEGG   ENZYME: 3.5.1.87Help
Entry
EC 3.5.1.87                 Enzyme                                 

Name
N-carbamoyl-L-amino-acid hydrolase;
N-carbamyl L-amino acid amidohydrolase;
N-carbamoyl-L-amino acid amidohydrolase;
L-N-carbamoylase;
N-carbamoylase (ambiguous)
Class
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In linear amides
BRITE hierarchy
Sysname
N-carbamoyl-L-amino-acid amidohydrolase
Reaction(IUBMB)
an N-carbamoyl-L-2-amino acid (a 2-ureido carboxylate) + H2O = an L-2-amino acid + NH3 + CO2 [RN:R05782]
Reaction(KEGG)
R05782;
(other) R00254
Show
Substrate
N-carbamoyl-L-2-amino acid [CPD:C11529];
H2O [CPD:C00001]
Product
L-2-amino acid [CPD:C00151];
NH3 [CPD:C00014];
CO2 [CPD:C00011]
Comment
This enzyme, along with EC 3.5.1.77 (N-carbamoyl-D-amino-acid hydrolase), EC 5.1.99.5 (hydantoin racemase) and hydantoinase, forms part of the reaction cascade known as the "hydantoinase process", which allows the total conversion of D,L-5-monosubstituted hydantoins into optically pure D- or L-amino acids [3]. The enzyme from Alcaligenes xylosoxidans has broad specificity for carbamoyl-L-amino acids, although it is inactive on the carbamoyl derivatives of glutamate, aspartate, arginine, tyrosine or tryptophan. The enzyme from Sinorhizobium meliloti requires a divalent cation for activity and can hydrolyse N-carbamoyl-L-tryptophan as well as N-carbamoyl L-amino acids with aliphatic substituents [2]. The enzyme is inactive on derivatives of D-amino acids. In addition to N-carbamoyl L-amino acids, the enzyme can also hydrolyse formyl and acetyl derivatives to varying degrees [1,2].
History
EC 3.5.1.87 created 2001, modified 2008
Orthology
K06016  beta-ureidopropionase / N-carbamoyl-L-amino-acid hydrolase
Genes
EAS: Entas_1441 Entas_2233
EAU: DI57_07170
ECAN: CWI88_11300
ERN: BFV67_11270
ENX: NI40_011705
ENF: AKI40_3727
KPN: KPN_00569 KPN_00965
KPU: KP1_1508 KP1_1935
KPM: KPHS_18440
KPP: A79E_3276 A79E_3676
KPE: KPK_4016
KPR: KPR_3617
KPJ: N559_3316
KPX: PMK1_03298(amaB_1)
KPNU: LI86_17230
KVA: Kvar_3805
KOX: KOX_13725
KOE: A225_1564
CKO: CKO_02939
YEN: YE0805
YEW: CH47_2682
YRO: CH64_3420
SPE: Spro_2555
SRL: SOD_c24190(amaB)
SPLY: Q5A_013435(amaB)
SMAR: SM39_1987
SERF: L085_16010
ETA: ETA_27480(amaB)
TPTY: NCTC11468_00688(amaB) NCTC11468_02244(allC)
PMR: PMI0287
MMK: MU9_100
HIN: HI0588
PMU: PM0160
PMP: Pmu_11530
PMUL: DR93_1906
MSU: MS1555(argE)
MVE: X875_8880
ASU: Asuc_1061
PAE: PA0444
PAEV: N297_454
PAEI: N296_454
PAU: PA14_05810(amaB)
PNC: NCGM2_5753(amaB)
PAEB: NCGM1900_0454(amaB)
PAEP: PA1S_02250
PAEM: U769_02280
PAEL: T223_02240
PAEG: AI22_01655
PAEC: M802_453
PAEO: M801_454
PPSE: BN5_0332
PPU: PP_0614 PP_4034(hyuC)
PSB: Psyr_3056
PSYR: N018_11205
PPRC: PFLCHA0_c26130(hyuC1) PFLCHA0_c37230(hyuC2) PFLCHA0_c41980(amaB)
PMAN: OU5_6140
PEN: PSEEN3255
PSES: PSCI_4602
PSOS: POS17_2552
ABAD: ABD1_05490(allC)
SPSW: Sps_03941
ILO: IL2574
CPS: CPS_4042
MBS: MRBBS_2244(pucF) MRBBS_2283(amaB)
MICC: AUP74_00935(amaB)
NOC: Noc_0781
NHL: Nhal_2225
NWA: Nwat_2327
TEE: Tel_05940
TGR: Tgr7_0180
TKM: TK90_0027
TNI: TVNIR_3753(amaB_[H])
TVR: TVD_13765
GAI: IMCC3135_08120(amaB)
HBE: BEI_0322(pydC)
KKO: Kkor_2055
KGE: TQ33_0500
RSE: F504_2625
RPI: Rpic_4903
REH: H16_A1475(h16_A1475) H16_A1734(h16_A1734) H16_A3589(h16_A3589) H16_B2014(h16_B2014) H16_B2069(h16_B2069)
RME: Rmet_1622(amaB-1) Rmet_1931(amaB-2) Rmet_1945 Rmet_4480(amaB-3)
BMA: BMA2217(amaB-1)
BMV: BMASAVP1_A0702(amaB-1)
BML: BMA10229_A2536(amaB)
BMN: BMA10247_2078(amaB)
BMAL: DM55_2260
BMAE: DM78_2496
BMAQ: DM76_2244
BMAI: DM57_983
BMAF: DM51_1863
BMAZ: BM44_1150
BMAB: BM45_716
BPD: BURPS668_1147(amaB_2)
BDL: AK34_1910
PLG: NCTC10937_02676(amaB) NCTC10937_02816(hyuC2)
BPE: BP1859(amaB) BP2301
BPC: BPTD_1835(amaB) BPTD_2260
BPER: BN118_0767 BN118_1260(amaB)
BPET: B1917_1653(amaB) B1917_2209
BPEU: Q425_16100 Q425_17160(amaB)
BPA: BPP0686(amaB) BPP1366(amaB) BPP1570(amaB) BPP2133 BPP2428
BBR: BB0693(amaB) BB1530 BB1877 BB2432(amaB) BB2648(amaB) BB4730
BPT: Bpet2340(amaB1) Bpet2917(amaB2) Bpet2960(amaB3)
BAV: BAV2202(amaB)
BHZ: ACR54_02098(amaB_1) ACR54_02295(amaB_2) ACR54_02444(amaB_3) ACR54_02959(amaB_4)
AXX: ERS451415_01540(amaB_1) ERS451415_02059(amaB_2) ERS451415_02189(amaB_3) ERS451415_05477(amaB_4)
PUT: PT7_3197
AJS: Ajs_1115
ACRA: BSY15_565(uraD)
RTA: Rta_32660
LIM: L103DPR2_01152(amaB)
LIH: L63ED372_00444(amaB)
HYB: Q5W_15085
SIMP: C6571_12190(uraD)
MELM: C7H73_12290(uraD)
MPT: Mpe_A0776
HAR: HEAR3252(amaB)
MMS: mma_0032(amaB1) mma_0662(amaB2)
JAG: GJA_4391(uraD) GJA_4394
LCH: Lcho_1099
TIN: Tint_2243
THI: THI_2594 THI_3625(atcC)
CGRA: CGRAC_0113
CURE: CUREO_0627
CSPF: CSF_1614
SDL: Sdel_0746
HOH: Hoch_6670
AMIH: CO731_02738(amaB_1) CO731_05545(amaB_2)
SMD: Smed_2366
RHI: NGR_c24830(amaB)
SFD: USDA257_c24450(amaB1) USDA257_c49140(amaB2)
EAD: OV14_3665(amaB) OV14_4011(amaB) OV14_a0300(amaB) OV14_a1130(amaB) OV14_b0513(amaB)
ATU: Atu2385(amaB)
AVI: Avi_3455(amaB) Avi_7539(amaB)
RLE: RL0367(amaB) RL3716(atcC) pRL120159
RHL: LPU83_0432(amaB1) LPU83_3164(amaB3) LPU83_pLPU83d1276(amaB)
RHT: NT26_2524
RHK: Kim5_CH00369 Kim5_CH02260(amaB-1) Kim5_CH03565(amaB-2)
BME: BMEI1643
BMEL: DK63_1848
BMEE: DK62_1103
BMF: BAB1_0310
BABO: DK55_330
BABR: DO74_1557
BABT: DK49_93
BABB: DK48_1796
BABU: DK53_320
BABS: DK51_1145
BABC: DO78_237
BMS: BR0279
BSZ: DK67_1225
BOV: BOV_0293
BCAR: DK60_378
BCAS: DA85_01375
BMR: BMI_I285
BPP: BPI_I314
BPV: DK65_1061
OAN: Oant_0348
OAH: DR92_2592
BJA: bll2919(amaB) blr6249
RPA: RPA1745
RPB: RPB_3623
RPC: RPC_1668
RPD: RPD_1844
RPT: Rpal_1945
OCA: OCAR_6699
OCG: OCA5_c13690(amaB)
OCO: OCA4_c13690(amaB)
XAU: Xaut_3376
MPO: Mpop_4007
PHL: KKY_2262
MMED: Mame_00019(amaB_1) Mame_01297(amaB_2)
HDI: HDIA_1466(amaB_1) HDIA_3087 HDIA_3257 HDIA_4555(amaB_3)
SIL: SPO1781
RCP: RCAP_rcc02526(amaB)
JAN: Jann_2707
RDE: RD1_3101
DSH: Dshi_1367(amaB)
KVU: EIO_1008(amaB)
KVL: KVU_0520(amaB)
KRO: BVG79_00784(pydC)
PSF: PSE_2795
PGA: PGA1_c12840(amaB)
PGL: PGA2_c12810(amaB)
PGD: Gal_02120
PHP: PhaeoP97_01282(amaB)
PPIC: PhaeoP14_01204(amaB)
OAT: OAN307_c41940(amaB)
OTM: OSB_04370(amaB)
PTP: RCA23_c08660(amaB)
RHC: RGUI_0787
RMM: ROSMUCSMR3_01488(amaB)
LVS: LOKVESSMR4R_03342(amaB)
SSAN: NX02_15060
GOH: B932_1610
ALI: AZOLI_p30403(amaB2) AZOLI_p40508(amaB3)
TMO: TMO_b0181(allC) TMO_c0379(hyuC)
TXI: TH3_13450
BMP: NG74_00532(amaB)
BAO: BAMF_1010
BQL: LL3_00995
BQY: MUS_0514(amaB)
BAMI: KSO_016950
BAMF: U722_02770
BMQ: BMQ_2659
BMEG: BG04_5114
BAG: Bcoa_0946
BACB: OY17_05395
BEO: BEH_13360
BGY: BGLY_1708
GEA: GARCT_01730(amaB)
LSP: Bsph_2007
HHD: HBHAL_2397(amaB)
SXO: SXYL_02497(amaB)
PPY: PPE_02234
PMW: B2K_08945
PRI: PRIO_0355
SIV: SSIL_0755
CBO: CBO0629
CBA: CLB_0669(atcC)
CBH: CLC_0684
CBY: CLM_0737(atcC)
CBB: CLD_0128(atcC)
CBF: CLI_0709
CBM: CBF_0679
CBE: Cbei_1944
CBZ: Cbs_1944
CBEI: LF65_02111
CKL: CKL_2403(amaB)
CKR: CKR_2119
CLJ: CLJU_c35910(amaB2)
CPAS: Clopa_1051
CPAT: CLPA_c11060(amaB)
CPAE: CPAST_c11060(amaB)
CSQ: CSCA_2143
CTYK: CTK_C12540(allC)
ESR: ES1_25610
ESU: EUS_26750
RCH: RUM_00540
BPRL: CL2_22210
CPRO: CPRO_21530(pucF)
DSY: DSY4355
EHL: EHLA_1846
TMR: Tmar_1802
SAY: TPY_0092
TSH: Tsac_2218
VPR: Vpar_0154
PUF: UFO1_2806
PFT: JBW_02711
AIN: Acin_0712
MVQ: MYVA_3904
CAR: cauri_0627(amaB)
CVA: CVAR_1015
CTER: A606_03940
CSP: WM42_0739
CAMG: CAMM_03700
CMIN: NCTC10288_01899(amaB)
NFA: NFA_21000
NFR: ERS450000_02003(amaB)
ROP: ROP_25390
RHB: NY08_102
RRT: 4535765_01837(amaB)
SCO: SCO3072(SCE25.13c)
SALB: XNR_1970
SGR: SGR_4463
SGB: WQO_13220
SCT: SCAT_2087
SFA: Sfla_3836
SHY: SHJG_4537
SVE: SVEN_2821
SALS: SLNWT_2614
STRP: F750_2898
SFI: SFUL_2673
SALU: DC74_3512
SALL: SAZ_18680
STRE: GZL_05388
SLD: T261_5029
STRM: M444_14455
SPRI: SPRI_4479
SRW: TUE45_04593(amaB)
SLE: sle_42050(sle_42050)
SRN: A4G23_02088(amaB)
STRD: NI25_23420
SMAL: SMALA_3173
SLAU: SLA_2800
SALJ: SMD11_4155
SLX: SLAV_22695(amaB)
SFK: KY5_3257c
KSK: KSE_29690
MTS: MTES_1773
ARR: ARUE_c39850(amaB)
ARM: ART_2655
AAU: AAur_0749
ACH: Achl_3520
KRH: KRH_00700
LMOI: VV02_04860
CFI: Celf_0072
SERJ: SGUI_2337
PAC: PPA2167
PAW: PAZ_c22480(amaB)
PACC: PAC1_11020
PACH: PAGK_2064
CACN: RN83_11070
CGRN: 4412665_00284(amaB_1) 4412665_01163(amaB_2)
MPH: MLP_06230
NCA: Noca_4387
NDK: I601_0967(amaB)
KFL: Kfla_6772
PSIM: KR76_15470
TFU: Tfu_2012
TCU: Tcur_3617
SRO: Sros_1779
NML: Namu_1422
KRA: Krad_4127
SEN: SACE_6405
AOI: AORI_1264
PSEE: FRP1_28355
AMI: Amir_6264
SESP: BN6_75380
KAL: KALB_7844
SAQ: Sare_0999
MIL: ML5_1428
AMS: AMIS_9530
ASE: ACPL_1176
ACTN: L083_1289
AFS: AFR_06420
ACTS: ACWT_1057
CAI: Caci_0252
SNA: Snas_5774
AFO: Afer_1412
SYW: SYNW2452
SYNR: KR49_12955
SYND: KR52_02815
SYNW: SynWH8103_02834(amaB)
CYI: CBM981_1112(amaB)
LET: O77CONTIG1_00963(amaB_1) O77CONTIG1_00964(amaB_2)
CTHE: Chro_0936
CAP: CLDAP_27460(amaB)
DGO: DGo_CA0633(argE)
TRA: Trad_1204
MRB: Mrub_1881
MIN: Minf_0624(argE)
RBA: RB13304(amaB)
ABAS: ACPOL_1945
SUS: Acid_4051
ABAC: LuPra_05393(amaB_2)
SBR: SY1_07900
GAU: GAU_3178
GBA: J421_1410
RMR: Rmar_2091
SLI: Slin_5111
FLM: MY04_1480
HMA: pNG7248(amaB)
HHI: HAH_5305(amaB)
NMO: Nmlp_3636
HVO: HVO_A0295(amaB2) HVO_A0341(amaB3) HVO_B0306(amaB4)
HTU: Htur_3946
SSO: SSO2719(amaB)
SOL: Ssol_0531
SSOA: SULA_0520
SSOL: SULB_0522
SSOF: SULC_0520
SAI: Saci_2132(amaB)
SID: M164_2582
SII: LD85_2911
SIH: SiH_2538
SIR: SiRe_2480
SIC: SiL_2432
VMO: VMUT_1157
 » show all
Taxonomy
Reference
1  [PMID:8590654]
  Authors
Ogawa J, Miyake H, Shimizu S.
  Title
Purification and characterization of N-carbamoyl-L-amino acid amidohydrolase with broad substrate specificity from Alcaligenes xylosoxidans.
  Journal
Appl Microbiol Biotechnol 43:1039-43 (1995)
Reference
2  [PMID:16254442]
  Authors
Martinez-Rodriguez S, Clemente-Jimenez JM, Rodriguez-Vico F, Las Heras-Vazquez FJ
  Title
Molecular cloning and biochemical characterization of L-N-carbamoylase from Sinorhizobium meliloti CECT4114.
  Journal
J Mol Microbiol Biotechnol 9:16-25 (2005)
DOI:10.1159/000088142
  Sequence
[sme:SMc01820]
Reference
3  [PMID:11849938]
  Authors
Altenbuchner J, Siemann-Herzberg M, Syldatk C
  Title
Hydantoinases and related enzymes as biocatalysts for the synthesis of unnatural chiral amino acids.
  Journal
Curr Opin Biotechnol 12:559-63 (2001)
DOI:10.1016/S0958-1669(01)00263-4
Other DBs
ExplorEnz - The Enzyme Database: 3.5.1.87
IUBMB Enzyme Nomenclature: 3.5.1.87
ExPASy - ENZYME nomenclature database: 3.5.1.87
BRENDA, the Enzyme Database: 3.5.1.87

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