KEGG   ENZYME: 3.5.1.93Help
Entry
EC 3.5.1.93                 Enzyme                                 

Name
glutaryl-7-aminocephalosporanic-acid acylase;
7beta-(4-carboxybutanamido)cephalosporanic acid acylase;
cephalosporin C acylase;
glutaryl-7-ACA acylase;
CA;
GCA;
GA;
cephalosporin acylase;
glutaryl-7-aminocephalosporanic acid acylase;
GL-7-ACA acylase
Class
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In linear amides
BRITE hierarchy
Sysname
(7R)-7-(4-carboxybutanamido)cephalosporanate amidohydrolase
Reaction(IUBMB)
(7R)-7-(4-carboxybutanamido)cephalosporanate + H2O = (7R)-7-aminocephalosporanate + glutarate [RN:R07402]
Reaction(KEGG)
Substrate
(7R)-7-(4-carboxybutanamido)cephalosporanate [CPD:C15666];
H2O [CPD:C00001]
Product
(7R)-7-aminocephalosporanate [CPD:C07756];
glutarate [CPD:C00489]
Comment
Forms 7-aminocephalosporanic acid, a key intermediate in the synthesis of cephem antibiotics. It reacts only weakly with cephalosporin C.
History
EC 3.5.1.93 created 2005
Pathway
ec00311  Penicillin and cephalosporin biosynthesis
ec01130  Biosynthesis of antibiotics
Orthology
K12748  glutaryl-7-aminocephalosporanic-acid acylase
Reference
1  [PMID:7607251]
  Authors
Ishii Y, Saito Y, Fujimura T, Sasaki H, Noguchi Y, Yamada H, Niwa M, Shimomura K.
  Title
High-level production, chemical modification and site-directed mutagenesis of a cephalosporin C acylase from Pseudomonas strain N176.
  Journal
Eur J Biochem 230:773-8 (1995)
DOI:10.1111/j.1432-1033.1995.0773h.x
Reference
2  [PMID:10739919]
  Authors
Kinoshita T, Tada T, Saito Y, Ishii Y, Sato A, Murata M.
  Title
Crystallization and preliminary X-ray analysis of cephalosporin C acylase from Pseudomonas sp. strain N176.
  Journal
Acta Crystallogr D Biol Crystallogr 56 ( Pt 4):458-9 (2000)
Reference
3  [PMID:10972935]
  Authors
Monti D, Carrea G, Riva S, Baldaro E, Frare G.
  Title
Characterization of an industrial biocatalyst: immobilized glutaryl-7-ACA acylase.
  Journal
Biotechnol Bioeng 70:239-44 (2000)
Reference
4  [PMID:10945972]
  Authors
Kwon TH, Rhee S, Lee YS, Park SS, Kim KH.
  Title
Crystallization and preliminary X-Ray diffraction analysis of glutaryl-7-aminocephalosporanic acid acylase from Pseudomonas sp. GK16.
  Journal
J Struct Biol 131:79-81 (2000)
DOI:10.1006/jsbi.2000.4256
Reference
5  [PMID:11080627]
  Authors
Kim Y, Yoon K, Khang Y, Turley S, Hol WG.
  Title
The 2.0 A crystal structure of cephalosporin acylase.
  Journal
Structure Fold Des 8:1059-68 (2000)
DOI:10.1016/S0969-2126(00)00505-0
  Sequence
Reference
6  [PMID:11782466]
  Authors
Huang X, Zeng R, Ding X, Mao X, Ding Y, Rao Z, Xie Y, Jiang W, Zhao G.
  Title
Affinity alkylation of the Trp-B4 residue of the beta -subunit of the glutaryl 7-aminocephalosporanic acid acylase of Pseudomonas sp. 130.
  Journal
J Biol Chem 277:10256-64 (2002)
DOI:10.1074/jbc.M108683200
Reference
7  [PMID:12680762]
  Authors
Kim JK, Yang IS, Rhee S, Dauter Z, Lee YS, Park SS, Kim KH.
  Title
Crystal structures of glutaryl 7-aminocephalosporanic acid acylase: insight into autoproteolytic activation.
  Journal
Biochemistry 42:4084-93 (2003)
DOI:10.1021/bi027181x
Other DBs
ExplorEnz - The Enzyme Database: 3.5.1.93
IUBMB Enzyme Nomenclature: 3.5.1.93
ExPASy - ENZYME nomenclature database: 3.5.1.93
BRENDA, the Enzyme Database: 3.5.1.93
CAS: 56645-46-6

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