KEGG   ENZYME: 3.5.5.5Help
Entry
EC 3.5.5.5                  Enzyme                                 

Name
arylacetonitrilase
Class
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In nitriles
BRITE hierarchy
Sysname
arylacetonitrile aminohydrolase
Reaction(IUBMB)
4-chlorophenylacetonitrile + 2 H2O = 4-chlorophenylacetate + NH3 [RN:R04140]
Reaction(KEGG)
R04140;
(other) R00540
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Substrate
4-chlorophenylacetonitrile [CPD:C03679];
H2O [CPD:C00001]
Product
4-chlorophenylacetate [CPD:C03077];
NH3 [CPD:C00014]
Comment
Requires thiol compounds. Also hydrolyses other 4-substituted phenylacetonitriles, thien-2-ylacetonitrile, tolylacetonitriles, and, more slowly, benzyl cyanide.
History
EC 3.5.5.5 created 1992
Orthology
K20766  arylacetonitrilase
Genes
AFA: UZ73_05730
Taxonomy
Reference
1
  Authors
Mauger, J., Nagasawa, T. and Yamada, H.
  Title
Occurrence of a novel nitrilase, arylacetonitrilase, in Alcaligenes faecalis JM3.
  Journal
Arch Microbiol 155:1-6 (1990)
Reference
2  [PMID:2269298]
  Authors
Nagasawa T, Mauger J, Yamada H.
  Title
A novel nitrilase, arylacetonitrilase, of Alcaligenes faecalis JM3. Purification and characterization.
  Journal
Eur J Biochem 194:765-72 (1990)
DOI:10.1111/j.1432-1033.1990.tb19467.x
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 3.5.5.5
IUBMB Enzyme Nomenclature: 3.5.5.5
ExPASy - ENZYME nomenclature database: 3.5.5.5
BRENDA, the Enzyme Database: 3.5.5.5
CAS: 132053-06-6

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