KEGG   ENZYME: 3.6.1.5Help
Entry
EC 3.6.1.5                  Enzyme                                 

Name
apyrase;
ATP-diphosphatase;
adenosine diphosphatase;
ADPase;
ATP diphosphohydrolase [ambiguous]
Class
Hydrolases;
Acting on acid anhydrides;
In phosphorus-containing anhydrides
BRITE hierarchy
Sysname
nucleoside triphosphate phosphohydrolase (nucleoside monophosphoate-forming)
Reaction(IUBMB)
a nucleoside 5'-triphosphate + 2 H2O = a nucleoside 5'-phosphate + 2 phosphate (overall reaction) [RN:R10443];
(1a) a nucleoside 5'-triphosphate + H2O = a nucleoside 5'-diphosphate + phosphate [RN:R02319];
(1b) a nucleoside 5'-diphosphate + H2O = a nucleoside 5'-phosphate + phosphate [RN:R10442]
Reaction(KEGG)
Substrate
nucleoside 5'-triphosphate [CPD:C00201];
H2O [CPD:C00001];
nucleoside 5'-diphosphate [CPD:C00454]
Product
nucleoside 5'-phosphate [CPD:C01117];
phosphate [CPD:C00009];
nucleoside 5'-diphosphate [CPD:C00454]
Comment
Apyrases are active against both di- and triphosphate nucleotides (NDPs and NTPs) and hydrolyse NTPs to nucleotide monophosphates (NMPs) in two distinct successive phosphate-releasing steps, with NDPs as intermediates. They differ from ATPases, which specifically hydrolyse ATP, by hydrolysing both ATP and ADP. The eukaryotic enzymes requires Ca2+, but Mg2+ can substitute. Most of the ecto-ATPases that occur on the cell surface and hydrolyse extracellular nucleotides belong to this enzyme family.
History
EC 3.6.1.5 created 1961, modified 1976, modified 2000, modified 2013
Pathway
ec00230  Purine metabolism
ec00240  Pyrimidine metabolism
Orthology
K01510  apyrase
K14641  apyrase
K14642  golgi apyrase
Genes
HSA: 377841(ENTPD8) 953(ENTPD1) 956(ENTPD3)
PTR: 100614135(ENTPD1) 460286(ENTPD3) 736974(ENTPD8)
PPS: 100971059(ENTPD1) 100983594(ENTPD3) 100995976(ENTPD8)
GGO: 101132716(ENTPD1) 101132747(ENTPD3) 109025377(ENTPD8)
PON: 100442938(ENTPD8) 100447406(ENTPD3) 100453241(ENTPD1)
NLE: 100579317(ENTPD3) 100598542(ENTPD8) 100605921(ENTPD1)
MCC: 703591(ENTPD1) 704424(ENTPD8) 718311(ENTPD3)
MCF: 102132860(ENTPD1) 102134382(ENTPD8) 102138702(ENTPD3)
CSAB: 103216314(ENTPD1) 103227435(ENTPD3) 103239556(ENTPD8)
RRO: 104659869(ENTPD3) 104664681(ENTPD1) 104669616(ENTPD8)
RBB: 108513116(ENTPD3) 108542831(ENTPD8) 108543811(ENTPD1)
CJC: 100386711(ENTPD8) 100399270(ENTPD3) 100404209(ENTPD1)
SBQ: 101027723(ENTPD8) 101037434(ENTPD1) 101044080(ENTPD3)
MMU: 12495(Entpd1) 215446(Entpd3) 72090(Entpd8)
RNO: 103689954 316077(Entpd3) 613267(Entpd8) 64519(Entpd1)
CGE: 100755122(Entpd8) 100762593(Entpd1) 100772668(Entpd3) 107977004
NGI: 103732585(Entpd8) 103733240(Entpd1) 103747123(Entpd3)
HGL: 101696417(Entpd3) 101708535(Entpd8) 101715301(Entpd1)
CCAN: 109687369(Entpd8) 109693654(Entpd1) 109695130(Entpd3)
OCU: 100339644(ENTPD3) 100349881(ENTPD8) 100354213(ENTPD1)
TUP: 102469337(ENTPD3) 102473460(ENTPD1) 102475046(ENTPD8)
CFA: 100688937(ENTPD8) 485604(ENTPD3) 486810(ENTPD1)
AML: 100468369(ENTPD8) 100473727(ENTPD3) 100474255(ENTPD1)
UMR: 103671702(ENTPD1) 103675256(ENTPD8) 103680467(ENTPD3)
ORO: 101362305(ENTPD1) 101366828(ENTPD3) 101377757(ENTPD8)
FCA: 101085582(ENTPD8) 101096356(ENTPD1) 101098083(ENTPD3)
PTG: 102953346(ENTPD8) 102960243(ENTPD1) 102962344(ENTPD3)
AJU: 106966707(ENTPD3) 106984805(ENTPD1) 106989063(ENTPD8)
BTA: 282223(ENTPD1) 506087(ENTPD3) 515532(ENTPD8)
BOM: 102269229(ENTPD8) 102270483(ENTPD1) 102273587(ENTPD3)
BIU: 109553131(ENTPD1) 109576488(ENTPD3)
PHD: 102321352(ENTPD1) 102338946(ENTPD3)
CHX: 102189182(ENTPD1) 102190080(ENTPD3) 108637182(ENTPD8)
OAS: 101107489(ENTPD1) 101114471(ENTPD3) 105612430(ENTPD8)
SSC: 100516719(ENTPD8) 100623360(ENTPD3) 397298(ENTPD1)
CFR: 102503442(ENTPD1) 102509620(ENTPD3) 102520072(ENTPD8)
CDK: 105084264(ENTPD1) 105084627(ENTPD3) 105105103(ENTPD8)
BACU: 103006116(ENTPD8) 103014786(ENTPD1) 103018820(ENTPD3)
LVE: 103069223(ENTPD3) 103078660(ENTPD1) 103080594(ENTPD8)
OOR: 101274730(ENTPD8) 101284487(ENTPD1) 101288931(ENTPD3)
ECB: 100058558(ENTPD8) 100068004(ENTPD3) 100070956(ENTPD1)
EPZ: 103552666(ENTPD3) 103554172(ENTPD8) 103565822(ENTPD1)
EAI: 106822790(ENTPD8) 106842107(ENTPD1) 106842877(ENTPD3)
MYB: 102243699(ENTPD8) 102255350(ENTPD3) 102256383(ENTPD1)
MYD: 102752806(ENTPD1) 102755153(ENTPD8) 102774497(ENTPD3)
HAI: 109380953(ENTPD1) 109381956(ENTPD8) 109393475(ENTPD3)
RSS: 109440844 109443881(ENTPD1) 109445313(ENTPD3) 109460910(ENTPD8)
PALE: 102879249(ENTPD3) 102890548(ENTPD1) 102897950(ENTPD8)
LAV: 100660160(ENTPD8) 100668440(ENTPD3) 100671741(ENTPD1)
MDO: 100022843(ENTPD8) 100022899(ENTPD1) 100023686 100025436(ENTPD3)
SHR: 100914154(ENTPD1) 100919726 100926510(ENTPD3) 100932670
OAA: 100078824(ENTPD3) 100086706
GGA: 374095(ENTPD8) 417281(ENTPD8L) 423826(ENTPD1) 427778 428447(ENTPD3)
APLA: 101795263 101795762(ENTPD8) 101796222 101801191(ENTPD1) 101804288(ENTPD3)
ACYG: 106037034 106037126(ENTPD8) 106037128 106037395(ENTPD1) 106046665(ENTPD3)
TGU: 100218994(ENTPD1) 100221575(ENTPD3) 100226299 100229162(ENTPD8) 105760940
GFR: 102034288(ENTPD3) 102040449(ENTPD8) 102040618 102040786 102043720(ENTPD1)
FAB: 101806865(ENTPD3) 101809481(ENTPD8) 101809685 101817605(ENTPD1) 101818198
PHI: 102099317(ENTPD3) 102105445 102107727(ENTPD8) 102110072(ENTPD1) 102111005
PMAJ: 107200959(ENTPD3) 107206906(ENTPD1) 107212208(ENTPD8) 107212235
CCAE: 111923529(ENTPD3) 111931219(ENTPD1) 111936903(ENTPD8)
CCW: 104688209(ENTPD8) 104688210 104688423 104689029(ENTPD3) 104696030(ENTPD1)
FCH: 102048208 102048554(ENTPD8) 102053733 102054936(ENTPD3) 106630880(ENTPD1)
CLV: 102083509 102083693 102095399(ENTPD1) 102096210(ENTPD8) 102097422(ENTPD3)
EGZ: 104123993 104123994 104124023(ENTPD8) 104127496(ENTPD3) 104129173(ENTPD1)
ASN: 102373368(ENTPD1) 102373540(ENTPD3) 102380815 102381055(ENTPD8) 102381801
AMJ: 102563980(ENTPD8) 102564208 102567238(ENTPD1) 102567497(ENTPD3) 106737136
PSS: 102445405 102445643 102452816(ENTPD1) 102455639(ENTPD3) 102461628(ENTPD8)
CMY: 102934720(ENTPD3) 102946038 102946260 102946489(ENTPD8) 102947751(ENTPD1)
CPIC: 101944035 101946123 101946406(ENTPD8) 101948660(ENTPD1) 101952167(ENTPD3)
ACS: 100553564(entpd1) 100561052(entpd3) 103281931(entpd8)
PVT: 110082161(ENTPD3) 110082944(ENTPD8) 110084053(ENTPD1)
PBI: 103052703(ENTPD3) 103053833(ENTPD1) 103066259(ENTPD8)
GJA: 107111486(ENTPD3) 107120421(ENTPD8) 107121141(ENTPD1)
XLA: 108697462 108698845 108699877(entpd8.S) 443675(entpd3.L) 444164(entpd1.L)
XTR: 100125010(entpd3) 101735145 448501(entpd1) 733458(entpd8)
NPR: 108787246 108787250 108788349(ENTPD1) 108792068(ENTPD3)
DRE: 100005551(entpd3) 436652(entpd8) 445151(entpd1)
IPU: 108268748(entpd3) 108271729(entpd8) 108274253(entpd1)
AMEX: 103029336(entpd1) 103030351(entpd3) 103047612
TRU: 101068571 101070429(entpd3)
LCO: 104924305(entpd1) 104934031(entpd3)
NCC: 104941388(entpd1) 104952129(entpd3)
MZE: 101473382 101487105(entpd1)
OLA: 101166588(entpd1)
XMA: 102217482(entpd1) 102226175(entpd3)
PRET: 103478359 103481559(entpd1)
NFU: 107378765(entpd3) 107387774(entpd1)
KMR: 108232313(entpd3) 108249829(entpd1)
LCF: 108888779(entpd3) 108899023(entpd1)
SDU: 111220166(entpd1) 111227750(entpd3)
HCQ: 109513373(entpd1) 109531055(entpd3)
BPEC: 110171693 110174579(entpd1)
ELS: 105016904 105025925(entpd3) 105026290(entpd1)
SFM: 108920199(entpd8) 108940459(entpd1) 108940642(entpd3)
LCM: 102348651(ENTPD3) 102360392(ENTPD8) 102367063
SKO: 100368830
FCD: 110845793
SHX: MS3_00787
EGL: EGR_01343
ADF: 107336082
HMG: 100199550
LJA: Lj0g3v0157119.2(Lj0g3v0157119.2) Lj0g3v0159839.1(Lj0g3v0159839.1) Lj1g3v3945990.1(Lj1g3v3945990.1) Lj1g3v3945990.2(Lj1g3v3945990.2) Lj1g3v3948060.1(Lj1g3v3948060.1) Lj1g3v3948070.1(Lj1g3v3948070.1) Lj2g3v1277850.1(Lj2g3v1277850.1)
DOSA: Os03t0378000-01(Os03g0378000) Os07t0682800-01(Os07g0682800) Os08t0436100-01(Os08g0436100) Os11t0125900-00(Os11g0125900) Os11t0126800-01(Os11g0126800) Os11t0439600-01(Os11g0439600) Os11t0440200-00(Os11g0440200) Os12t0123500-01(Os12g0123500)
ATS: 109733038(LOC109733038) 109734741(LOC109734741) 109734997(LOC109734997) 109735821(LOC109735821) 109746380(LOC109746380) 109752413(LOC109752413) 109756907(LOC109756907) 109762626(LOC109762626)
APRO: F751_5931
SCE: YER005W(YND1)
ERC: Ecym_4478
KMX: KLMA_50381(YND1)
NCS: NCAS_0I00760(NCAS0I00760)
NDI: NDAI_0A08280(NDAI0A08280)
TPF: TPHA_0L00770(TPHA0L00770)
TBL: TBLA_0A01060(TBLA0A01060)
TDL: TDEL_0H03120(TDEL0H03120)
KAF: KAFR_0L00600(KAFR0L00600)
PIC: PICST_75213(YND1)
CAL: CAALFM_C406230CA(CaO19.2915)
CAUR: QG37_02878
SLB: AWJ20_3098(YND1)
SPO: SPCC11E10.05c(ynd1)
SMIN: v1.2.033068.t1(symbB.v1.2.033068.t1)
LPN: lpg0971
LPH: LPV_1110
LPO: LPO_1060
LPM: LP6_0959
LPF: lpl1000
LPP: lpp1033
LPC: LPC_2316
LPA: lpa_01464
LPE: lp12_0993
 » show all
Taxonomy
Reference
1  [PMID:14953432]
  Authors
KRISHNAN PS.
  Title
Apyrase, pyrophosphatase and metaphosphatase of Penicillium chrysogenum.
  Journal
Arch Biochem 37:224-34 (1952)
DOI:10.1016/0003-9861(52)90180-X
Reference
2  [PMID:13930517]
  Authors
LIEBECQ C, LALLEMAND A, DEGUELDRE-GUILLAUME MJ
  Title
[Partial purification and properties of potato apyrase.]
  Journal
Bull Soc Chim Biol (Paris) 45:573-94 (1963)
Reference
3  [PMID:3038893]
  Authors
Chen YR, Datta N, Roux SJ
  Title
Purification and partial characterization of a calmodulin-stimulated nucleoside triphosphatase from pea nuclei.
  Journal
J Biol Chem 262:10689-94 (1987)
Reference
4  [PMID:8529670]
  Authors
Christoforidis S, Papamarcaki T, Galaris D, Kellner R, Tsolas O
  Title
Purification and properties of human placental ATP diphosphohydrolase.
  Journal
Eur J Biochem 234:66-74 (1995)
DOI:10.1111/j.1432-1033.1995.066_c.x
  Sequence
[hsa:953]
Reference
5  [PMID:8626624]
  Authors
Wang TF, Guidotti G
  Title
CD39 is an ecto-(Ca2+,Mg2+)-apyrase.
  Journal
J Biol Chem 271:9898-901 (1996)
DOI:10.1074/jbc.271.17.9898
  Sequence
[hsa:953]
Reference
6  [PMID:10409709]
  Authors
Gao XD, Kaigorodov V, Jigami Y
  Title
YND1, a homologue of GDA1, encodes membrane-bound apyrase required for Golgi N- and O-glycosylation in Saccharomyces cerevisiae.
  Journal
J Biol Chem 274:21450-6 (1999)
DOI:10.1074/jbc.274.30.21450
  Sequence
[sce:YER005W]
Reference
7  [PMID:15576788]
  Authors
Xu W, Jones CR, Dunn CA, Bessman MJ
  Title
Gene ytkD of Bacillus subtilis encodes an atypical nucleoside triphosphatase member of the Nudix hydrolase superfamily.
  Journal
J Bacteriol 186:8380-4 (2004)
DOI:10.1128/JB.186.24.8380-8384.2004
  Sequence
[bsu:BSU30630]
Other DBs
ExplorEnz - The Enzyme Database: 3.6.1.5
IUBMB Enzyme Nomenclature: 3.6.1.5
ExPASy - ENZYME nomenclature database: 3.6.1.5
BRENDA, the Enzyme Database: 3.6.1.5
CAS: 9000-95-7

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