KEGG   ENZYME: 3.7.1.4Help
Entry
EC 3.7.1.4                  Enzyme                                 

Name
phloretin hydrolase;
ErPhy;
lactase-phlorerin hydrolase;
C-acylphenol hydrolase;
2',4,4',6'-tetrahydroxydehydrochalcone 1,3,5-trihydroxybenzenehydrolase (incorrect)
Class
Hydrolases;
Acting on carbon-carbon bonds;
In ketonic substances
BRITE hierarchy
Sysname
phloretin acylhydrolase (phloroglucinol forming)
Reaction(IUBMB)
phloretin + H2O = phloretate + phloroglucinol [RN:R02901]
Reaction(KEGG)
Substrate
phloretin [CPD:C00774];
H2O [CPD:C00001]
Product
phloretate [CPD:C01744];
phloroglucinol [CPD:C02183]
Comment
Also hydrolyses other C-acylated phenols related to phloretin. Isolated from the fungus Aspergillus niger and the bacteria Pantoea agglomerans and Eubacterium ramulus.
History
EC 3.7.1.4 created 1972, modified 2018
Orthology
K22906  phloretin hydrolase
Genes
RGE: RGE_01480 RGE_12780
TAK: Tharo_0198
LPG: BB562_02250
CLJ: CLJU_c30240
CLS: CXIVA_00870 CXIVA_01400 CXIVA_03650 CXIVA_10480 CXIVA_16390 CXIVA_20190
CSR: Cspa_c22710
CAH: CAETHG_1031
CSQ: CSCA_1095
CBUT: ATN24_18505
FPLA: A4U99_15225
 » show all
Taxonomy
Reference
1  [PMID:5354935]
  Authors
Chatterjee AK, Gibbins LN
  Title
Metabolism of phloridzin by Erwinia herbicola: nature of the degradation products, and the purification and properties of phloretin hydrolase.
  Journal
J Bacteriol 100:594-600 (1969)
Reference
2  [PMID:5441377]
  Authors
Minamikawa T, Jayasankar NP, Bohm BA, Taylor IE, Towers GH.
  Title
An inducible hydrolase from Aspergillus niger, acting on carbon-carbon bonds, for phlorrhizin and other C-acylated phenols.
  Journal
Biochem J 116:889-97 (1970)
Reference
3  [PMID:15466559]
  Authors
Schoefer L, Braune A, Blaut M
  Title
Cloning and expression of a phloretin hydrolase gene from Eubacterium ramulus and characterization of the recombinant enzyme.
  Journal
Appl Environ Microbiol 70:6131-7 (2004)
DOI:10.1128/AEM.70.10.6131-6137.2004
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 3.7.1.4
IUBMB Enzyme Nomenclature: 3.7.1.4
ExPASy - ENZYME nomenclature database: 3.7.1.4
BRENDA, the Enzyme Database: 3.7.1.4
CAS: 37289-38-6

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