KEGG   ENZYME: 4.1.1.83Help
Entry
EC 4.1.1.83                 Enzyme                                 

Name
4-hydroxyphenylacetate decarboxylase;
p-hydroxyphenylacetate decarboxylase;
p-Hpd;
4-Hpd;
4-hydroxyphenylacetate carboxy-lyase
Class
Lyases;
Carbon-carbon lyases;
Carboxy-lyases
BRITE hierarchy
Sysname
4-(hydroxyphenyl)acetate carboxy-lyase (4-methylphenol-forming)
Reaction(IUBMB)
(4-hydroxyphenyl)acetate + H+ = 4-methylphenol + CO2 [RN:R07312]
Reaction(KEGG)
Substrate
4-hydroxyphenylacetate [CPD:C00642];
H+ [CPD:C00080]
Product
4-methylphenol [CPD:C01468];
CO2 [CPD:C00011]
Comment
The enzyme, from the strict anaerobe Clostridium difficile, can also use (3,4-dihydroxyphenyl)acetate as a substrate, yielding 4-methylcatechol as a product. The enzyme is a glycyl radical enzyme.
History
EC 4.1.1.83 created 2005
Orthology
K18427  4-hydroxyphenylacetate decarboxylase large subunit
K18428  4-hydroxyphenylacetate decarboxylase small subunit
Genes
CBK: CLL_A1723 CLL_A1724
CBT: CLH_1871 CLH_1872
CSQ: CSCA_5035 CSCA_5036
CCK: Ccar_15590 Ccar_15595
CDF: CD630_01530 CD630_01540
PDC: CDIF630_00272(hpdB) CDIF630_00273(hpdC)
CDC: CD196_0165(hpdB) CD196_0166(hpdC)
CDL: CDR20291_0152(hpdB) CDR20291_0153(hpdC)
PDF: CD630DERM_01530 CD630DERM_01540
SGY: Sgly_2535 Sgly_2536
 » show all
Taxonomy
Reference
1  [PMID:3938267]
  Authors
D'Ari L, Barker HA.
  Title
p-Cresol formation by cell-free extracts of Clostridium difficile.
  Journal
Arch Microbiol 143:311-2 (1985)
Reference
2  [PMID:11231288]
  Authors
Selmer T, Andrei PI.
  Title
p-Hydroxyphenylacetate decarboxylase from Clostridium difficile. A novel glycyl radical enzyme catalysing the formation of p-cresol.
  Journal
Eur J Biochem 268:1363-72 (2001)
DOI:10.1046/j.1432-1327.2001.02001.x
  Sequence
Reference
3  [PMID:15153112]
  Authors
Andrei PI, Pierik AJ, Zauner S, Andrei-Selmer LC, Selmer T.
  Title
Subunit composition of the glycyl radical enzyme p-hydroxyphenylacetate decarboxylase. A small subunit, HpdC, is essential for catalytic activity.
  Journal
Eur J Biochem 271:2225-30 (2004)
DOI:10.1111/j.1432-1033.2004.04152.x
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 4.1.1.83
IUBMB Enzyme Nomenclature: 4.1.1.83
ExPASy - ENZYME nomenclature database: 4.1.1.83
BRENDA, the Enzyme Database: 4.1.1.83
CAS: 340137-18-0

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