KEGG   ENZYME: 4.1.2.45Help
Entry
EC 4.1.2.45                 Enzyme                                 

Name
trans-o-hydroxybenzylidenepyruvate hydratase-aldolase;
2'-hydroxybenzalpyruvate aldolase;
NsaE;
tHBPA hydratase-aldolase
Class
Lyases;
Carbon-carbon lyases;
Aldehyde-lyases
BRITE hierarchy
Sysname
(3E)-4-(2-hydroxyphenyl)-2-oxobut-3-enoate hydro-lyase
Reaction(IUBMB)
(3E)-4-(2-hydroxyphenyl)-2-oxobut-3-enoate + H2O = salicylaldehyde + pyruvate [RN:R05136]
Reaction(KEGG)
Substrate
(3E)-4-(2-hydroxyphenyl)-2-oxobut-3-enoate [CPD:C06203];
H2O [CPD:C00001]
Product
salicylaldehyde [CPD:C06202];
pyruvate [CPD:C00022]
Comment
This enzyme is involved in naphthalene degradation. The enzyme catalyses a retro-aldol reaction in vitro, and it accepts a broad range of aldehydes and 4-substituted 2-oxobut-3-enoates as substrates [4].
History
EC 4.1.2.45 created 2010, modified 2011
Pathway
ec00626  Naphthalene degradation
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K14585  trans-o-hydroxybenzylidenepyruvate hydratase-aldolase
Genes
PPI: ND061(nahE)
PSC: A458_06700
PSTU: UIB01_22650
PBM: CL52_14635
PFK: PFAS1_28640
ALT: ambt_17910
CYQ: Q91_1157
CZA: CYCME_1270
CYY: CPC19_06820
GBI: PG2T_00740 PG2T_05760 PG2T_12860
BMK: DM80_1449(phdJ)
BFN: OI25_3035 OI25_3053(phdJ)
PNA: Pnap_2479
CID: P73_0351
NAR: Saro_3836
 » show all
Taxonomy
Reference
1  [PMID:8486638]
  Authors
Kuhm AE, Knackmuss HJ, Stolz A
  Title
Purification and properties of 2'-hydroxybenzalpyruvate aldolase from a bacterium that degrades naphthalenesulfonates.
  Journal
J Biol Chem 268:9484-9 (1993)
Reference
2  [PMID:16804169]
  Authors
Keck A, Conradt D, Mahler A, Stolz A, Mattes R, Klein J
  Title
Identification and functional analysis of the genes for naphthalenesulfonate catabolism by Sphingomonas xenophaga BN6.
  Journal
Microbiology 152:1929-40 (2006)
DOI:10.1099/mic.0.28783-0
  Sequence
Reference
3  [PMID:8002605]
  Authors
Eaton RW
  Title
Organization and evolution of naphthalene catabolic pathways: sequence of the DNA encoding 2-hydroxychromene-2-carboxylate isomerase and trans-o-hydroxybenzylidenepyruvate hydratase-aldolase from the NAH7 plasmid.
  Journal
J Bacteriol 176:7757-62 (1994)
DOI:10.1128/JB.176.24.7757-7762.1994
  Sequence
Reference
4  [PMID:10831455]
  Authors
Eaton RW
  Title
trans-o-Hydroxybenzylidenepyruvate hydratase-aldolase as a biocatalyst.
  Journal
Appl Environ Microbiol 66:2668-72 (2000)
DOI:10.1128/AEM.66.6.2668-2672.2000
Other DBs
ExplorEnz - The Enzyme Database: 4.1.2.45
IUBMB Enzyme Nomenclature: 4.1.2.45
ExPASy - ENZYME nomenclature database: 4.1.2.45
BRENDA, the Enzyme Database: 4.1.2.45

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