KEGG   ENZYME: 4.1.3.24Help
Entry
EC 4.1.3.24                 Enzyme                                 

Name
malyl-CoA lyase;
malyl-coenzyme A lyase;
(3S)-3-carboxy-3-hydroxypropanoyl-CoA glyoxylate-lyase;
mclA (gene name);
mcl1 (gene name);
(3S)-3-carboxy-3-hydroxypropanoyl-CoA glyoxylate-lyase (acetyl-CoA-forming);
L-malyl-CoA lyase
Class
Lyases;
Carbon-carbon lyases;
Oxo-acid-lyases
BRITE hierarchy
Sysname
(S)-malyl-CoA glyoxylate-lyase (acetyl-CoA-forming)
Reaction(IUBMB)
(1) (S)-malyl-CoA = acetyl-CoA + glyoxylate [RN:R00473];
(2) (2R,3S)-2-methylmalyl-CoA = propanoyl-CoA + glyoxylate [RN:R00934]
Reaction(KEGG)
Substrate
(S)-malyl-CoA [CPD:C04348];
(2R,3S)-2-methylmalyl-CoA [CPD:C06027]
Product
acetyl-CoA [CPD:C00024];
glyoxylate [CPD:C00048];
propanoyl-CoA [CPD:C00100]
Comment
The enzymes from Rhodobacter species catalyse a step in the ethylmalonyl-CoA pathway for acetate assimilation [3,5]. The enzyme from halophilic bacteria participate in the methylaspartate cycle and catalyse the reaction in the direction of malyl-CoA formation [6]. The enzyme from the bacterium Chloroflexus aurantiacus, which participates in the 3-hydroxypropanoate cycle for carbon assimilation, also has the activity of EC 4.1.3.25, (3S)-citramalyl-CoA lyase [2,4].
History
EC 4.1.3.24 created 1972, modified 2014
Pathway
ec00630  Glyoxylate and dicarboxylate metabolism
ec00660  C5-Branched dibasic acid metabolism
ec00680  Methane metabolism
ec00720  Carbon fixation pathways in prokaryotes
ec01120  Microbial metabolism in diverse environments
Orthology
K08691  malyl-CoA/(S)-citramalyl-CoA lyase
K19281  beta-methylmalyl-CoA/(S)-malyl-CoA lyase
K19282  bifunctional (S)-malyl-CoA lyase/thioesterase
Genes
MCA: MCA1739
BLEP: AL038_08990
RPJ: N234_12055
CNC: CNE_BB1p10140(mcl)
BGP: BGL_2c16890
BPLA: bpln_2g17560
BPH: Bphy_5924
BFN: OI25_5771 OI25_7353
BCAI: K788_0001579
PHS: C2L64_21705
CBAB: SMCB_1586
MPT: Mpe_A3254
METR: BSY238_253
PLA: Plav_3098
RBS: RHODOSMS8_01011(mcl)
BRAD: BF49_2901
XAU: Xaut_3870
AZC: AZC_1227
MDI: METDI2485(mcl) METDI4906
MAQU: Maq22A_c18385(citE) Maq22A_c19250(mcl)
MSL: Msil_1719
HDN: Hden_1659
RVA: Rvan_1682
BVR: BVIR_1803
MCG: GL4_3371
HDI: HDIA_2467(mcl1_2)
RBM: TEF_05695
CCR: CC_2999
CAK: Caul_0731
CSE: Cseg_3490
SIL: SPO1565 SPO3608(mclA)
RSP: RSP_1771
RCP: RCAP_rcc03164(citE2)
JAN: Jann_0471
RDE: RD1_1001(citE) RD1_1160(mclA) RD1_4242(mclA)
RLI: RLO149_c002070(mcl1) RLO149_c036850(mcl2)
PDE: Pden_0799
DSH: Dshi_3050(citE2)
PSF: PSE_3192
PGD: Gal_00369
OTM: OSB_28710(mcl1)
PTP: RCA23_c05980(mclA)
CID: P73_4243
MALG: MALG_03008
RSU: NHU_02809
RHC: RGUI_3145
SPSE: SULPSESMR1_03496(mcl1)
RMM: ROSMUCSMR3_02149(mcl1)
LVS: LOKVESSMR4R_02838(mcl1)
AHT: ANTHELSMS3_00280(mcl1)
HNE: HNE_0078(mclA)
HBA: Hbal_0778
RRU: Rru_A0217
RRF: F11_01090
MAG: amb3977
MAGX: XM1_1373
MAGN: WV31_14965
TMO: TMO_0350(citE)
TXI: TH3_02680
MAGQ: MGMAQ_0659
SCT: SCAT_4364
SALU: DC74_4078
SALL: SAZ_21750
STRE: GZL_04819
SLD: T261_4311
NCA: Noca_4393
NDK: I601_0974(mcl1)
PSIM: KR76_23920
AMD: AMED_0079(citE)
AMN: RAM_00395
AMM: AMES_0076(citE)
AMZ: B737_0077(citE)
AOI: AORI_0068(mcl)
AMQ: AMETH_0090(citE)
KAL: KALB_2473
CWO: Cwoe_4213
RCA: Rcas_0912
CAU: Caur_0174
CAG: Cagg_1328
HMA: rrnAC0690(citE1) rrnAC1965(aceB)
HHI: HAH_1341(citE1) HAH_2476(aceB)
NPH: NP_4430A(aceB)
NMO: Nmlp_2357(aceB)
HMU: Hmuk_2984
HWA: HQ_1720A(aceB)
HWC: Hqrw_1842(aceB)
HVO: HVO_1983(aceB1) HVO_B0200(aceB2)
HME: HFX_2078(aceB)
NMG: Nmag_3338(mcl) Nmag_3363(aceB)
NAT: NJ7G_3289
SALI: L593_04660
 » show all
Taxonomy
Reference
1  [PMID:14285256]
  Authors
TUBOI S, KIKUCHI G.
  Title
ENZYMIC CLEAVAGE OF MALYTL-COENZYME A INTO ACETYL-COENZYME A AND GLYOXYLIC ACID.
  Journal
Biochim Biophys Acta 96:148-53 (1965)
DOI:10.1016/0005-2787(65)90618-0
Reference
2  [PMID:12374834]
  Authors
Herter S, Busch A, Fuchs G.
  Title
L-Malyl-coenzyme A lyase/beta-methylmalyl-coenzyme A lyase from Chloroflexus aurantiacus, a bifunctional enzyme involved in autotrophic CO(2) fixation.
  Journal
J Bacteriol 184:5999-6006 (2002)
DOI:10.1128/JB.184.21.5999-6006.2002
  Sequence
[cau:Caur_0174]
Reference
3  [PMID:15687206]
  Authors
Meister M, Saum S, Alber BE, Fuchs G
  Title
L-malyl-coenzyme A/beta-methylmalyl-coenzyme A lyase is involved in acetate assimilation of the isocitrate lyase-negative bacterium Rhodobacter capsulatus.
  Journal
J Bacteriol 187:1415-25 (2005)
DOI:10.1128/JB.187.4.1415-1425.2005
  Sequence
Reference
4  [PMID:17259315]
  Authors
Friedmann S, Alber BE, Fuchs G
  Title
Properties of R-citramalyl-coenzyme A lyase and its role in the autotrophic 3-hydroxypropionate cycle of Chloroflexus aurantiacus.
  Journal
J Bacteriol 189:2906-14 (2007)
DOI:10.1128/JB.01620-06
Reference
5  [PMID:20047909]
  Authors
Erb TJ, Frerichs-Revermann L, Fuchs G, Alber BE
  Title
The apparent malate synthase activity of Rhodobacter sphaeroides is due to two paralogous enzymes, (3S)-Malyl-coenzyme A (CoA)/{beta}-methylmalyl-CoA lyase and  (3S)- Malyl-CoA thioesterase.
  Journal
J Bacteriol 192:1249-58 (2010)
DOI:10.1128/JB.01267-09
  Sequence
[rsp:RSP_1771]
Reference
6  [PMID:27920298]
  Authors
Borjian F, Han J, Hou J, Xiang H, Zarzycki J, Berg IA
  Title
Malate Synthase and beta-Methylmalyl Coenzyme A Lyase Reactions in the Methylaspartate Cycle in Haloarcula hispanica.
  Journal
J Bacteriol 199 (2017)
DOI:10.1128/JB.00657-16
Other DBs
ExplorEnz - The Enzyme Database: 4.1.3.24
IUBMB Enzyme Nomenclature: 4.1.3.24
ExPASy - ENZYME nomenclature database: 4.1.3.24
BRENDA, the Enzyme Database: 4.1.3.24
CAS: 37290-67-8

DBGET integrated database retrieval system