KEGG   ENZYME: 4.1.3.39Help
Entry
EC 4.1.3.39                 Enzyme                                 

Name
4-hydroxy-2-oxovalerate aldolase;
4-hydroxy-2-ketovalerate aldolase;
HOA;
DmpG;
4-hydroxy-2-oxovalerate pyruvate-lyase;
4-hydroxy-2-oxopentanoate pyruvate-lyase;
BphI;
4-hydroxy-2-oxopentanoate pyruvate-lyase (acetaldehyde-forming)
Class
Lyases;
Carbon-carbon lyases;
Oxo-acid-lyases
BRITE hierarchy
Sysname
(S)-4-hydroxy-2-oxopentanoate pyruvate-lyase (acetaldehyde-forming)
Reaction(IUBMB)
(S)-4-hydroxy-2-oxopentanoate = acetaldehyde + pyruvate
Reaction(KEGG)
(other) R00750
Show
Substrate
(S)-4-hydroxy-2-oxopentanoate
Product
acetaldehyde [CPD:C00084];
pyruvate [CPD:C00022]
Comment
Requires Mn2+ for maximal activity [1]. The enzyme from the bacterium Pseudomonas putida is also stimulated by NADH [1]. In some bacterial species the enzyme forms a bifunctional complex with EC 1.2.1.10, acetaldehyde dehydrogenase (acetylating). The enzymes from the bacteria Burkholderia xenovorans and Thermus thermophilus also perform the reaction of EC 4.1.3.43, 4-hydroxy-2-oxohexanoate aldolase [4,5].
History
EC 4.1.3.39 created 2006, modified 2011
Pathway
ec00360  Phenylalanine metabolism
ec00362  Benzoate degradation
ec00621  Dioxin degradation
ec00622  Xylene degradation
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K01666  4-hydroxy 2-oxovalerate aldolase
K18365  4-hydroxy-2-oxovalerate/4-hydroxy-2-oxohexanoate aldolase
Genes
ECO: b0352(mhpE)
ECJ: JW0343(mhpE)
EBW: BWG_0241(mhpE)
ECOK: ECMDS42_0274(mhpE)
ECE: Z0452(mhpE)
ECS: ECs0407
ECF: ECH74115_0427(mhpE)
ETW: ECSP_0416(mhpE)
ELX: CDCO157_0395
EOJ: ECO26_0388(mhpE)
EOI: ECO111_0388(mhpE)
EOH: ECO103_0334(mhpE)
ECOO: ECRM13514_0494(mhpE)
ECOH: ECRM13516_0332(mhpE)
EOK: G2583_0465(mhpE)
ESO: O3O_05580
ESM: O3M_19700
ESL: O3K_19715
ECW: EcE24377A_0376(mhpE)
ELH: ETEC_0408
EUN: UMNK88_402(mhpE)
ECX: EcHS_A0416(mhpE)
ECM: EcSMS35_0383(mhpE)
ECY: ECSE_0377
ECR: ECIAI1_0353(mhpE)
ECQ: ECED1_0380(mhpE)
ECK: EC55989_0359(mhpE)
ECT: ECIAI39_0326(mhpE)
EOC: CE10_0320(mhpE)
EUM: ECUMN_0395(mhpE)
ELO: EC042_0389(mhpE)
ESE: ECSF_0323
EBR: ECB_00306(mhpE)
EBD: ECBD_3305
EKF: KO11_21820(mhpE)
EDJ: ECDH1ME8569_0339(mhpE)
ENA: ECNA114_0335(mhpE)
ELW: ECW_m0430(mhpE)
ELL: WFL_02115(mhpE)
ELP: P12B_c0312(mhpE) P12B_c0370(mhpE)
EBL: ECD_00306(mhpE)
EBE: B21_00310(mhpE)
ECOJ: P423_01850
ECOS: EC958_0496
EMA: C1192_10895(dmpG)
SSN: SSON_0331(mhpE)
KPN: KPN_02117(mhpE)
KPU: KP1_3212(mhpE)
KPP: A79E_2127
KPT: VK055_0344(dmpG)
KPE: KPK_2206(mhpE)
KPR: KPR_2662(mhpE)
KPJ: N559_2173
KPX: PMK1_04481(mhpE)
KPNU: LI86_10795
KPNK: BN49_3222(mhpE)
KVA: Kvar_2158
KOX: KOX_22670
KOE: A225_3411
KQV: B8P98_11815(dmpG)
CWE: CO701_16970(dmpG)
CYO: CD187_18570(dmpG)
CIF: AL515_06460(dmpG)
CIE: AN232_24605(dmpG)
EBT: EBL_c19990(mhpE)
RAO: DSD31_10730(dmpG)
EBF: D782_1987
SPE: Spro_3025
SERA: Ser39006_017380(dmpG)
ECA: ECA1429(nahM)
PATR: EV46_07235
PATO: GZ59_14680(nahM)
EPY: EpC_04020
PLU: plu4081
XNE: XNC1_0240
XNM: XNC2_0241
LRI: NCTC12151_03194(mhpE)
PSD: DSC_09375
VAG: N646_2394
PAEP: PA1S_20450
PAEG: AI22_13500
PCQ: PcP3B5_23500(mhpE) PcP3B5_25720(dmpG)
PPU: PP_1791
PPT: PPS_1433
PPI: ND084(nahM)
PPX: T1E_4258(cmtG) T1E_4274(tobH)
PPUH: B479_25865
PPUN: PP4_27280(mhpE)
PSB: Psyr_3072
PEN: PSEEN0975
PSTT: CH92_19040
PPUU: PputUW4_01662(mhpE)
PKC: PKB_1193 PKB_3256(amng1) PKB_3604(amng3) PKB_4237(nahM)
PSES: PSCI_2058(xylK)
PSOS: POS17_2261
AVN: Avin_08740(xylK) Avin_15130(mphE) Avin_30570(lapG) Avin_42120
AVL: AvCA_08740(xylK) AvCA_15130(mphE) AvCA_30570(lapG) AvCA_42120
ACX: Achr_27750(mphE) Achr_32700(xylK)
ASJ: AsACE_CH00352(nahM)
ADV: DJ533_01780(dmpG) DJ533_16960(dmpG)
ARJ: DOM24_01575(dmpG)
SDN: Sden_2668
SPL: Spea_2116
SHL: Shal_2088
SWD: Swoo_1390
PHA: PSHAa2143(mhpE)
PTN: PTRA_a0482(mhpE) PTRA_a2596(bphI)
PNG: PNIG_a2790(bphI)
MHC: MARHY0649
SALH: HMF8227_02732(bphI)
TTU: TERTU_1283(dmpG)
LPN: lpg2681
LPH: LPV_3027
LPO: LPO_2962
LPM: LP6_2713
LPF: lpl2608
LPP: lpp2735
LPC: LPC_0455
LPA: lpa_03915
LPE: lp12_2674
LLO: LLO_0366
LFA: LFA_0384
LHA: LHA_2302
LLG: 44548918_00735(bphI)
TMC: LMI_2790
MEJ: Q7A_1101
CYQ: Q91_0510 Q91_1491(lapG)
CZA: CYCME_0969(lapG) CYCME_2101(dmpG)
CYY: CPC19_05395(dmpG) CPC19_10505(dmpG)
AEH: Mlg_2462
KAK: Kalk_07865(dmpG)
SOK: D0B54_04235(dmpG) D0B54_06190(dmpG)
TBN: TBH_C0930
GPB: HDN1F_27070(tesG)
CHRO: CXB49_03860(dmpG)
RSO: RSp0895(dmpG)
RSM: CMR15_mp10879(mhpE)
RSE: F504_4322
RPI: Rpic_4617
REH: H16_A1807(mhpE2) H16_B0552(mhpE1) H16_B0595(bpHI)
RME: Rmet_1319(bphI) Rmet_5209(mhpE)
CTI: RALTA_B0399(mhpE)
BPS: BPSS1807(mhpE)
BPM: BURPS1710b_A0890(mhpE)
BPD: BURPS668_A2590(mhpE)
BPSE: BDL_5207(dmpG)
BPSM: BBQ_4325(dmpG)
BPSU: BBN_5270(dmpG)
BPSD: BBX_4373(dmpG)
BPZ: BP1026B_II1937(mhpE)
BPK: BBK_4537(dmpG)
BPSH: DR55_5932(dmpG)
BPSA: BBU_4207(dmpG)
BPSO: X996_5729(dmpG)
BUT: X994_5292(dmpG)
BTE: BTH_II0570(mhpE)
BTQ: BTQ_3864(dmpG)
BTJ: BTJ_4896(dmpG)
BTZ: BTL_5690(dmpG)
BTD: BTI_5186(dmpG)
BTV: BTHA_4520(dmpG)
BTHE: BTN_5352(dmpG)
BTHM: BTRA_5626(dmpG)
BTHA: DR62_5490
BTHL: BG87_5697(dmpG)
BOK: DM82_6079(dmpG)
BOC: BG90_4586(dmpG)
BVE: AK36_3502(dmpG)
BCJ: BCAM1615(bphI) BCAM2121
BCEN: DM39_1991(dmpG) DM39_3381(dmpG) DM39_4832(dmpG) DM39_4966(dmpG)
BCEW: DM40_5000(dmpG)
BCEO: I35_5475(bphI) I35_6003
BMUL: NP80_3434(dmpG) NP80_3873(dmpG)
BCED: DM42_4991(dmpG) DM42_6343(dmpG)
BLAT: WK25_27480
BSEM: WJ12_26060
BSTG: WT74_26425
BGU: KS03_3374(dmpG) KS03_5342
BGO: BM43_7072(dmpG)
BUK: MYA_5002
BUL: BW21_2671(dmpG) BW21_4722(dmpG)
BXB: DR64_1245(dmpG) DR64_3311(dmpG) DR64_4657(dmpG) DR64_8618(dmpG)
BFN: OI25_255(dmpG) OI25_4591(dmpG)
PARB: CJU94_31730(dmpG) CJU94_37880(dmpG) CJU94_38125(dmpG)
PHS: C2L64_35640(dmpG)
PTER: C2L65_38855(dmpG)
PGP: CUJ91_31740(dmpG)
PPNM: LV28_21850
PPUL: RO07_16240
PSPU: NA29_20920
PAPI: SG18_00060
BAV: BAV0600(bphF)
BHZ: ACR54_00829(mhpE)
AXY: AXYL_04307(dmpG)
ACHR: C2U31_03430(dmpG)
PUT: PT7_2460
AFQ: AFA_13960(dmpG)
RFR: Rfer_0454
POL: Bpro_5136
PNA: Pnap_4142
AJS: Ajs_0224
ACK: C380_15915(bphF)
VEI: Veis_2781
VBO: CKY39_22055(dmpG) CKY39_28495(dmpG)
SIMP: C6571_13625(dmpG) C6571_19090(dmpG)
MELA: C6568_07850(dmpG)
JAG: GJA_232(dmpG)
HEE: hmeg3_19565(dmpG)
LCH: Lcho_3340
DEY: HYN24_14110(dmpG) HYN24_14200(dmpG)
AZO: azo1857(lapG) azo1972(mhpE) azo2431(nahM)
ACOM: CEW83_03610(dmpG)
THK: CCZ27_04605(dmpG) CCZ27_10750(dmpG)
CCF: YSQ_01790
ABU: Abu_1489
ABT: ABED_1386
SHAL: SHALO_2223
SUN: SUN_2142
GLO: Glov_0787
DMA: DMR_24610(nahM)
DPI: BN4_11362(nahM)
HOH: Hoch_1083
RBT: NOVO_02090(leuA)
ARA: Arad_7255
SHZ: shn_33310
BTR: BT_2080
XAU: Xaut_0938
MSL: Msil_1479
MMED: Mame_00137(mhpE)
RUE: DT065_12685(dmpG) DT065_13790(dmpG) DT065_15115(dmpG) DT065_15205(dmpG)
PHP: PhaeoP97_04061(leuA_2)
PPIC: PhaeoP14_03833(leuA_2)
CID: P73_1382
CEH: CEW89_11445(dmpG)
NOV: TQ38_027305(dmpG)
SGI: SGRAN_1422(mhpE2) SGRAN_1579(mhpE)
SPHO: C3E99_03975(dmpG)
SPHU: SPPYR_1439(mhpE)
SPHM: G432_01975
SJP: SJA_C1-11910(mhpE_xylK)
SPHB: EP837_03396(mhpE)
SINB: SIDU_08310
SHYD: CJD35_06515(dmpG)
SFLA: SPHFLASMR4Y_02366(bphI)
SPHY: CHN51_06975(dmpG) CHN51_18315(dmpG)
RDI: CMV14_04950(dmpG)
ERK: CD351_08255(dmpG)
PHZ: CHX26_05330(dmpG)
TMO: TMO_3169(mhpE) TMO_b0123
TXI: TH3_21418
MAGQ: MGMAQ_0651
BSS: BSUW23_00995(rhiG)
BCA: BCE_2157
BCX: BCA_2155(dmpG)
BCF: bcf_10215
BCER: BCK_24220
BTL: BALH_1844
BMYC: DJ92_4638(dmpG)
BMQ: BMQ_1728 BMQ_3587(dmpG)
BMD: BMD_1715 BMD_3572(dmpG)
BMEG: BG04_4046 BG04_461(dmpG)
BACO: OXB_2503
BEO: BEH_08655
BKO: CKF48_08550(dmpG)
GTN: GTNG_3151(nbaI)
GGH: GHH_c14840(pheE)
LYB: C3943_22675(dmpG)
LYZ: DCE79_01755(dmpG)
PMW: B2K_02290
PRI: PRIO_1606
PSWU: SY83_12875
ASOC: CB4_02452
AAC: Aaci_1619
AAD: TC41_1515(mhpE)
BTS: Btus_0969
KYR: CVV65_11290(dmpG)
SIV: SSIL_0066(nbaI) SSIL_3690
SOB: CSE16_14630(dmpG)
KUR: ASO14_173
ESR: ES1_22750
ESU: EUS_17010
COO: CCU_13650
CSO: CLS_02690
DSY: DSY3306
DHD: Dhaf_1245
PTH: PTH_0483(LeuA)
TMR: Tmar_1781
SAY: TPY_0642(mhpE)
BPRS: CK3_33700
CHY: CHY_1280(mhpE)
MTA: Moth_1775
TOC: Toce_0378
LPIL: LIP_2173
MTU: Rv3469c(mhpE) Rv3534c(hsaF)
MTC: MT3575(bphI-1) MT3638(bphI-2)
MRA: MRA_3509(mhpE) MRA_3573(bphI)
MTD: UDA_3469c(mhpE) UDA_3534c
MBO: BQ2027_MB3498C(mhpE) BQ2027_MB3564C(hsaf)
MBB: BCG_3534c(mhpE) BCG_3598c
MBT: JTY_3534(mhpE) JTY_3599
MAF: MAF_34840(mhpE) MAF_35460
MPA: MAP_0533
MAO: MAP4_3334
MAVI: RC58_16535
MAVU: RE97_16570
MAV: MAV_0627
MID: MIP_00991
MYO: OEM_05440
MIR: OCQ_05520
MMAL: CKJ54_02610(dmpG)
MLP: MLM_0772
MMM: W7S_02630
MSG: MSMEI_4052 MSMEI_5778(bphI-2)
MGI: Mflv_1522
MVQ: MYVA_5129
MTHN: 4412656_04050(bphI)
MABB: MASS_0596
MCHE: BB28_03000
MSTE: MSTE_00585
MTER: 4434518_03651(bphI)
CDR: CDHC03_1949(mhpE)
CAMG: CAMM_02595
NFR: ERS450000_00749(dmpG_1) ERS450000_01014(dmpG_2) ERS450000_03770(mhpE)
NCY: NOCYR_0495(mhpE)
NSR: NS506_01252(mhpE)
REQ: REQ_42060
RHS: A3Q41_02907(mhpE)
RRZ: CS378_11835(dmpG) CS378_19560(dmpG) CS378_23185(dmpG) CS378_24200(dmpG)
RQI: C1M55_04085(dmpG) C1M55_29425(dmpG)
RRT: 4535765_04741(mhpE)
GPO: GPOL_c41170(hsaF)
GOR: KTR9_0812(hsaF)
GOC: CXX93_11335(dmpG)
GIT: C6V83_12290(dmpG)
TPR: Tpau_3800
DIT: C3V38_09240(dmpG)
DIZ: CT688_01915(dmpG) CT688_03155(dmpG)
SGR: SGR_564
SCB: SCAB_15191 SCAB_2071(mhpE)
SALU: DC74_7440
SALL: SAZ_38405
SLD: T261_8059
STRM: M444_14905
SLE: sle_57500(sle_57500)
KSK: KSE_23710
SALC: C2138_13080(dmpG)
NDK: I601_0557(mhpE) I601_3471
AEZ: C3E78_16535(dmpG)
NDA: Ndas_2258
SRO: Sros_9113
FAL: FRAAL4663
NML: Namu_4081
AMD: AMED_4220(mhpE)
AMN: RAM_21495
AMM: AMES_4170(mhpE)
AMZ: B737_4170(mhpE)
AOI: AORI_3691(mhpE)
AMQ: AMETH_2976(mhpE)
AMYC: CU254_20390(dmpG)
PDX: Psed_4179
MIL: ML5_3357
AFS: AFR_23745
PLK: CIK06_08080(dmpG) CIK06_17415(dmpG)
SYW: SYNW0424
RCA: Rcas_2410
CAU: Caur_1351
CAG: Cagg_2034
TRO: trd_1392
TTJ: TTHB246
TSC: TSC_c11570(dmpG)
TTF: THTE_4184
TPI: TREPR_1324(dmpG)
BRM: Bmur_0128
BIP: Bint_1446
FSC: FSU_2557
GFL: GRFL_0092
MARM: YQ22_08335
NDO: DDD_3546
CTS: Ctha_2137
MARC: AR505_0545
MLA: Mlab_0987
NMR: Nmar_0140
 » show all
Taxonomy
Reference
1  [PMID:12764229]
  Authors
Manjasetty BA, Powlowski J, Vrielink A.
  Title
Crystal structure of a bifunctional aldolase-dehydrogenase: sequestering a reactive and volatile intermediate.
  Journal
Proc Natl Acad Sci U S A 100:6992-7 (2003)
DOI:10.1073/pnas.1236794100
  Sequence
Reference
2  [PMID:8419288]
  Authors
Powlowski J, Sahlman L, Shingler V.
  Title
Purification and properties of the physically associated meta-cleavage pathway enzymes 4-hydroxy-2-ketovalerate aldolase and aldehyde dehydrogenase (acylating) from Pseudomonas sp. strain CF600.
  Journal
J Bacteriol 175:377-85 (1993)
DOI:10.1128/JB.175.2.377-385.1993
  Sequence
Reference
3  [PMID:11264589]
  Authors
Manjasetty BA, Croteau N, Powlowski J, Vrielink A.
  Title
Crystallization and preliminary X-ray analysis of dmpFG-encoded 4-hydroxy-2-ketovalerate aldolase--aldehyde dehydrogenase (acylating) from Pseudomonas sp. strain CF600.
  Journal
Acta Crystallogr D Biol Crystallogr 57:582-5 (2001)
Reference
4  [PMID:21425833]
  Authors
Baker P, Carere J, Seah SY
  Title
Probing the molecular basis of substrate specificity, stereospecificity, and catalysis in the class II pyruvate aldolase, BphI.
  Journal
Biochemistry 50:3559-69 (2011)
DOI:10.1021/bi101947g
  Sequence
[bxe:Bxe_C1187]
Reference
5  [PMID:22316175]
  Authors
Baker P, Hillis C, Carere J, Seah SY
  Title
Protein-protein interactions and substrate channeling in orthologous and chimeric aldolase-dehydrogenase complexes.
  Journal
Biochemistry 51:1942-52 (2012)
DOI:10.1021/bi201832a
  Sequence
[ttj:TTHB246]
Reference
6  [PMID:22081904]
  Authors
Baker P, Seah SY
  Title
Rational design of stereoselectivity in the class II pyruvate aldolase BphI.
  Journal
J Am Chem Soc 134:507-13 (2012)
DOI:10.1021/ja208754r
  Sequence
[bxe:Bxe_C1187]
Other DBs
ExplorEnz - The Enzyme Database: 4.1.3.39
IUBMB Enzyme Nomenclature: 4.1.3.39
ExPASy - ENZYME nomenclature database: 4.1.3.39
UM-BBD (Biocatalysis/Biodegradation Database): 4.1.3.39
BRENDA, the Enzyme Database: 4.1.3.39
CAS: 37325-52-3

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