KEGG   ENZYME: 4.1.99.11Help
Entry
EC 4.1.99.11                Enzyme                                 

Name
benzylsuccinate synthase;
benzylsuccinate fumarate-lyase
Class
Lyases;
Carbon-carbon lyases;
Other carbon-carbon lyases
BRITE hierarchy
Sysname
benzylsuccinate fumarate-lyase (toluene-forming)
Reaction(IUBMB)
benzylsuccinate = toluene + fumarate [RN:R05598]
Reaction(KEGG)
Substrate
benzylsuccinate [CPD:C09816]
Product
toluene [CPD:C01455];
fumarate [CPD:C00122]
Comment
A glycyl radical enzyme that is inhibited by benzyl alcohol, benzaldehyde, phenylhydrazine and is inactivated by oxygen.
History
EC 4.1.99.11 created 2000
Pathway
ec00623  Toluene degradation
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K07540  benzylsuccinate synthase
Genes
EBA: c2A303(bssC) c2A304(bssA) c2A305(bssB)
AZI: AzCIB_4504 AzCIB_4505 AzCIB_4506
GME: Gmet_1538(bssB) Gmet_1539(bssA) Gmet_1540(bssC)
GEO: Geob_2437(bssB-2) Geob_2438(bssA-2) Geob_2439(bssC-2) Geob_2447(bssB-1) Geob_2448(bssA-1) Geob_2449(bssC-1)
DAL: Dalk_2198
DTO: TOL2_C01390(bssB) TOL2_C01400(bssA) TOL2_C01410(bssC)
DGI: Desgi_0659 Desgi_0660
Taxonomy
Reference
1  [PMID:9765580]
  Authors
Beller HR, Spormann AM.
  Title
Analysis of the novel benzylsuccinate synthase reaction for anaerobic toluene activation based on structural studies of the product.
  Journal
J Bacteriol 180:5454-7 (1998)
Reference
2  [PMID:9632263]
  Authors
Leuthner B, Leutwein C, Schulz H, Horth P, Haehnel W, Schiltz E, Schagger H, Heider J.
  Title
Biochemical and genetic characterization of benzylsuccinate synthase from Thauera aromatica: a new glycyl radical enzyme catalysing the first step in anaerobic toluene metabolism.
  Journal
Mol Microbiol 28:615-28 (1998)
DOI:10.1046/j.1365-2958.1998.00826.x
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 4.1.99.11
IUBMB Enzyme Nomenclature: 4.1.99.11
ExPASy - ENZYME nomenclature database: 4.1.99.11
UM-BBD (Biocatalysis/Biodegradation Database): 4.1.99.11
BRENDA, the Enzyme Database: 4.1.99.11
CAS: 209264-18-6

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