KEGG   ENZYME: 4.1.99.2Help
Entry
EC 4.1.99.2                 Enzyme                                 

Name
tyrosine phenol-lyase;
beta-tyrosinase;
L-tyrosine phenol-lyase (deaminating)
Class
Lyases;
Carbon-carbon lyases;
Other carbon-carbon lyases
BRITE hierarchy
Sysname
L-tyrosine phenol-lyase (deaminating; pyruvate-forming)
Reaction(IUBMB)
L-tyrosine + H2O = phenol + pyruvate + NH3 (overall reaction) [RN:R00728];
(1a) L-tyrosine = phenol + 2-aminoprop-2-enoate;
(1b) 2-aminoprop-2-enoate = 2-iminopropanoate (spontaneous);
(1c) 2-iminopropanoate + H2O = pyruvate + NH3 (spontaneous)
Reaction(KEGG)
Substrate
L-tyrosine [CPD:C00082];
H2O [CPD:C00001];
2-aminoprop-2-enoate [CPD:C02218];
2-iminopropanoate [CPD:C20904]
Product
phenol [CPD:C00146];
pyruvate [CPD:C00022];
NH3 [CPD:C00014];
2-aminoprop-2-enoate [CPD:C02218];
2-iminopropanoate [CPD:C20904]
Comment
A pyridoxal-phosphate protein. The enzyme cleaves a carbon-carbon bond, releasing phenol and an unstable enamine product that tautomerizes to an imine form, which undergoes a hydrolytic deamination to form pyruvate and ammonia. The latter reaction, which can occur spontaneously, can also be catalysed by EC 3.5.99.10, 2-iminobutanoate/2-iminopropanoate deaminase. The enzyme also slowly catalyses similar reactions with D-tyrosine, S-methyl-L-cysteine, L-cysteine, L-serine and D-serine.
History
EC 4.1.99.2 created 1972
Pathway
ec00350  Tyrosine metabolism
Orthology
K01668  tyrosine phenol-lyase
Genes
KQV: B8P98_13910
CKO: CKO_03467
CFD: CFNIH1_09255
CBRA: A6J81_13920
CWE: CO701_18800
CYO: CD187_21020
CIF: AL515_04345
CIE: AN232_00695
EBC: C2U52_25405
YMA: DA391_12585
PAM: PANA_3984(tpl)
PLF: PANA5342_p10091(tpl)
PAJ: PAJ_p0025(tpl)
PAQ: PAGR_p073
MMK: MU9_2951
LRI: NCTC12151_02412(tpl)
PMU: PM0811
PMV: PMCN06_0880(tpl)
PMP: Pmu_08910(tpl)
PMUL: DR93_1650
AAT: D11S_1940
AAN: D7S_01988
AACN: AANUM_2335
DNO: DNO_0442
PSF: PSE_0962(tnaA)
CTC: CTC_00818
STH: STH2842
DSY: DSY3653
DHD: Dhaf_1723
TAE: TepiRe1_2021(tpl)
PUF: UFO1_1418
PFT: JBW_03623
SALU: DC74_716
SALL: SAZ_04135(tnaA)
AOI: AORI_5366
AJA: AJAP_22805(tpl)
RCA: Rcas_0866
CAU: Caur_0573
CAG: Cagg_3438
TDE: TDE1118(tpl)
TPED: TPE_1499(tpl)
FNU: FN1988
PCRE: NCTC12858_00848(tpl_1)
PSAC: PSM36_2654(tpl)
MRO: MROS_1546
DTN: DTL3_0081(tpl)
BANA: BARAN1_0718(tpl)
 » show all
Taxonomy
Reference
1  [PMID:4908868]
  Authors
Kumagai H, Yamada H, Matsui H, Ohkishi H, Ogata K.
  Title
Tyrosine phenol lyase. I. Purification, crystallization, and properties.
  Journal
J Biol Chem 245:1767-72 (1970)
Reference
2  [PMID:4908869]
  Authors
Kumagai H, Yamada H, Matsui H, Ohkishi H, Ogata K.
  Title
Tyrosine phenol lyase. II. Cofactor requrements.
  Journal
J Biol Chem 245:1773-7 (1970)
Other DBs
ExplorEnz - The Enzyme Database: 4.1.99.2
IUBMB Enzyme Nomenclature: 4.1.99.2
ExPASy - ENZYME nomenclature database: 4.1.99.2
BRENDA, the Enzyme Database: 4.1.99.2
CAS: 9059-31-8

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