KEGG   ENZYME: 4.2.1.103Help
Entry
EC 4.2.1.103                Enzyme                                 

Name
cyclohexyl-isocyanide hydratase;
isonitrile hydratase;
N-cyclohexylformamide hydro-lyase
Class
Lyases;
Carbon-oxygen lyases;
Hydro-lyases
BRITE hierarchy
Sysname
N-cyclohexylformamide hydro-lyase (cyclohexyl-isocyanide-forming)
Reaction(IUBMB)
N-cyclohexylformamide = cyclohexyl isocyanide + H2O [RN:R05771]
Reaction(KEGG)
Substrate
N-cyclohexylformamide [CPD:C11519]
Product
cyclohexyl isocyanide [CPD:C11520];
H2O [CPD:C00001]
Comment
The enzyme from Pseudomonas putida strain N19-2 can also catalyse the hydration of other isonitriles to the corresponding N-substituted formamides. The enzyme has no metal requirements.
History
EC 4.2.1.103 created 2001
Pathway
ec00930  Caprolactam degradation
ec01120  Microbial metabolism in diverse environments
Orthology
K18199  cyclohexyl-isocyanide hydratase
Genes
LPV: HYN51_05745
SFO: Z042_02840
RAH: Rahaq_2239
RAQ: Rahaq2_2344
RAA: Q7S_11325
ROX: BV494_12435
DDA: Dd703_2109
LBQ: CKQ53_13760
PAM: PANA_1582
PLF: PANA5342_2643
PAJ: PAJ_0920
PFL: PFL_4109(inhA)
PPRO: PPC_4210
PFS: PFLU_2085
PFE: PSF113_1826(inhA)
PFC: PflA506_1987(inhA)
PFW: PF1751_v1c19050(inhA)
PFB: VO64_1327
PMAN: OU5_1432
PSEM: TO66_21510
PSOS: POS17_4209
PANR: A7J50_2095
PSIL: PMA3_20215
BMA: BMAA0232
BMAL: DM55_3487
BMAE: DM78_4670
BMAQ: DM76_4184
BMAI: DM57_14220
BMAF: DM51_3982
BMAZ: BM44_3449
BMAB: BM45_4285
BPS: BPSS1863
BPSE: BDL_5266
BPSM: BBQ_4269
BPSU: BBN_5326
BPSD: BBX_4431
BPK: BBK_4598
BPSH: DR55_5874
BPSA: BBU_4148
BPSO: X996_5671
BUT: X994_5235
BTQ: BTQ_3807
BTJ: BTJ_4838
BTZ: BTL_5633
BTD: BTI_5131
BTV: BTHA_4577
BTHE: BTN_5295
BTHM: BTRA_5566
BTHA: DR62_5433
BTHL: BG87_5640
BVE: AK36_1231
BCN: Bcen_2047
BCJ: BCAL0944
BCEN: DM39_2748
BCEW: DM40_285
BCEO: I35_2723
BAM: Bamb_2705
BMU: Bmul_0639
BMK: DM80_2260
BMUL: NP80_2794
BCT: GEM_0778
BCED: DM42_2413
BDL: AK34_430
BCON: NL30_26365
BUB: BW23_2234
BLAT: WK25_12910
BTEI: WS51_23710
BSEM: WJ12_13325
BPSL: WS57_32075
BMEC: WJ16_13640
BSTG: WT74_14115
BGU: KS03_2093
BGO: BM43_2691
BYI: BYI23_B003790(pfpI)
BUK: MYA_2423
BUE: BRPE67_BCDS06360(pfpI)
BUL: BW21_4667
ODI: ODI_R3652
LIH: L63ED372_02726(inhA)
CFU: CFU_1980
CGRA: CGRAC_1525
SMUL: SMUL_0876(inhA)
SULJ: SJPD1_0822
ARA: Arad_8694
AVI: Avi_1644
BJA: bll4280
BRA: BRADO3481
BBT: BBta_4230
AOL: S58_36400
BRAD: BF49_0765
RPA: RPA2422
RPB: RPB_3031
RPC: RPC_2887
RPT: Rpal_2700
VGO: GJW-30_1_00522(inhA_1)
AZC: AZC_2741
MEX: Mext_3318
MEA: Mex_1p3533(inh)
MDI: METDI4110(inh)
MCH: Mchl_3642
MPO: Mpop_3518
MET: M446_4875
MNO: Mnod_1849
MOR: MOC_2046
MAQU: Maq22A_c27365(thiJ)
FIL: BN1229_v1_1635(inhA)
FIY: BN1229_v1_1638(inhA)
DEQ: XM25_09205(ThiJ)
MSC: BN69_0478
MMED: Mame_03271(inhA)
MCG: GL4_0841
HDI: HDIA_2813(inhA_1)
PSF: PSE_1973
SPHP: LH20_15765
SMAZ: LH19_15905
SGI: SGRAN_1015(inhA)
SPHU: SPPYR_2980(inhA)
SPHI: TS85_18445
SFLA: SPHFLASMR4Y_02170(inhA)
BLAS: BSY18_1137
ADO: A6F68_00187(inhA)
AFR: AFE_2005
ACU: Atc_0156
PRI: PRIO_2794
PSWU: SY83_03965
ASOC: CB4_03049(inhA_2)
CPAT: CLPA_c08660(inhA2)
CPAE: CPAST_c08660(inhA2)
ACTI: UA75_06630
ACAD: UA74_06630
AHG: AHOG_06350(inhA1) AHOG_16250(inhA4)
GLJ: GKIL_2120(ftrA)
ANA: all7521
CTHE: Chro_3529
GES: VT84_24845(inhA)
LIE: LIF_A0444
LIS: LIL_13132
LST: LSS_00045
GBA: J421_1851
 » show all
Taxonomy
Reference
1  [PMID:11306561]
  Authors
Goda M, Hashimoto Y, Shimizu S, Kobayashi M.
  Title
Discovery of a novel enzyme, isonitrile hydratase, involved in nitrogen-carbon triple bond cleavage.
  Journal
J Biol Chem 276:23480-5 (2001)
DOI:10.1074/jbc.M007856200
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 4.2.1.103
IUBMB Enzyme Nomenclature: 4.2.1.103
ExPASy - ENZYME nomenclature database: 4.2.1.103
UM-BBD (Biocatalysis/Biodegradation Database): 4.2.1.103
BRENDA, the Enzyme Database: 4.2.1.103
CAS: 358974-06-8

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