KEGG   ENZYME: 4.2.1.111Help
Entry
EC 4.2.1.111                Enzyme                                 

Name
1,5-anhydro-D-fructose dehydratase;
1,5-anhydro-D-fructose 4-dehydratase;
1,5-anhydro-D-fructose hydrolyase;
1,5-anhydro-D-arabino-hex-2-ulose dehydratase;
AFDH;
AF dehydratase;
1,5-anhydro-D-fructose hydro-lyase
Class
Lyases;
Carbon-oxygen lyases;
Hydro-lyases
BRITE hierarchy
Sysname
1,5-anhydro-D-fructose hydro-lyase (ascopyrone-M-forming)
Reaction(IUBMB)
1,5-anhydro-D-fructose = 1,5-anhydro-4-deoxy-D-glycero-hex-3-en-2-ulose + H2O [RN:R09694]
Reaction(KEGG)
Substrate
1,5-anhydro-D-fructose [CPD:C06485]
Product
1,5-anhydro-4-deoxy-D-glycero-hex-3-en-2-ulose [CPD:C19822];
H2O [CPD:C00001]
Comment
This enzyme catalyses one of the steps in the anhydrofructose pathway, which leads to the degradation of glycogen and starch via 1,5-anhydro-D-fructose [1,2]. The other enzymes involved in this pathway are EC 4.2.1.110 (aldos-2-ulose dehydratase), EC 4.2.2.13 [exo-(1->4)-alpha-D-glucan lyase] and EC 5.3.2.7 (ascopyrone tautomerase). Requires divalent (Ca2+ or Mg2+) or monovalent cations (Na+) for optimal activity. Unlike EC 4.2.1.110, the enzyme is specific for 1,5-anhydro-D-fructose as substrate and shows no activity towards aldose-2-uloses such as 2-dehydroglucose [1,2,3]. In addition, it is inhibited by its end-product ascopyrone M [2] and it cannot convert ascopyrone M into microthecin, as can EC 4.2.1.110.
History
EC 4.2.1.111 created 2006
Reference
1  [PMID:15110094]
  Authors
Yu S, Refdahl C, Lundt I.
  Title
Enzymatic description of the anhydrofructose pathway of glycogen degradation; I. Identification and purification of anhydrofructose dehydratase, ascopyrone tautomerase and alpha-1,4-glucan lyase in the fungus Anthracobia melaloma.
  Journal
Biochim Biophys Acta 1672:120-9 (2004)
DOI:10.1016/j.bbagen.2004.03.004
Reference
2  [PMID:16822618]
  Authors
Yu S, Fiskesund R.
  Title
The anhydrofructose pathway and its possible role in stress response and signaling.
  Journal
Biochim Biophys Acta 1760:1314-22 (2006)
DOI:10.1016/j.bbagen.2006.05.007
Reference
3  [PMID:15716041]
  Authors
Yu S.
  Title
Enzymatic description of the anhydrofructose pathway of glycogen degradation II. Gene identification and characterization of the reactions catalyzed by aldos-2-ulose dehydratase that converts 1,5-anhydro-D-fructose to microthecin with ascopyrone M as the intermediate.
  Journal
Biochim Biophys Acta 1723:63-73 (2005)
DOI:10.1016/j.bbagen.2005.01.004
Other DBs
ExplorEnz - The Enzyme Database: 4.2.1.111
IUBMB Enzyme Nomenclature: 4.2.1.111
ExPASy - ENZYME nomenclature database: 4.2.1.111
UM-BBD (Biocatalysis/Biodegradation Database): 4.2.1.111
BRENDA, the Enzyme Database: 4.2.1.111

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