KEGG   ENZYME: 4.2.1.119Help
Entry
EC 4.2.1.119                Enzyme                                 

Name
enoyl-CoA hydratase 2;
2-enoyl-CoA hydratase 2;
AtECH2;
ECH2;
MaoC;
MFE-2;
PhaJAc;
D-3-hydroxyacyl-CoA hydro-lyase;
D-specific 2-trans-enoyl-CoA hydratase
Class
Lyases;
Carbon-oxygen lyases;
Hydro-lyases
BRITE hierarchy
Sysname
(3R)-3-hydroxyacyl-CoA hydro-lyase
Reaction(IUBMB)
(3R)-3-hydroxyacyl-CoA = (2E)-2-enoyl-CoA + H2O [RN:R09698]
Reaction(KEGG)
Substrate
(3R)-3-hydroxyacyl-CoA [CPD:C01086]
Product
(2E)-2-enoyl-CoA [CPD:C00658];
H2O [CPD:C00001]
Comment
This enzyme catalyses a hydration step in peroxisomal beta-oxidation. The human multifunctional enzyme type 2 (MFE-2) is a 79000 Da enzyme composed of three functional units: (3R)-hydroxyacyl-CoA dehydrogenase, 2-enoyl-CoA hydratase 2 and sterol carrier protein 2-like units [1]. The enzymes from Aeromonas caviae [4] and Arabidopsis thaliana [5] are monofunctional enzymes. 2-Enoyl-CoA hydratase 3 from Candida tropicalis is a part from multifunctional enzyme type 2 [3].
History
EC 4.2.1.119 created 2009
Orthology
K12405  3-hydroxyacyl-CoA dehydrogenase / 3a,7a,12a-trihydroxy-5b-cholest-24-enoyl-CoA hydratase / enoyl-CoA hydratase 2
K19658  peroxisomal enoyl-CoA hydratase 2
K19659  enoyl-CoA hydratase
Genes
HSA: 3295(HSD17B4)
PTR: 462019(HSD17B4)
PPS: 100981028(HSD17B4)
GGO: 101129538(HSD17B4)
PON: 100448539(HSD17B4)
NLE: 100590035(HSD17B4)
MCC: 698369(HSD17B4)
MCF: 102123675(HSD17B4)
CSAB: 103244395(HSD17B4)
RRO: 104654581(HSD17B4)
RBB: 108517761 108529825(HSD17B4)
CJC: 100390100(HSD17B4)
SBQ: 101036776(HSD17B4)
MMU: 15488(Hsd17b4)
RNO: 79244(Hsd17b4)
CGE: 103161104(Hsd17b4) 107980163
NGI: 103745113(Hsd17b4)
HGL: 101713520(Hsd17b4)
OCU: 100346781(HSD17B4)
TUP: 102468828(HSD17B4)
CFA: 474630(HSD17B4)
AML: 100471749(HSD17B4)
UMR: 103670554(HSD17B4)
ORO: 101385731(HSD17B4)
FCA: 101091840(HSD17B4)
PTG: 102968873(HSD17B4)
AJU: 106975089(HSD17B4)
BTA: 493643(HSD17B4)
BOM: 102268517(HSD17B4)
PHD: 102329357(HSD17B4)
CHX: 102172142(HSD17B4)
OAS: 101114519(HSD17B4)
SSC: 397574(HSD17B4)
CFR: 102508317(HSD17B4)
CDK: 105104293(HSD17B4)
LVE: 103084787(HSD17B4)
OOR: 101282963(HSD17B4)
ECB: 100034046(HSD17B4)
EPZ: 103548315(HSD17B4)
EAI: 106833039(HSD17B4)
MYB: 102240475(HSD17B4)
MYD: 102767159(HSD17B4)
HAI: 109374101(HSD17B4)
RSS: 109455167(HSD17B4)
PALE: 102887000(HSD17B4)
LAV: 100659299(HSD17B4)
TMU: 101356123
MDO: 100028098(HSD17B4)
SHR: 100932771(HSD17B4)
OAA: 100081754(HSD17B4)
GGA: 395785(HSD17B4)
MGP: 100545396(HSD17B4)
CJO: 107306670(HSD17B4)
APLA: 101791118(HSD17B4)
ACYG: 106048362(HSD17B4)
TGU: 100218226(HSD17B4)
GFR: 102036502(HSD17B4)
FAB: 101807233(HSD17B4)
PHI: 102109547(HSD17B4)
PMAJ: 107216347(HSD17B4)
CCW: 104690497(HSD17B4)
FPG: 101917400(HSD17B4)
FCH: 102058406(HSD17B4)
CLV: 102095771(HSD17B4)
EGZ: 104122491(HSD17B4)
ASN: 102371766(HSD17B4)
AMJ: 102571005(HSD17B4)
PSS: 102451304(HSD17B4)
CMY: 102933522(HSD17B4)
CPIC: 101945395(HSD17B4)
ACS: 100558484(hsd17b4)
PVT: 110077461(SCP2D1) 110084344(HSD17B4)
PBI: 103059748(SCP2D1) 103063870(HSD17B4)
GJA: 107106522(SCP2D1) 107116225(HSD17B4)
XLA: 444492(hsd17b4.L)
XTR: 613082(hsd17b4)
NPR: 108787696(HSD17B4)
DRE: 393105(hsd17b4)
SRX: 107713552(hsd17b4) 107713572
SANH: 107703629(hsd17b4)
SGH: 107571774(hsd17b4)
IPU: 108265438(hsd17b4)
AMEX: 103046836(hsd17b4)
TRU: 101062498(hsd17b4)
LCO: 104920820(hsd17b4)
NCC: 104966339(hsd17b4)
MZE: 101482787(hsd17b4)
OLA: 101159921(hsd17b4)
XMA: 102223774(hsd17b4)
PRET: 103470095(hsd17b4)
NFU: 107394149(hsd17b4)
CSEM: 103397642(hsd17b4)
LCF: 108882142(hsd17b4)
HCQ: 109526033(hsd17b4)
BPEC: 110167024(hsd17b4)
SASA: 106585849(hsd17b4)
ELS: 105014609(hsd17b4)
SFM: 108938133(hsd17b4)
LCM: 102356548(HSD17B4)
CMK: 103181119(hsd17b4)
CIN: 100187421(hsd17b4)
SPU: 581580(hsd17b4)
APLC: 110987677
DME: Dmel_CG3415(Mfe2)
DSI: Dsimw501_GD29376(Dsim_GD29376)
MDE: 101897498
AAG: 5568633
AME: 409986
BIM: 100745646
BTER: 100648641
SOC: 105192979
AEC: 105149946
ACEP: 105621202
HST: 105188161
CFO: 105248091
LHU: 105679879
PGC: 109854791
NVI: 100123018
TCA: 663655(17-beta-HD4)
DPA: 109536257
NVL: 108562871
BMOR: 101745499
PMAC: 106711197
PRAP: 110991438
PXY: 105391367
API: 100167575
DNX: 107163428
ZNE: 110835121
TUT: 107364381
CEL: CELE_E04F6.3(maoc-1) CELE_M03A8.1(dhs-28)
CBR: CBG10899(Cbr-dhs-28) CBG13089(Cbr-maoc-1)
CRG: 105326295
MYI: 110453744
OBI: 106871734
EPA: 110252238
ADF: 107337999
AQU: 100640903
ATH: AT1G76150(ECH2)
CRB: 17896162
BRP: 103832064
BOE: 106300265
THJ: 104813765
CPAP: 110818108
CIT: 102628415
TCC: 18591652
GRA: 105770003
GHI: 107904979
EGR: 104448895
VRA: 106762546
VAR: 108343556
CCAJ: 109802448
CAM: 101492992
LJA: Lj1g3v2268360.1(Lj1g3v2268360.1) Lj1g3v2268360.2(Lj1g3v2268360.2) Lj1g3v2953590.1(Lj1g3v2953590.1) Lj1g3v2953590.2(Lj1g3v2953590.2)
ADU: 107460260
AIP: 107609751
LANG: 109343989
FVE: 101293547
PPER: 18792035
PMUM: 103321173
PAVI: 110746650
MDM: 103440214
ZJU: 107422728
CSV: 101212798
CMO: 103488374
MCHA: 111014062
CMAX: 111492280
CMOS: 111462566
CPEP: 111806066
RCU: 8267862
JCU: 105635268
HBR: 110636119
POP: 7494669
JRE: 109018894
VVI: 100262653
SLY: 101253171
SPEN: 107005416
SOT: 102584477
CANN: 107850442
INI: 109184502
SIND: 105178076
OEU: 111379149
HAN: 110908076
LSV: 111889535
BVG: 104884406
SOE: 110790608
NNU: 104603702
OSA: 4347752
DOSA: Os09t0544900-01(Os09g0544900)
BDI: 100844320
ATS: 109758253(LOC109758253)
PDA: 103701068
EGU: 105055161
MUS: 103970614
AOF: 109848560
ATR: 18424521
PPP: 112281828
DDI: DDB_G0291247(mfeA)
DFA: DFA_02002(mfeA)
TGO: TGME49_234570(HAD2SCP2)
SMIN: v1.2.022875.t1(symbB.v1.2.022875.t1)
VTA: B0498(phaJ)
SPL: Spea_1753
SHL: Shal_1988
FBL: Fbal_0386
AHA: AHA_2422
ASA: ASA_2281(phaJ)
AMED: B224_2076
ACAV: VI35_11530
CARE: LT85_3600
 » show all
Taxonomy
Reference
1  [PMID:15644212]
  Authors
Koski KM, Haapalainen AM, Hiltunen JK, Glumoff T
  Title
Crystal structure of 2-enoyl-CoA hydratase 2 from human peroxisomal multifunctional enzyme type 2.
  Journal
J Mol Biol 345:1157-69 (2005)
DOI:10.1016/j.jmb.2004.11.009
  Sequence
[hsa:3295]
Reference
2  [PMID:9457873]
  Authors
Fukui T, Shiomi N, Doi Y
  Title
Expression and characterization of (R)-specific enoyl coenzyme A hydratase involved in polyhydroxyalkanoate biosynthesis by Aeromonas caviae.
  Journal
J Bacteriol 180:667-73 (1998)
  Sequence
Reference
3  [PMID:12832794]
  Authors
Koski MK, Haapalainen AM, Hiltunen JK, Glumoff T
  Title
Crystallization and preliminary crystallographic data of 2-enoyl-CoA hydratase 2  domain of Candida tropicalis peroxisomal multifunctional enzyme type 2.
  Journal
Acta Crystallogr D Biol Crystallogr 59:1302-5 (2003)
Reference
4  [PMID:11134939]
  Authors
Hisano T, Fukui T, Iwata T, Doi Y
  Title
Crystallization and preliminary X-ray analysis of (R)-specific enoyl-CoA hydratase from Aeromonas caviae involved in polyhydroxyalkanoate biosynthesis.
  Journal
Acta Crystallogr D Biol Crystallogr 57:145-7 (2001)
Reference
5  [PMID:16982622]
  Authors
Goepfert S, Hiltunen JK, Poirier Y
  Title
Identification and functional characterization of a monofunctional peroxisomal enoyl-CoA hydratase 2 that participates in the degradation of even cis-unsaturated fatty acids in Arabidopsis thaliana.
  Journal
J Biol Chem 281:35894-903 (2006)
DOI:10.1074/jbc.M606383200
  Sequence
[ath:AT1G76150]
Reference
6  [PMID:1879422]
  Authors
Engeland K, Kindl H
  Title
Evidence for a peroxisomal fatty acid beta-oxidation involving D-3-hydroxyacyl-CoAs. Characterization of two forms of hydro-lyase that convert D-(-)-3-hydroxyacyl-CoA into 2-trans-enoyl-CoA.
  Journal
Eur J Biochem 200:171-8 (1991)
DOI:10.1111/j.1432-1033.1991.tb21064.x
Other DBs
ExplorEnz - The Enzyme Database: 4.2.1.119
IUBMB Enzyme Nomenclature: 4.2.1.119
ExPASy - ENZYME nomenclature database: 4.2.1.119
BRENDA, the Enzyme Database: 4.2.1.119

DBGET integrated database retrieval system