KEGG   ENZYME: 4.2.1.120Help
Entry
EC 4.2.1.120                Enzyme                                 

Name
4-hydroxybutanoyl-CoA dehydratase
Class
Lyases;
Carbon-oxygen lyases;
Hydro-lyases
BRITE hierarchy
Sysname
4-hydroxybutanoyl-CoA hydro-lyase
Reaction(IUBMB)
4-hydroxybutanoyl-CoA = (E)-but-2-enoyl-CoA + H2O [RN:R10782]
Reaction(KEGG)
Substrate
4-hydroxybutanoyl-CoA [CPD:C11062]
Product
(E)-but-2-enoyl-CoA [CPD:C00877];
H2O [CPD:C00001]
Comment
Contains FAD and a [4Fe-4S] iron-sulfur cluster. The enzyme has been characterized from several microorganisms, including Clostridium kluyveri, where it participates in succinate fermentation [1,2], Clostridium aminobutyricum, where it participates in 4-aminobutyrate degradation [3,4], and Metallosphaera sedula, where it participates in the 3-hydroxypropionate/4-hydroxybutyrate cycle, an autotrophic CO2 fixation pathway found in some thermoacidophilic archaea [5].
History
EC 4.2.1.120 created 2009
Pathway
ec00650  Butanoate metabolism
ec00720  Carbon fixation pathways in prokaryotes
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K14534  4-hydroxybutyryl-CoA dehydratase / vinylacetyl-CoA-Delta-isomerase
Genes
BGJ: AWC36_04165
KAK: Kalk_18475
PNU: Pnuc_0370
ACHR: C2U31_22605
VPE: Varpa_3723
VPD: VAPA_1c23470
VAA: AX767_00840
VBO: CKY39_20160
VAM: C4F17_02945
RTA: Rta_18760
HYB: Q5W_11860
MPT: Mpe_A2516
LCH: Lcho_2993
METR: BSY238_78
GME: Gmet_2215
DEU: DBW_0157(abfD)
DOL: Dole_2592
DAL: Dalk_1215
DTO: TOL2_C28460(hpaB)
SAT: SYN_02445
SFU: Sfum_3141
DBR: Deba_0626
OCA: OCAR_6245
OCG: OCA5_c17860(hbaB)
OCO: OCA4_c17860(hbaB)
VGO: GJW-30_1_00655(abfD)
SMAZ: LH19_13280
BACO: OXB_3219
CBE: Cbei_2100
CBZ: Cbs_2100
CBEI: LF65_02412
CKL: CKL_3020(abfD)
CKR: CKR_2668
CPAS: Clopa_4009
CTYK: CTK_C07640(abfD)
CDF: CD630_23410(abfD)
PDC: CDIF630_02580(abfD)
CDC: CD196_2184(abfD)
CDL: CDR20291_2230(abfD)
PDF: CD630DERM_23410(abfD)
SLP: Slip_0662
PTH: PTH_1222
ELM: ELI_0281
OVA: OBV_01110(abfD) OBV_03760(abfD) OBV_28840(abfD) OBV_33370(abfD) OBV_35330(abfD) OBV_40730(abfD)
CHY: CHY_1314(abfD) CHY_1730
PED: ING2D1G_1210(abfD)
MED: MELS_0342
AIN: Acin_2034
PGI: PG_0692(abfD)
PGN: PGN_0727
PGT: PGTDC60_1817(abfD)
PCRE: NCTC12858_01801(abfD)
TFO: BFO_1484(abfD)
TTK: TST_0398(abfD)
AFU: AF_0333
GAC: GACE_1499
GAH: GAH_01688
IHO: Igni_0595
IIS: EYM_00450
STO: STK_16590
SSO: SSO2738(abfD-2)
SOL: Ssol_0550
SSOA: SULA_0541
SSOL: SULB_0543
SSOF: SULC_0541
SAI: Saci_2143(abfD)
SID: M164_2569
SII: LD85_2896
SIH: SiH_2522
SIR: SiRe_2362
SIC: SiL_2419
MSE: Msed_1321
MCN: Mcup_0888
AHO: Ahos_2036
PAI: PAE2693
PIS: Pisl_0248
PCL: Pcal_1396
PYR: P186_0718
POG: Pogu_1298
TNE: Tneu_0422
TUZ: TUZN_0949
TTN: TTX_1102
NMR: Nmar_0207
NID: NPIRD3C_0596(abfD)
NIN: NADRNF5_2050(abfD)
NCT: NMSP_1505
NEV: NTE_00645
TAA: NMY3_03315(abfD)
NCV: NCAV_0127
NBV: T478_1248(abfD)
NDV: NDEV_0181(hcd)
LOKI: Lokiarch_00630(abfD_1) Lokiarch_26350(abfD_2)
 » show all
Taxonomy
Reference
1  [PMID:13687513]
  Authors
BARTSCH RG, BARKER HA
  Title
A vinylacetyl isomerase from Clostridium kluyveri.
  Journal
Arch Biochem Biophys 92:122-32 (1961)
DOI:10.1016/0003-9861(61)90226-0
Reference
2  [PMID:8161284]
  Authors
Scherf U, Sohling B, Gottschalk G, Linder D, Buckel W
  Title
Succinate-ethanol fermentation in Clostridium kluyveri: purification and characterisation of 4-hydroxybutyryl-CoA dehydratase/vinylacetyl-CoA delta 3-delta 2-isomerase.
  Journal
Arch Microbiol 161:239-45 (1994)
  Sequence
[ckl:CKL_3020]
Reference
3  [PMID:8344309]
  Authors
Scherf U, Buckel W
  Title
Purification and properties of an iron-sulfur and FAD-containing 4-hydroxybutyryl-CoA dehydratase/vinylacetyl-CoA delta 3-delta 2-isomerase from Clostridium aminobutyricum.
  Journal
Eur J Biochem 215:421-9 (1993)
DOI:10.1111/j.1432-1033.1993.tb18049.x
  Sequence
Reference
4  [PMID:8794752]
  Authors
Muh U, Cinkaya I, Albracht SP, Buckel W
  Title
4-Hydroxybutyryl-CoA dehydratase from Clostridium aminobutyricum: characterization of FAD and iron-sulfur clusters involved in an overall non-redox reaction.
  Journal
Biochemistry 35:11710-8 (1996)
DOI:10.1021/bi9601363
Reference
5  [PMID:18079405]
  Authors
Berg IA, Kockelkorn D, Buckel W, Fuchs G
  Title
A 3-hydroxypropionate/4-hydroxybutyrate autotrophic carbon dioxide assimilation pathway in Archaea.
  Journal
Science 318:1782-6 (2007)
DOI:10.1126/science.1149976
Other DBs
ExplorEnz - The Enzyme Database: 4.2.1.120
IUBMB Enzyme Nomenclature: 4.2.1.120
ExPASy - ENZYME nomenclature database: 4.2.1.120
BRENDA, the Enzyme Database: 4.2.1.120

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