KEGG   ENZYME: 4.2.1.36Help
Entry
EC 4.2.1.36                 Enzyme                                 

Name
homoaconitate hydratase;
homoaconitase;
cis-homoaconitase;
HACN;
Lys4;
LysF;
2-hydroxybutane-1,2,4-tricarboxylate hydro-lyase (incorrect)
Class
Lyases;
Carbon-oxygen lyases;
Hydro-lyases
BRITE hierarchy
Sysname
(1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate hydro-lyase [(Z)-but-1-ene-1,2,4-tricarboxylate-forming]
Reaction(IUBMB)
(1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate = (Z)-but-1-ene-1,2,4-tricarboxylate + H2O [RN:R04371]
Reaction(KEGG)
Substrate
(1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate [CPD:C05662]
Product
(Z)-but-1-ene-1,2,4-tricarboxylate [CPD:C04002];
H2O [CPD:C00001]
Comment
Requires a [4Fe-4S] cluster for activity. The enzyme from the hyperthermophilic eubacterium Thermus thermophilus can catalyse the reaction shown above but cannot catalyse the previously described reaction, i.e. formation of (R)-homocitrate by hydration of cis-homoaconitate. The enzyme responsible for the conversion of cis-homoaconitate into (R)-homocitrate in T. thermophilus is unknown at present but the reaction can be catalysed in vitro using aconitate hydratase from pig (EC 4.2.1.3) [2].
History
EC 4.2.1.36 created 1972, modified 2007
Pathway
ec00300  Lysine biosynthesis
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
ec01130  Biosynthesis of antibiotics
Orthology
K01705  homoaconitate hydratase
Genes
SCE: YDR234W(LYS4)
AGO: AGOS_ABL106C
ERC: Ecym_5123
KLA: KLLA0_C15125g
KMX: KLMA_30527(LYS4)
LTH: KLTH0E10582g
VPO: Kpol_1031p57
ZRO: ZYRO0A10076g
CGR: CAGL0K10978g
NCS: NCAS_0B02660(NCAS0B02660)
NDI: NDAI_0C05030(NDAI0C05030)
TPF: TPHA_0L01150(TPHA0L01150)
TBL: TBLA_0A01460(TBLA0A01460)
TDL: TDEL_0B01800(TDEL0B01800)
KAF: KAFR_0E02730(KAFR0E02730)
PIC: PICST_88705(LYS4)
SPAA: SPAPADRAFT_58782(LYS4)
CAL: CAALFM_C404410CA(LYS4)
SLB: AWJ20_348(LYS4)
NCR: NCU08898(lys-6)
NTE: NEUTE1DRAFT58933(NEUTE1DRAFT_58933)
MGR: MGG_04842
SSCK: SPSK_03952
CMT: CCM_07793
BFU: BCIN_14g00430(Bclys4)
MBE: MBM_05638
ANI: AN6521.2
ANG: ANI_1_1106134(An15g00350)
ABE: ARB_04155
TVE: TRV_03936
PNO: SNOG_01131(SNOG_01129 SNOG_01130)
PTE: PTT_19520
SPO: SPAC343.16(lys2)
CNE: CNK00580
CNB: CNBK2910
ABP: AGABI1DRAFT59676(AGABI1DRAFT_59676)
ABV: AGABI2DRAFT184036(AGABI2DRAFT_184036)
MGL: MGL_3298
SPAR: SPRG_03993
IAL: IALB_1150
MRO: MROS_2220
CABY: Cabys_582
 » show all
Taxonomy
Reference
1  [PMID:5954805]
  Authors
Strassman M, Ceci LN.
  Title
Enzymatic formation of cis-homoaconitic acid, an intermediate in lysine biosynthesis in yeast.
  Journal
J Biol Chem 241:5401-7 (1966)
Reference
2  [PMID:16524361]
  Authors
Jia Y, Tomita T, Yamauchi K, Nishiyama M, Palmer DR.
  Title
Kinetics and product analysis of the reaction catalysed by recombinant homoaconitase from Thermus thermophilus.
  Journal
Biochem J 396:479-85 (2006)
DOI:10.1042/BJ20051711
Reference
3  [PMID:10714900]
  Authors
Zabriskie TM, Jackson MD.
  Title
Lysine biosynthesis and metabolism in fungi.
  Journal
Nat Prod Rep 17:85-97 (2000)
Other DBs
ExplorEnz - The Enzyme Database: 4.2.1.36
IUBMB Enzyme Nomenclature: 4.2.1.36
ExPASy - ENZYME nomenclature database: 4.2.1.36
BRENDA, the Enzyme Database: 4.2.1.36
CAS: 9030-68-6

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