KEGG   ENZYME: 4.2.1.80Help
Entry
EC 4.2.1.80                 Enzyme                                 

Name
2-oxopent-4-enoate hydratase;
2-keto-4-pentenoate hydratase;
OEH;
2-keto-4-pentenoate (vinylpyruvate)hydratase;
4-hydroxy-2-oxopentanoate hydro-lyase;
4-hydroxy-2-oxopentanoate hydro-lyase (2-oxopent-4-enoate-forming);
mhpD (gene name);
ahdF (gene name);
todG (gene name);
cmtF (gene name);
xylJ (gene name);
cnbE (gene name)
Class
Lyases;
Carbon-oxygen lyases;
Hydro-lyases
BRITE hierarchy
Sysname
(S)-4-hydroxy-2-oxopentanoate hydro-lyase ((2Z)-2-hydroxypenta-2,4-dienoate-forming)
Reaction(IUBMB)
(S)-4-hydroxy-2-oxopentanoate = (2Z)-2-hydroxypenta-2,4-dienoate + H2O [RN:R02601]
Reaction(KEGG)
R02601;
(other) R04781 R05297
Show
Substrate
(S)-4-hydroxy-2-oxopentanoate
Product
(2Z)-2-hydroxypenta-2,4-dienoate;
H2O [CPD:C00001]
Comment
The enzyme is involved in the catechol meta-cleavage pathway, a major mechanism for degradation of aromatic compounds. Also acts, more slowly, on cis-2-oxohex-4-enoate, but not on the trans-isomer. The enzyme was named when it was thought that the substrate is 2-oxopent-4-enoate. However, it was later found that the actual substrate is its tautomer (2Z)-2-hydroxypenta-2,4-dienoate. In some organisms the enzyme forms a complex with EC 4.1.1.77, 2-oxo-3-hexenedioate decarboxylase (previously named 4-oxalocrotonate decarboxylase).
History
EC 4.2.1.80 created 1984
Pathway
ec00360  Phenylalanine metabolism
ec00362  Benzoate degradation
ec00621  Dioxin degradation
ec00622  Xylene degradation
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K02554  2-keto-4-pentenoate hydratase
K18364  2-oxopent-4-enoate/cis-2-oxohex-4-enoate hydratase
Genes
ECO: b0350(mhpD)
ECJ: JW0341(mhpD)
EBW: BWG_0239(mhpD)
ECOK: ECMDS42_0272(mhpD)
ECE: Z0448(mhpD)
ECS: ECs0405(mhpD)
ECF: ECH74115_0425(mhpD)
ETW: ECSP_0414(mhpD)
ELX: CDCO157_0393(mhpD)
EOJ: ECO26_0386(mhpD)
EOI: ECO111_0386(mhpD)
EOH: ECO103_0332(mhpD)
ECOO: ECRM13514_0496(mhpD)
ECOH: ECRM13516_0330(mhpD)
EOK: G2583_0463(mhpD)
ELR: ECO55CA74_02225(mhpD)
ESO: O3O_05570(mhpD)
ESM: O3M_19710(mhpD)
ESL: O3K_19725(mhpD)
ECW: EcE24377A_0374(mhpD)
ELH: ETEC_0406
ECX: EcHS_A0414(mhpD)
ECM: EcSMS35_0381(mhpD)
ECY: ECSE_0375
ECR: ECIAI1_0351(mhpD)
ECQ: ECED1_0378(mhpD)
ECK: EC55989_0357(mhpD)
ECT: ECIAI39_0328(mhpD)
EOC: CE10_0318(mhpD)
EUM: ECUMN_0393(mhpD)
ELO: EC042_0387(mhpD)
ESE: ECSF_0321
EBR: ECB_00304(mhpD)
EBD: ECBD_3307
EKF: KO11_21830(mhpD)
EDJ: ECDH1ME8569_0337(mhpD)
ENA: ECNA114_0333(mhpD)
ELW: ECW_m0428(mhpD)
ELL: WFL_02105(mhpD)
ELP: P12B_c0310(mhpD) P12B_c0368(mhpD)
EBL: ECD_00304(mhpD)
EBE: B21_00308(mhpD)
ECOA: APECO78_05270(mhpD)
ECOJ: P423_01840
ECOS: EC958_0494
SSN: SSON_0329(mhpD)
KPN: KPN_02119(mhpD)
KPU: KP1_3214(mhpD)
KPP: A79E_2125
KPT: VK055_0342(mhpD)
KPE: KPK_2204(mhpD)
KPO: KPN2242_13550(mhpD)
KPR: KPR_2664(mhpD)
KPJ: N559_2171
KPX: PMK1_04483(mhpD)
KPNU: LI86_10785
KPNK: BN49_3224(mhpD)
KVA: Kvar_2156
KOX: KOX_22680(mhpD)
KOE: A225_3413
KOY: J415_14965(mhpD)
EBT: EBL_c20010(mhpD)
ROR: RORB6_04290(mhpD)
EBF: D782_1985
LRI: NCTC12151_03196(mhpD)
XCC: XCC0145(mhpD)
XCB: XC_0154
XCA: xcc-b100_0162(mhpD)
XCP: XCR_4394
XCV: XCV0146
XAX: XACM_0146
XAC: XAC0161(mhpD)
XCI: XCAW_00555(mhpD)
XFU: XFF4834R_chr00910(mhpD)
XPH: XppCFBP6546_11120(XppCFBP6546P_11120)
SACZ: AOT14_00290(mhpD)
PSUW: WQ53_07935
LAQ: GLA29479_2995(mhpD)
VEJ: VEJY3_06795(mhpD)
PPSE: BN5_3620(nahL)
PCQ: PcP3B5_23480(amnF) PcP3B5_25700(tesE_1)
PPI: ND086(nahL)
PPX: T1E_4256 T1E_4272(tobG)
PPUH: B479_25875(mhpD)
PPUT: L483_14065
PPUN: PP4_27260
PSYR: N018_13880
PPUU: PputUW4_01660(mhpD)
PSV: PVLB_26057(mhpD)
PKC: PKB_3259(mhpd1) PKB_3607(mhpd3) PKB_4239(xylJ)
PSOS: POS17_2263
AVN: Avin_08670(xylJ) Avin_15110(mhpD) Avin_30610(lapE)
AVL: AvCA_08670(xylJ) AvCA_15110(mhpD) AvCA_30610(lapE) AvCA_42150
ACX: Achr_27770(mhpD) Achr_32770(xylJ) Achr_32780
ASJ: AsACE_CH00350(mhpD)
SPL: Spea_2118
SHL: Shal_2090
SWD: Swoo_1392
PHA: PSHAa2141(xylJ)
PNG: PNIG_a2788(bphH)
AMAL: I607_15790
AMAE: I876_16090
AMAO: I634_16035
AMAD: I636_15935
AMAI: I635_16575
AMAG: I533_15630
SALH: HMF8227_02738(mhpD)
CYQ: Q91_0508
CYY: CPC19_10515(dmpE)
AEH: Mlg_2460
APAC: S7S_06990
AXE: P40_10440
KGE: TQ33_0085
TBN: TBH_C0928
GPB: HDN1F_27090(tesE)
RSO: RSp0891
RSM: CMR15_mp10875(mhpD)
RSE: F504_4326
RPI: Rpic_4620
RIN: ACS15_4354(dmpE) ACS15_4685(amnF)
REH: H16_B0548(mhpD1) H16_B0597(bphH) H16_B0884(mhpD3)
CNC: CNE_1c17860(mhpD2) CNE_2c04940(mhpD) CNE_2c05420(xylJ2) CNE_BB1p11990(xylJ1) CNE_BB1p13280(xylJ2)
RME: Rmet_1321(xylJ) Rmet_5207(mhpD)
CTI: RALTA_B0401(mhpD)
BVE: AK36_3500(mhpD)
BCJ: BCAM1613 BCAM2124(mhpD)
BCEN: DM39_1993(mhpD) DM39_3383(dmpE) DM39_4834(dmpE) DM39_4968(mhpD)
BCEW: DM40_5002(mhpD)
BCEO: I35_5473 I35_6006(mhpD)
BMUL: NP80_3432(mhpD) NP80_3870(amnF)
BCED: DM42_4993(dmpE) DM42_6345(dmpE)
BLAT: WK25_27490
BSEM: WJ12_26050
BUK: MYA_5004
BUL: BW21_2673(mhpD)
BXB: DR64_1247(amnF) DR64_3308(mhpD) DR64_4659(mhpD) DR64_8616(dmpE)
BPH: Bphy_4537
BFN: OI25_257(amnF) OI25_4589(dmpE)
BAV: BAV0598(bphE)
BHZ: ACR54_00826(mhpD)
TAS: TASI_0989
TAT: KUM_0455
AFQ: AFA_13950
ODI: ODI_R1483
POL: Bpro_5134
PNA: Pnap_4144
AJS: Ajs_0222
ACK: C380_15905(bphE)
VEI: Veis_2785
MELA: C6568_07860(dmpE)
JAG: GJA_236(xylJ)
LCH: Lcho_3343
DEY: HYN24_14130(dmpE) HYN24_14215(dmpE)
AZO: azo1853(lapE) azo1974(mhpD) azo2434(nahL)
THK: CCZ27_10770(dmpE) CCZ27_16275(dmpE)
ARC: ABLL_2444
ARA: Arad_7264
BRAD: BF49_2647
VGO: GJW-30_1_02000(mhpD_1)
XAU: Xaut_0936
MSL: Msil_1477
BVR: BVIR_1068
MMED: Mame_00144(mhpD)
RBM: TEF_21410
CCR: CC_1509
CAK: Caul_1767
CSE: Cseg_1571
PZU: PHZ_c2453(mhpD)
HBA: Hbal_2819
SAL: Sala_3044
SGI: SGRAN_0156 SGRAN_1420(mhpD2) SGRAN_1581(mhpD)
SPHM: G432_01985
SPHI: TS85_17615
SPKC: KC8_03560
SJP: SJA_C1-11870(mhpD)
SPHB: EP837_03394(bphH)
SPHR: BSY17_3779
SINB: SIDU_08330
SFLA: SPHFLASMR4Y_02368(tesE)
BLAS: BSY18_3297
GDI: GDI2732(mhpD)
TMO: TMO_b0121(todJ)
TXI: TH3_13610
MAGQ: MGMAQ_0649
BCA: BCE_2155
BCX: BCA_2153
BCF: bcf_10205
BCER: BCK_24230
BTL: BALH_1842(pheD)
BMYC: DJ92_4636
BMQ: BMQ_3589
BMD: BMD_3574
BMEG: BG04_463
BACO: OXB_2504
BKW: BkAM31D_08365(tesE)
GTN: GTNG_3153(nbaH)
ASOC: CB4_02450(tesE)
AAC: Aaci_1621
AAD: TC41_1517(mhpD)
BTS: Btus_0967
PTH: PTH_0481
TMR: Tmar_1784
MTA: Moth_1777
TOC: Toce_0376
MTU: Rv3536c(hsaE)
MTC: MT3640(mhpD)
MRA: MRA_3575(mhpD)
MTUR: CFBS_3752(mhpD)
MTD: UDA_3536c
MTUB: MT7199_3598(hasE)
MTUE: J114_18910
MTUH: I917_24815
MTUL: TBHG_03476
MTUT: HKBT1_3737(mhpD)
MTUU: HKBT2_3746(mhpD)
MTQ: HKBS1_3749(mhpD)
MBO: BQ2027_MB3566C(hsae)
MBB: BCG_3600c
MBT: JTY_3601
MBX: BCGT_3402
MAF: MAF_35480
MMIC: RN08_3912
MCQ: BN44_110028(mhpD)
MCV: BN43_90034(mhpD)
MCX: BN42_90031(mhpD)
MCZ: BN45_100031(mhpD)
MPA: MAP_0531
MAO: MAP4_3336
MAVI: RC58_16545
MAVU: RE97_16580
MAV: MAV_0625
MID: MIP_00989
MYO: OEM_05420
MIR: OCQ_05500
MLP: MLM_0770
MUL: MUL_4097
MMI: MMAR_5023
MMM: W7S_02620
MHAD: B586_04630
MSG: MSMEI_5780(mhpD)
MGI: Mflv_1520
MPHL: MPHLCCUG_00565(tesE)
MVQ: MYVA_5131
MTHN: 4412656_04052(mhpD)
MAB: MAB_0624
MABB: MASS_0594
MCHE: BB28_02990
MSTE: MSTE_00583
MTER: 4434518_03653(mhpD)
ASD: AS9A_1022
CDR: CDHC03_1947(mhpD)
CMV: CMUST_15060(mhpD)
CAMG: CAMM_02605
NFR: ERS450000_00751(mhpD_1) ERS450000_01022(mhpD_2) ERS450000_03772(mhpD_4)
NCY: NOCYR_0493(mhpD)
NSR: NS506_01254(bphH)
REQ: REQ_42080
RRT: 4535765_04743(cmtF)
GBR: Gbro_0224
GOR: KTR9_0810(hsaE)
TPR: Tpau_3798
SCB: SCAB_15211(bphE) SCAB_2091(mhpD)
SDV: BN159_1390(mhpD)
SALS: SLNWT_6913
SALU: DC74_7438
SALL: SAZ_38395
SLAU: SLA_6901
SLX: SLAV_27395(mhpD)
KSK: KSE_23730
ARX: ARZXY2_4005(mhpD)
NDK: I601_0559(mhpD)
TCU: Tcur_0534
SRO: Sros_9115
NML: Namu_4085
AMD: AMED_8623
AMN: RAM_44255
AMM: AMES_8492
AMZ: B737_8493
AOI: AORI_7402(mhpD)
KAL: KALB_5138
SAQ: Sare_3902
MIL: ML5_3355
AFS: AFR_23755
CWO: Cwoe_0439
RCA: Rcas_2405
CAU: Caur_1357
CAG: Cagg_2039
TRO: trd_1387
TTJ: TTHB239
GES: VT84_10640(tesE)
PBOR: BSF38_00915(mhpD)
AALG: AREALGSMS7_03659(amnF)
 » show all
Taxonomy
Reference
1  [PMID:7287632]
  Authors
Kunz DA, Ribbons DW, Chapman PJ.
  Title
Metabolism of allylglycine and cis-crotylglycine by Pseudomonas putida (arvilla) mt-2 harboring a TOL plasmid.
  Journal
J Bacteriol 148:72-82 (1981)
Reference
2  [PMID:2681159]
  Authors
Harayama S, Rekik M, Ngai KL, Ornston LN
  Title
Physically associated enzymes produce and metabolize 2-hydroxy-2,4-dienoate, a chemically unstable intermediate formed in catechol metabolism via meta cleavage  in Pseudomonas putida.
  Journal
J Bacteriol 171:6251-8 (1989)
DOI:10.1128/JB.171.11.6251-6258.1989
Reference
3  [PMID:9492273]
  Authors
Pollard JR, Bugg TD
  Title
Purification, characterisation and reaction mechanism of monofunctional 2-hydroxypentadienoic acid hydratase from Escherichia coli.
  Journal
Eur J Biochem 251:98-106 (1998)
  Sequence
[eco:b0350]
Other DBs
ExplorEnz - The Enzyme Database: 4.2.1.80
IUBMB Enzyme Nomenclature: 4.2.1.80
ExPASy - ENZYME nomenclature database: 4.2.1.80
UM-BBD (Biocatalysis/Biodegradation Database): 4.2.1.80
BRENDA, the Enzyme Database: 4.2.1.80
CAS: 64427-80-1

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