KEGG   ENZYME: 4.2.1.83Help
Entry
EC 4.2.1.83                 Enzyme                                 

Name
4-oxalomesaconate hydratase;
4-oxalmesaconate hydratase;
4-carboxy-2-oxohexenedioate hydratase;
4-carboxy-2-oxobutane-1,2,4-tricarboxylate 2,3-hydro-lyase;
oxalmesaconate hydratase;
gamma-oxalmesaconate hydratase;
2-hydroxy-4-oxobutane-1,2,4-tricarboxylate 2,3-hydro-lyase;
LigJ;
GalB
Class
Lyases;
Carbon-oxygen lyases;
Hydro-lyases
BRITE hierarchy
Sysname
(1E,3E)-4-hydroxybuta-1,3-diene-1,2,4-tricarboxylate 1,2-hydro-lyase (2-hydroxy-4-oxobutane-1,2,4-tricarboxylate-forming)
Reaction(IUBMB)
2-hydroxy-4-oxobutane-1,2,4-tricarboxylate = (1E,3E)-4-hydroxybuta-1,3-diene-1,2,4-tricarboxylate + H2O [RN:R04478]
Reaction(KEGG)
Substrate
2-hydroxy-4-oxobutane-1,2,4-tricarboxylate [CPD:C04115]
Product
(1E,3E)-4-hydroxybuta-1,3-diene-1,2,4-tricarboxylate [CPD:C04324];
H2O [CPD:C00001]
Comment
This enzyme participates in the degradation of 3,4-dihydroxybenzoate (via the meta-cleavage pathway), syringate and 3,4,5-trihydroxybenzoate, catalysing the reaction in the opposite direction [1-3]. It accepts the enol-form of 4-oxalomesaconate, 2-hydroxy-4-carboxy-hexa-2,4-dienedioate [4].
History
EC 4.2.1.83 created 1986, modified 2011
Pathway
ec00362  Benzoate degradation
ec01120  Microbial metabolism in diverse environments
Orthology
K10220  4-oxalmesaconate hydratase
K16515  4-oxalomesaconate hydratase
Genes
KPN: KPN_04049
KPU: KP1_5418
KPM: KPHS_52190
KPP: A79E_0058
KPK: A593_10480
KPH: KPNIH24_25965
KPZ: KPNIH27_24950
KPV: KPNIH29_25910
KPW: KPNIH30_26305
KPY: KPNIH31_25380
KPE: KPK_0044
KPR: KPR_0058
KPJ: N559_0094
KPX: PMK1_01555(galB)
KPNU: LI86_00290
KPNK: BN49_0058
KVA: Kvar_0050
CRO: ROD_31641
CAMA: F384_17450
YPE: YPO0083
YPK: y0054
YPH: YPC_0245
YPA: YPA_3458
YPN: YPN_3765
YPM: YP_0087
YPZ: YPZ3_0076
YPD: YPD4_0077
YPX: YPD8_0079
YPW: CH59_2177
YPJ: CH55_3044
YPV: BZ15_3485
YPL: CH46_831
YPS: YPTB0079
YPO: BZ17_2516
YPY: YPK_4120
YPB: YPTS_0083
YPQ: DJ40_2344
YPU: BZ21_3422
YPR: BZ20_2042
YPC: BZ23_3695
YPF: BZ19_3541
YMA: DA391_08515(galB)
SPE: Spro_2092
SMAF: D781_1919
RAA: Q7S_13285
EAM: EAMY_1949
EAY: EAM_1906
EBI: EbC_05360
XCC: XCC4032(fldW)
XCB: XC_4121
XCP: XCR_0228
XCV: XCV4255
XAX: XACM_4027
XAC: XAC4157(fldW)
XCI: XCAW_00141(fldW)
XOO: XOO4559(fldW)
XOP: PXO_03238
XOR: XOC_0383
XPH: XppCFBP6546_13615(XppCFBP6546P_13615)
PCQ: PcP3B5_13900(galB)
PPU: PP_2515(gllB)
PPI: YSA_00538
PPX: T1E_3399
PPUT: L483_20170
PPUN: PP4_26430
PSTT: CH92_18145
PSIL: PMA3_18980
PAT: Patl_3888
MBS: MRBBS_1268(acmsd)
CSA: Csal_0330
SALN: SALB1_0064
PSE: NH8B_2724
RSL: RPSI07_mp1416(ligJ)
RME: Rmet_3705(ligJ) Rmet_4204
BPSE: BDL_4882
BPSD: BBX_4044
BPK: BBK_4185
BCEN: DM39_6317(ligJ) DM39_6516(ligJ) DM39_6583(ligJ)
BCEO: I35_7833
BMU: Bmul_6039
BMK: DM80_3763(ligJ) DM80_6490(ligJ)
BMUL: NP80_4019(ligJ) NP80_5251(ligJ)
BCED: DM42_7334(ligJ)
BUB: BW23_4781(ligJ)
BTEI: WS51_29480
BPSL: WS57_17840
BGU: KS03_5339
BGO: BM43_447(ligJ) BM43_6954(ligJ)
BXE: Bxe_B2314
BXB: DR64_4646(ligJ)
BFN: OI25_4783
PPNO: DA70_15100
PPNM: LV28_15580
PAPI: SG18_10125
BPA: BPP1041
BPAR: BN117_3611
BBR: BB1257
BPT: Bpet3606
BHO: D560_0870(ligJ)
BHM: D558_0856(ligJ)
AMIM: MIM_c36760
AFQ: AFA_05535
ODI: ODI_R2305
RFR: Rfer_0338
POL: Bpro_4320
PNA: Pnap_2032
ACRA: BSY15_1208(ligJ)
VEI: Veis_0168
DAC: Daci_4449
CTES: O987_16285
RTA: Rta_24800
HYR: BSY239_2226(ligJ)
HYB: Q5W_10760
CBAA: SRAA_1354
CBAB: SMCB_0791
LCH: Lcho_3632
AZO: azo2542(fldW)
AOA: dqs_2699
TCL: Tchl_1058
SME: SMc00503
SMER: DU99_09650
ARA: Arad_2918
RLE: RL2853 pRL120276(ligJ)
RHT: NT26_3184
BJA: blr3841
BRA: BRADO2337(ligJ)
BBT: BBta_2697(ligJ)
BRS: S23_44020
AOL: S58_52620
BRAD: BF49_1200
BRO: BRAD285_5000(ligJ)
RPA: RPA4697(ligJ)
RPB: RPB_0877
RPD: RPD_0987
RPE: RPE_0746
RPT: Rpal_5178
AZC: AZC_3352
MOR: MOC_5210
PHL: KKY_3763
HDI: HDIA_4232
PDE: Pden_3274
MALG: MALG_02284
AHT: ANTHELSMS3_03532(galB)
HBA: Hbal_2605
SMAZ: LH19_17700
SPHD: HY78_10260
SSAN: NX02_17510
SSY: SLG_12520(ligJ)
SPHB: EP837_03491(ligJ)
SPHR: BSY17_3448
SINB: SIDU_17295
BLAS: BSY18_3522
AAY: WYH_01906
AMV: ACMV_19660(ligJ)
MAG: amb0251
MAGX: XM1_1087(ligJ)
MAGN: WV31_13595
MAGQ: MGMAQ_2666
BEO: BEH_09085
AAC: Aaci_1612
AAD: TC41_1507
ECAS: ECBG_00910
LPIL: LIP_2359
ROP: ROP_37240
GOR: KTR9_0702
SFA: Sfla_0531
SALB: XNR_3395
STRP: F750_6351
SLE: sle_08640(sle_08640)
STRD: NI25_00240
SLAU: SLA_7095
KSK: KSE_75170(pcmD)
AMIN: AUMI_19410
ART: Arth_4376
ACH: Achl_3361
BSD: BLASA_2608(ligJ)
MMAR: MODMU_2825
PDX: Psed_3700
PSEA: WY02_25110
PSEE: FRP1_23155
ACTN: L083_3808
CAI: Caci_3915
RXY: Rxyl_0201
TRO: trd_0904
STI: Sthe_2849
MRB: Mrub_2678
SUS: Acid_6637
SLI: Slin_4022
 » show all
Taxonomy
Reference
1  [PMID:6841354]
  Authors
Maruyama K
  Title
Enzymes responsible for degradation of 4-oxalmesaconic acid in Pseudomonas ochraceae.
  Journal
J Biochem 93:567-74 (1983)
Reference
2  [PMID:3985968]
  Authors
Maruyama K
  Title
Purification and properties of gamma-oxalomesaconate hydratase from Pseudomonas ochraceae grown with phthalate.
  Journal
Biochem Biophys Res Commun 128:271-7 (1985)
DOI:10.1016/0006-291X(85)91674-2
Reference
3  [PMID:11092855]
  Authors
Hara H, Masai E, Katayama Y, Fukuda M
  Title
The 4-oxalomesaconate hydratase gene, involved in the protocatechuate 4,5-cleavage pathway, is essential to vanillate and syringate degradation in Sphingomonas paucimobilis SYK-6.
  Journal
J Bacteriol 182:6950-7 (2000)
DOI:10.1128/JB.182.24.6950-6957.2000
Reference
4  [PMID:21219457]
  Authors
Nogales J, Canales A, Jimenez-Barbero J, Serra B, Pingarron JM, Garcia JL, Diaz E
  Title
Unravelling the gallic acid degradation pathway in bacteria: the gal cluster from Pseudomonas putida.
  Journal
Mol Microbiol 79:359-74 (2011)
DOI:10.1111/j.1365-2958.2010.07448.x
  Sequence
[ppu:PP_2515]
Other DBs
ExplorEnz - The Enzyme Database: 4.2.1.83
IUBMB Enzyme Nomenclature: 4.2.1.83
ExPASy - ENZYME nomenclature database: 4.2.1.83
BRENDA, the Enzyme Database: 4.2.1.83
CAS: 85204-95-1

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